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anti-Rat (Rattus) CSF1R antibody for Flow Cytometry

Recommended CSF1R Antibody (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
  • AI323359
  • c-fms
  • C-FMS
  • CD115
  • CSF-1-R
  • CSF-1R
  • CSF1
  • csf1r
  • Csfmr
  • CSFR
  • Fim-2
  • FIM2
  • fms
  • FMS
  • Fms
  • HDLS
  • M-CSF
  • M-CSF-R
  • M-CSFR
  • MGC89146
  • mrfms
  • panther
Reactivity
Mouse (Murine), Rat (Rattus)
234
158
56
21
12
9
7
4
4
4
2
2
2
2
1
Host
Rat
227
79
26
22
3
Clonality
Monoclonal
Conjugate
This CSF1R antibody is conjugated to PE
27
24
21
18
16
8
4
4
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
223
182
94
86
37
23
12
12
11
10
4
4
3
3
3
2
2
2
2
1
Supplier
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Catalog No. ABIN317385
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010665731 ABIN317383 FACS Rat IgG1 Log in to see 604B5 2E11 1
0.0010665731 ABIN317381 Func FACS Rat IgG1 Log in to see 604B5 2E11 3

Top referenced anti-CSF1R antibody for Flow Cytometry

Similar anti-CSF1R Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 80 Antibodies 36 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Flow Cytometry (ICFC) 1 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 41 Antibodies 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 12 Antibodies 7 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 1 Antibodies
Immunochromatography (IC) 2 Antibodies 1 Antibodies

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(234), (158), (56), (21), (12), (9), (7), (4), (4), (4), (2), (2), (2), (2), (1)
Host Rat
(227), (79), (26), (22), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CSF1R antibody is conjugated to PE
(27), (24), (21), (18), (16), (8), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(223), (182), (94), (86), (37), (23), (12), (12), (11), (10), (4), (4), (3), (3), (3), (2), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-CSF1R Antibody

Target Details CSF1R Application Details Handling References for anti-CSF1R Antibody (ABIN317385) Images
Specificity This antibody recognizes the CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.
Cross-Reactivity (Details) Species reactivity (tested):Mouse and Rat.
Characteristics Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor
Purification Purified IgG prepared by ion exchange chromatography
Immunogen RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.
Clone 604B5 2E11
Isotype IgG1

Target Details CSF1R

Product Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R Antibody (ABIN317385) Images back to top
Antigen
Alternative Name CD115 (CSF1R Antibody Abstract)
Background CD115, also known as c-fms, macrophage Colony Stimulating Factor Receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms
Gene ID 12978
UniProt P09581
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling

Application Details

Product Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R Antibody (ABIN317385) Images back to top
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R Antibody (ABIN317385) Images back to top
Reconstitution Restore with 1.0 mL distilled water.
Buffer PBS, 0.09 % Sodium Azide, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CSF1R Antibody (ABIN317385)

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
Background publications

MacDonald, Rowe, Bofinger, Thomas, Sasmono, Hume, Hill: "The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1399-405, 2005

Shadduck, Fischer, DePasquale, Gilmore: "Paradoxical stimulation of normal and leukemic rat hematopoiesis by monoclonal antibody to CSF-1 receptor." in: Experimental hematology, Vol. 24, Issue 2, pp. 314-7, 1996

Gilmore, Shadduck: "Inhibition of day-12 spleen colony-forming units by a monoclonal antibody to the murine macrophage/monocyte colony-stimulating factor receptor." in: Blood, Vol. 85, Issue 10, pp. 2731-4, 1995

Images

Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling References for anti-CSF1R Antibody (ABIN317385) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CSF1R antibody (Colony Stimulating Factor 1 Receptor)  (PE) (ABIN317385) anti-Colony Stimulating Factor 1 Receptor (CSF1R) antibody (PE)