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Human CSF1R ELISA Kit

Recommended CSF1R Product (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) ELISA Kits
  • AI323359
  • c-fms
  • C-FMS
  • CD115
  • CSF-1-R
  • CSF-1R
  • CSF1
  • csf1r
  • Csfmr
  • CSFR
  • Fim-2
  • FIM2
  • fms
  • FMS
  • Fms
  • HDLS
  • M-CSF
  • M-CSF-R
  • M-CSFR
  • MGC89146
  • mrfms
  • panther
Epitope
AA 20-512
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
17
11
6
Methode Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1672804
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN1379981 Sandwich ELISA Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.30628014 ABIN2708113 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN1380305 Cell ELISA Log in to see
0.0012155735 ABIN1380394 Cell ELISA Log in to see
0.0012155735 ABIN1381056 Cell ELISA Log in to see
0.0012155735 ABIN2042415 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012155735 ABIN2114492 Cell Lysate, Serum, Plasma 63-4000 pg/mL 63 pg/mL Log in to see
0.0012155735 ABIN2113228 Cell ELISA Log in to see
0.0012155735 ABIN2113229 Cell ELISA Log in to see
0.0012155735 ABIN2113227 Cell ELISA Log in to see
0.0012155735 ABIN2683922 Sandwich ELISA Cell Lysate, Cell Culture Supernatant, Plasma, Serum Log in to see
0.0012155735 ABIN2683923 Cell ELISA Cell Samples, Suspension Cell Culture Log in to see
0.0012155735 ABIN2536292 Serum, Plasma, Biological Fluids 80-4000 pg/mL 80 pg/mL Log in to see
0.0012155735 ABIN625336 Sandwich ELISA Log in to see
0.0012155735 ABIN455772 Log in to see
0.0012155735 ABIN2212633 Log in to see
0.0012155735 ABIN2949266 Log in to see

Top referenced CSF1R ELISA Kit for ELISA

Similar CSF1R ELISA Kits

Application / Reactivity Human
ELISA 18 ELISA Kits

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) ELISA Kits
Epitope AA 20-512
Reactivity Human
Kits with alternative reactivity to:
(17), (11), (6)
Methode Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CSF1R ELISA Kit

Target details Application Details Handling References for CSF1R Kit (ABIN1672804) Images
Purpose For quantitative detection of human CSF1R in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human CSF1R
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human CSF1R antibod - 1
  • Lyophilized recombinant human CSF1R standar - 10ng/tubex2
  • Biotinylated anti- human CSF1R antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: I20-E512

Target details

Product Details CSF1R ELISA Kit Application Details Handling References for CSF1R Kit (ABIN1672804) Images back to top
Antigen
Alternative Name CSF1R (CSF1R ELISA Kit Abstract)
Background CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5(5q32) on the Crick(minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
Gene ID 1436
UniProt P07333
Pathways RTK Signaling

Application Details

Product Details CSF1R ELISA Kit Target details Handling References for CSF1R Kit (ABIN1672804) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human CSF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CSF1R has been precoated onto 96-well plates. Standards(NSO, I20-E512) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CSF1R amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CSF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CSF1R standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 477, Standard deviation: 27.7, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 1541, Standard deviation: 92.46, CV(%): 6
  • Sample 3: n=16, Mean(pg/ml): 2626, Standard deviation: 128.7, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 527, Standard deviation: 32.15, CV(%): 6.1
  • Sample 2: n=24, Mean(pg/ml): 1712, Standard deviation: 114.7, CV(%): 6.7
  • Sample 3: n=24, Mean(pg/ml): 3015, Standard deviation: 168.84, CV(%): 5.6
Restrictions For Research Use only

Handling

Product Details CSF1R ELISA Kit Target details Application Details References for CSF1R Kit (ABIN1672804) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CSF1R Kit (ABIN1672804)

Product Details CSF1R ELISA Kit Target details Application Details Handling Images back to top
Background publications

Pollard, Hennighausen: "Colony stimulating factor 1 is required for mammary gland development during pregnancy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 20, pp. 9312-6, 1994

Galland, Stefanova, Lafage, Birnbaum: "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23." in: Cytogenetics and cell genetics, Vol. 60, Issue 2, pp. 114-6, 1992

Images

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Supplier Images
ELISA image for CSF1R ELISA Kit (Colony Stimulating Factor 1 Receptor) (ABIN1672804) Human CSF1R/M-CSFR PicoKine ELISA Kit standard curve