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|Antigen||Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CSF1R antibody is conjugated to PE Alternatives|
Flow Cytometry (FACS)
|3 references available|
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Product Details anti-CSF1R AntibodyTarget Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN317385) Images
|Specificity||This antibody recognizes the CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.|
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse and Rat.|
|Characteristics||Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.|
Target Details CSF1RProduct Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R antibody (ABIN317385) Images back to top
|Alternative Name||CD115 (CSF1R Antibody Abstract)|
|Background||CD115, also known as c-fms, macrophage Colony Stimulating Factor Receptor (M-CSFR), or CSF-1R, exists in immature (gp130c-fms) and mature (gp150c-fms) forms. CSF-1R exhibits an intrinsic tyrosine-specific protein kinase activity. It is expressed on the cells of the mononuclear phagocyte lineage. CSF-1R is an integral transmembrane glycoprotein consisting of a 512 amino acid extracellular domain that includes the CSF-1 binding site, a single 25 amino acid membrane-spanning segment, and a 435 amino acid cytoplasmic portion that includes the tyrosine kinase domain. CD115 is expressed by cells of the monocytic lineage and by progenitor cells.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms|
|Research Area||CD Antigens, Surface Receptors of Immune Cells|
|Pathways||RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R antibody (ABIN317385) Images back to top
Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R antibody (ABIN317385) Images back to top
|Reconstitution||Restore with 1.0 mL distilled water.|
|Buffer||PBS, 0.09 % Sodium Azide, 1 % BSA|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CSF1R antibody (ABIN317385)Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
MacDonald, Rowe, Bofinger, Thomas, Sasmono, Hume, Hill: "The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1399-405, 2005
Shadduck, Fischer, DePasquale, Gilmore: "Paradoxical stimulation of normal and leukemic rat hematopoiesis by monoclonal antibody to CSF-1 receptor." in: Experimental hematology, Vol. 24, Issue 2, pp. 314-7, 1996
Gilmore, Shadduck: "Inhibition of day-12 spleen colony-forming units by a monoclonal antibody to the murine macrophage/monocyte colony-stimulating factor receptor." in: Blood, Vol. 85, Issue 10, pp. 2731-4, 1995
ImagesProduct Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN317385) back to top