anti-Human CSF1R antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CSF1R Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
  • MGC89146
  • C-FMS
  • CD115
  • CSF-1R
  • CSFR
  • FIM2
  • FMS
  • HDLS
  • M-CSF-R
  • AI323359
  • Csfmr
  • Fim-2
  • Fms
  • M-CSFR
  • CSF-1-R
  • c-fms
  • mrfms
  • CSF1
  • M-CSF
  • csf1r
  • fms
  • panther
  • colony stimulating factor 1 receptor
  • csf1r protein
  • macrophage colony-stimulating factor receptor
  • colony stimulating factor 1 receptor, a
  • CSF1R
  • csf1r
  • m-csfr
  • Csf1r
  • csf1ra
Epitope
AA 694-744, pTyr723
40
38
36
26
24
21
20
19
14
12
11
10
9
8
7
6
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
359
219
85
22
12
9
8
6
4
3
3
3
2
2
2
1
Host
Rabbit
230
130
122
34
5
Clonality
Polyclonal
Conjugate
This CSF1R antibody is un-conjugated
37
32
26
22
18
15
13
12
11
9
8
8
8
7
7
5
5
5
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
309
233
227
155
82
57
28
16
15
12
10
8
6
5
4
3
3
3
3
2
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN683788
$ 317.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.676567 ABIN213686 IHC (p) Rabbit pTyr723 Log in to see Polyclonal
13.676567 ABIN498117 IF IHC (p) Rabbit Log in to see Polyclonal
13.676567 ABIN4332254 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
13.676567 ABIN4908358 IHC (p) WB Mouse IgG1 kappa Log in to see 1486CT328-53-37
1 ABIN1687668 IHC (p) Rabbit IgG Log in to see SP211 1
1 ABIN5610878 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN1687748 IHC (p) Rabbit IgG Log in to see SP211
1 ABIN2888738 IHC (p) ELISA WB Rabbit IgG AA 691-740 Log in to see Polyclonal
1 ABIN317788 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN317880 IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2888812 IF IHC (p) ELISA Rabbit IgG AA 531-580 Log in to see Polyclonal
1 ABIN2888811 IF IHC (p) ELISA WB Rabbit IgG AA 781-830 Log in to see Polyclonal
1 ABIN683797 IHC (p) WB HRP Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN1995873 ELISA IHC (p) Rabbit IgG AA 543-922 Log in to see Polyclonal
1 ABIN4332251 CyTOF ELISA FACS IHC IHC (p) WB Mouse IgG2b Log in to see 6B9H2
1 ABIN4332252 ELISA ICC IF IHC IHC (p) WB Mouse IgG2b Log in to see 6B9B9
1 ABIN683790 IHC (p) WB Biotin Rabbit IgG AA 694-744, pTyr723 Log in to see Polyclonal
1 ABIN2883677 ELISA IHC (p) WB Rabbit IgG pTyr561 Log in to see Polyclonal
1 ABIN2892734 IHC (p) WB Rabbit Gln718 Log in to see Polyclonal
1 ABIN2884033 ELISA IHC (p) WB Rabbit IgG pTyr723 Log in to see Polyclonal

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
Epitope AA 694-744, pTyr723
(40), (38), (36), (26), (24), (21), (20), (19), (14), (12), (11), (10), (9), (8), (7), (6), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(359), (219), (85), (22), (12), (9), (8), (6), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(230), (130), (122), (34), (5)
Clonality
Polyclonal
Conjugate This CSF1R antibody is un-conjugated
(37), (32), (26), (22), (18), (15), (13), (12), (11), (9), (8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(309), (233), (227), (155), (82), (57), (28), (16), (15), (12), (10), (8), (6), (5), (4), (3), (3), (3), (3), (2), (2)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CSF1R Antibody

Target Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN683788) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 723
Isotype IgG

Target Details CSF1R

Product Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R antibody (ABIN683788) Images back to top
Antigen
Alternative Name Csf1r (CSF1R Antibody Abstract)
Background

This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Subcellular location: Cytoplasm

Synonyms: FMS, CSFR, FIM2, HDLS, C-FMS, CD115, CSF-1R, M-CSF-R, Macrophage colony-stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, Proto-oncogene c-Fms, CSF1R

