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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||2 Antibodies||20 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies|
|Western Blotting (WB)||10 Antibodies|
|Blocking Antibody (Inhibition)||1 Antibodies|
|Blocking Peptide (BP)||1 Antibodies|
|Cell Culture (CC)||1 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Functional Studies (Func)||2 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Antigen||Colony Stimulating Factor 1 (Macrophage) (CSF1) Antibodies|
|Epitope||AA 33-262 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CSF1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
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Product Details anti-CSF1 AntibodyTarget Details CSF1 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Macrophage colony-stimulating factor 1(CSF1) detection. Tested with IHC-P, ELISA in Mouse,Rat.
Gene Name: colony stimulating factor 1 (macrophage)
Protein Name: Macrophage colony-stimulating factor 1
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived mouse MCSF recombinant protein (Position: K33-E262). Mouse MCSF shares 80.9 % and 88.6% amino acid (aa) sequence identity with human and rat MCSF, respectively.|
Target Details CSF1Product Details anti-CSF1 Antibody Application Details Handling Images back to top
|Alternative Name||CSF1 (CSF1 Antibody Abstract)|
|Background||M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival ofmonocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor (CSF1R or M-CSF-R encoded by the c-fms proto-oncogene), M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.|
Application DetailsProduct Details anti-CSF1 Antibody Target Details CSF1 Handling Images back to top
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
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|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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