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Human CSF1 ELISA Kit

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Antigen
Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kits
  • C87615
  • CSF-1
  • CSF1
  • csf1-1
  • Csfm
  • MCSF
  • op
  • zgc:172186
Epitope
AA 33-190
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
25
20
6
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN411321
$ 320.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32748017 ABIN414905 Sandwich ELISA Serum, Plasma, Urine, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32748017 ABIN1115841 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32748017 ABIN1379980 Sandwich ELISA Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.32748017 ABIN2708315 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate, Urine 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.32748017 ABIN2113595 Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (EDTA - heparin), Saliva, Urine Log in to see
0.0012092679 ABIN3032957 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 2.7-2000 pg/mL 2.7 pg/mL Log in to see
0.0012092679 ABIN625043 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 6
0.0012092679 ABIN511001 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see
0.0012092679 ABIN3198595 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012092679 ABIN1029180 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012092679 ABIN2683918 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Saliva, Serum, Tissue Homogenate, Urine 62.5-4000 pg/mL 62.5 pg/mL Log in to see 3
0.0012092679 ABIN1112744 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2042585 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2114494 Cell Lysate, Serum, Plasma 16-1000 pg/mL 16 pg/mL Log in to see
0.0012092679 ABIN2683919 Sandwich ELISA Cell Lysate, Tissue Lysate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2112004 Sandwich ELISA 120-4000 pg/mL 120 pg/mL Log in to see
0.0012092679 ABIN2683920 Sandwich ELISA Cell Samples Log in to see
0.0012092679 ABIN1846992 Serum, Plasma, Biological Fluids 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012092679 ABIN2543214 Sandwich ELISA Serum, Plasma, Urine, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.0012092679 ABIN2536286 Serum, Plasma, Biological Fluids 1-80 pg/mL 1 pg/mL Log in to see

Top referenced CSF1 ELISA Kit for ELISA

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Application / Reactivity Human
ELISA 25 ELISA Kits

General

Antigen Colony Stimulating Factor 1 (Macrophage) (CSF1) ELISA Kits
Epitope AA 33-190
Reactivity Human
Kits with alternative reactivity to:
(25), (20), (6), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details CSF1 ELISA Kit

Target details Application Details Handling References for CSF1 Kit (ABIN411321) Images
Purpose For quantitative detection of human M-CSF in cell culture supernates, tissue homogenates, serum, plasma (heparin, EDTA), saliva and urine.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Tissue Homogenate, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
Detection Method Colorimetric
Specificity Natural and recombinant human M-CSF
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human M-CSF antibod - 1
  • Lyophilized recombinant human M-CSF standar - 10ng/tubex2
  • Biotinylated anti- human M-CSF antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: E33-S190

Target details

Product Details CSF1 ELISA Kit Application Details Handling References for CSF1 Kit (ABIN411321) Images back to top
Antigen
Alternative Name CSF1 (CSF1 ELISA Kit Abstract)
Background M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
Gene ID 1435
UniProt P09603
Pathways RTK Signaling

Application Details

Product Details CSF1 ELISA Kit Target details Handling References for CSF1 Kit (ABIN411321) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human M-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for M-CSF has been precoated onto 96-well plates. Standards(E.coli, E33-S190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for M-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human M-CSF amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human M-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue lysates, serum, plasma (heparin or EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human M-CSF standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 546, Standard deviation: 36.6, CV(%): 6.7
  • Sample 2: n=16, Mean(pg/ml): 1011, Standard deviation: 44.5, CV(%): 4.4
  • Sample 3: n=16, Mean(pg/ml): 2456, Standard deviation: 125.3, CV(%): 5.1,
  • Sample 1: n=24, Mean(pg/ml): 623, Standard deviation: 43.61, CV(%): 7
  • Sample 2: n=24, Mean(pg/ml): 1528, Standard deviation: 85.57, CV(%): 5.6
  • Sample 3: n=24, Mean(pg/ml): 2629, Standard deviation: 152.5, CV(%): 5.8
Restrictions For Research Use only

Handling

Product Details CSF1 ELISA Kit Target details Application Details References for CSF1 Kit (ABIN411321) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CSF1 Kit (ABIN411321)

Product Details CSF1 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Ladner, Martin, Noble, Nikoloff, Tal, Kawasaki, White: "Human CSF-1: gene structure and alternative splicing of mRNA precursors." in: The EMBO journal, Vol. 6, Issue 9, pp. 2693-8, 1988

Images

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Supplier Images
ELISA image for CSF1 ELISA Kit (Colony Stimulating Factor 1 (Macrophage)) (ABIN411321) Human M-CSF PicoKine ELISA Kit standard curve