Gene ID 1436
UniProt P07333
Pathways RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization

Application Details

Product Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R antibody (ABIN683788) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R antibody (ABIN683788) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CSF1R antibody (ABIN683788)

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
Product cited in:

Król, Majchrzak, Mucha, Homa, Bulkowska, Jakubowska, Karwicka, Paw?owski, Motyl: "CSF-1R as an inhibitor of apoptosis and promoter of proliferation, migration and invasion of canine mammary cancer cells." in: BMC veterinary research, Vol. 9, pp. 65, 2013 Method employed by authors: Immunohistochemistry (IHC) Further details: After 30 min incubation in 5% bovine serum albumin (Sigma Aldrich) the rabbit polyclonal anti- p-CSF-1R (Tyr 723) antibodies diluted in 1% bovine serum were used, and then the slides were incubated at +4�C overnight.

Images

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN683788) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 694-744), (pTyr723) antibody (ABIN683788) Staining of canine lymph node tissue with bs-3077r. The primary antibody bs-3077r was...
'Independent Validation' Badge
Antigen CSF1R (Colony Stimulating Factor 1 Receptor)
Lot Number 9I02V3
Method validated Immunohistochemistry
Positive Control canine lymph node tissue
Negative Control secondary antibody only
Notes The CSF1R antibody ABIN683788 specifically labels the targeted antigen in canine lymph node samples in IHC.
Primary Antibody ABIN683788
Secondary Antibody anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712)
Protocol
  • Canine lymph node tissue is dissected and fixed in 4% buffered formalin ON at RT.
  • Deparaffinization and rehydration through graded xylene and graded alcohol series:
    • xylene twice for 3min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • 50% ethanol for 1min.
    • H2O for 2min.
  • Boil the sections in the decloaking chamber (Biocare Medical):
    • fill the outer chamber with 500ml distilled water.
    • fill a plastic jar with diluted Antigen unmasking solution (Vector, H3301) diluted 1:100.
    • Boil sections for 8 minutes, turn it off and leave for 1h.
  • Rinse sections with distilled water 3 times.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
  • Block sections in normal goat serum (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:50 for 1h at RT.
  • Blot excess serum from sections; do not rinse.
  • Incubate sections with primary antibody rabbit-anti CSF1R antibody (antibodies-online, ABIN683788, lot 9I02V3) diluted 1:100-1:500 in with 0.05M Tris-HCl, pH7.6 ON at 4°C. Include no primary antibody negative controls.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times. Keep negative controls in a separate jar.
  • Incubate sections with biotinylated secondary antibody anti-rabbit (Vector, Vectastain ABC-Elite kit, PK 6101, lot 300712) diluted 1:200 for 30min at RT.
  • Mix 20µl of reagent A and reagent B of the Vectastain Elite ABC HRP kit (Vectorlabs, PK-6101, lot 300712) in 1ml 0.05M TrisHCl pH7.6 and leave the mixture for 30min at RT.
  • Rinse sections with 0.05M Tris-HCl pH7.6, 3 times.
  • Incubate sections in the ABC reagent mix for 45min at RT.
  • Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
  • DAB staining using peroxidase substrate kit (Vector, product SK-4100, lot 020612):
    • Mix thoroughly 2.5ml and 1 drop buffer stock.
    • Add 2 drops DAB and mix thoroughly.
    • Add 1 drop H2O2 and mix thoroughly.
  • Rinse sections with 0.05M Tris-HCl pH7.6 3 times.
  • Stain sections with Mayer HTX (Histolab, 01820, lot 00811.) for 2-3min at RT.
  • Rinse sections in tap water for 5-10min.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • xylene twice for 2min.
  • Mount sections in Pertex mounting medium (Histolab, 00811).
  • Dry sections in a ventilated place.
  • Image acquisition (x10 and x20 objectives).
    Experimental Notes The blocking step with avidin and biotin was omitted for the no primary antibody negative controls. As a result, there is some unspecific staining in these sections. ABIN683788 worked nicely on the canine lymph node that was used as a positive control.
    Validation Images
    Immunohistochemistry validation image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) (AA 694-744), (pTyr723) antibody (ABIN683788) Staining of canine lymph node tissue with ABIN683788. The primary antibody ABIN683788...