anti-Mouse (Murine) CSF1R antibody for Flow Cytometry

Recommended CSF1R Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
  • MGC89146
  • C-FMS
  • CD115
  • CSF-1R
  • CSFR
  • FIM2
  • FMS
  • HDLS
  • M-CSF-R
  • AI323359
  • Csfmr
  • Fim-2
  • Fms
  • M-CSFR
  • CSF-1-R
  • c-fms
  • mrfms
  • CSF1
  • M-CSF
  • csf1r
  • fms
  • panther
  • colony stimulating factor 1 receptor
  • csf1r protein
  • macrophage colony-stimulating factor receptor
  • colony stimulating factor 1 receptor, a
  • CSF1R
  • csf1r
  • m-csfr
  • Csf1r
  • csf1ra
Reactivity
Mouse (Murine), Rat (Rattus)
344
201
84
23
12
9
8
6
4
3
3
3
2
2
2
1
Host
Rat
232
111
107
32
4
Clonality
Monoclonal
Conjugate
This CSF1R antibody is conjugated to PE
34
27
25
18
18
13
11
9
9
8
8
7
6
6
5
5
5
5
5
5
4
3
3
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
308
227
196
155
84
57
28
16
16
13
8
7
6
5
4
3
3
3
3
2
2
Supplier
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Catalog No. ABIN317385
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.323238 ABIN4899519 CyTOF FACS Rat IgG2b AA 20-511 Log in to see 460615 2
14.323238 ABIN4899518 FACS WB Biotin Sheep IgG AA 20-511 Log in to see Polyclonal 1
14.323238 ABIN2478115 FACS Rat IgG1 Log in to see 604B5 2E11 4
14.323238 ABIN2657307 ELISA (Detection) FACS Alexa Fluor 488 Rat IgG2a kappa Log in to see AFS98 5
14.323238 ABIN2660806 FACS Biotin Rat IgG2a kappa Log in to see AFS98 5
14.323238 ABIN2662953 FACS IF IHC PE Rat IgG2a kappa Log in to see AFS98 7
14.323238 ABIN2658444 CyTOF FACS APC Rat IgG2a kappa Log in to see AFS98 7
14.323238 ABIN2664139 FACS Rat IgG2a kappa Log in to see AFS98 5
14.323238 ABIN4896252 FACS Allophycocyanin Rat IgG2b AA 20-511 Log in to see 460615
14.323238 ABIN4896254 FACS PE Rat IgG2b AA 20-511 Log in to see 460615
14.323238 ABIN4896253 FACS PerCP Rat IgG2b AA 20-511 Log in to see 460615
1 ABIN317383 FACS Rat IgG1 Log in to see 604B5 2E11 1
1 ABIN317381 Func FACS Rat IgG1 Log in to see 604B5 2E11 3
1 ABIN2656811 FACS Alexa Fluor 594 Rat IgG2a kappa Log in to see AFS98 5
1 ABIN475128 FACS Biotin Rat IgG2a, kappa Log in to see AFS98
1 ABIN476391 FACS APC Rat IgG2a, kappa Log in to see AFS98
1 ABIN475308 FACS PE Rat IgG2a, kappa Log in to see AFS98
1 ABIN474868 FACS IHC (fro) WB Rat IgG2a, kappa Log in to see AFS98
1 ABIN2657308 ELISA (Detection) FACS Alexa Fluor 488 Rat IgG2a kappa Log in to see AFS98 5
1 ABIN2660805 FACS Biotin Rat IgG2a kappa Log in to see AFS98 5

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(344), (201), (84), (23), (12), (9), (8), (6), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rat
(232), (111), (107), (32), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF1R antibody is conjugated to PE
(34), (27), (25), (18), (18), (13), (11), (9), (9), (8), (8), (7), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(308), (227), (196), (155), (84), (57), (28), (16), (16), (13), (8), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2)
Pubmed 3 references available
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Product Details anti-CSF1R Antibody

Target Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN317385) Images
Specificity This antibody recognizes the CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.
Cross-Reactivity (Details) Species reactivity (tested):Mouse and Rat.
Characteristics Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor
Purification Purified IgG prepared by ion exchange chromatography
Immunogen RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.
Clone 604B5 2E11
Isotype IgG1

Target Details CSF1R

Product Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R antibody (ABIN317385) Images back to top
Antigen
Alternative Name CD115 (CSF1R Antibody Abstract)
Background CD115, also known as c-fms, macrophage Colony Stimulating Factor Receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms
Gene ID 12978
UniProt P09581
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization

Application Details

Product Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R antibody (ABIN317385) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R antibody (ABIN317385) Images back to top
Reconstitution Restore with 1.0 mL distilled water.
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CSF1R antibody (ABIN317385)

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
Background publications

MacDonald, Rowe, Bofinger, Thomas, Sasmono, Hume, Hill: "The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1399-405, 2005

Shadduck, Fischer, DePasquale, Gilmore: "Paradoxical stimulation of normal and leukemic rat hematopoiesis by monoclonal antibody to CSF-1 receptor." in: Experimental hematology, Vol. 24, Issue 2, pp. 314-7, 1996

Gilmore, Shadduck: "Inhibition of day-12 spleen colony-forming units by a monoclonal antibody to the murine macrophage/monocyte colony-stimulating factor receptor." in: Blood, Vol. 85, Issue 10, pp. 2731-4, 1995

Images

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN317385) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Colony Stimulating Factor 1 Receptor (CSF1R) antibody (PE) (ABIN317385) anti-Colony Stimulating Factor 1 Receptor (CSF1R) antibody (PE)