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Mouse (Murine) CSF1R ELISA Kit

Recommended CSF1R Product (supplied by: Log in to see )

Antigen
Colony Stimulating Factor 1 Receptor (CSF1R) ELISA Kits
  • MGC89146
  • C-FMS
  • CD115
  • CSF-1R
  • CSFR
  • FIM2
  • FMS
  • HDLS
  • M-CSF-R
  • AI323359
  • Csfmr
  • Fim-2
  • Fms
  • M-CSFR
  • CSF-1-R
  • c-fms
  • mrfms
  • CSF1
  • M-CSF
  • csf1r
  • fms
  • panther
  • colony stimulating factor 1 receptor
  • csf1r protein
  • macrophage colony-stimulating factor receptor
  • colony stimulating factor 1 receptor, a
  • CSF1R
  • csf1r
  • m-csfr
  • Csf1r
  • csf1ra
Epitope
AA 20-511
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
10
6
Method Type
Sandwich ELISA
Detection Range
62.5-4000 pg/mL
Minimum Detection Limit
62.5 pg/mL
Application
ELISA
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Catalog No. ABIN1672805
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.891415 ABIN2708440 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN813144 Sandwich ELISA Serum, Plasma, Tissue Homogenate 10-0.16 ng/mL 10 ng/mL Log in to see
1 ABIN1380394 Cell ELISA Log in to see
1 ABIN1380305 Cell ELISA Log in to see
1 ABIN2683924 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2042416 Sandwich ELISA 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2113228 Cell ELISA Log in to see
1 ABIN2113229 Cell ELISA Log in to see
1 ABIN1141367 Log in to see
1 ABIN2949267 Log in to see

Similar CSF1R ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 11 ELISA Kits

General

Antigen Colony Stimulating Factor 1 Receptor (CSF1R) ELISA Kits
Epitope AA 20-511
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (10), (6)
Method Type Sandwich ELISA
Detection Range 62.5-4000 pg/mL
Minimum Detection Limit 62.5 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details CSF1R ELISA Kit

Target details Application Details Handling References for CSF1R Kit (ABIN1672805) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CSF1R/M-CSFR
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: A20-S511
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A20-S511

Target details

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Antigen
Alternative Name CSF1R (CSF1R ELISA Kit Abstract)
Background

Protein Function: Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP- 1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor. .

Background: CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5(5q32) on the Crick(minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Synonyms: Macrophage colony-stimulating factor 1 receptor,CSF-1 receptor,CSF-1-R,CSF-1R,M-CSF-R,2.7.10.1,Proto-oncogene c-Fms,CD115,Csf1r,Csfmr, Fms,

Full Gene Name: Macrophage colony-stimulating factor 1 receptor

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . The autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
Gene ID 12978
UniProt P09581
Pathways RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Tissue Specificity: Widely expressed. .

Plate Pre-coated,Strips (12 x 8)
Protocol mouse CSF1R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CSF1R has been precoated onto 96-well plates. Standards(NSO, A20-S511) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CSF1R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CSF1R amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CSF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CSF1R standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 379, Standard deviation: 20.85, CV(%): 5.5
  • Sample 2: n=16, Mean(pg/ml): 1361, Standard deviation: 62.61, CV(%): 4.6
  • Sample 3: n=16, Mean(pg/ml): 2468, Standard deviation: 155.5, CV(%): 6.3,
  • Sample 1: n=24, Mean(pg/ml): 413, Standard deviation: 28.5, CV(%): 6.9
  • Sample 2: n=24, Mean(pg/ml): 1533, Standard deviation: 89, CV(%): 5.8
  • Sample 3: n=24, Mean(pg/ml): 2766, Standard deviation: 202, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details CSF1R ELISA Kit Target details Application Details References for CSF1R Kit (ABIN1672805) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for CSF1R Kit (ABIN1672805)

Product Details CSF1R ELISA Kit Target details Application Details Handling Images back to top
Background publications

Pollard, Hennighausen: "Colony stimulating factor 1 is required for mammary gland development during pregnancy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 20, pp. 9312-6, 1994

Galland, Stefanova, Lafage, Birnbaum: "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23." in: Cytogenetics and cell genetics, Vol. 60, Issue 2, pp. 114-6, 1992

Images

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Supplier Images
ELISA image for CSF1R ELISA Kit (Colony Stimulating Factor 1 Receptor) (ABIN1672805) Mouse CSF1R/M-CSFR PicoKine ELISA Kit standard curve