We are preparing the requested document. Please wait, this may take a while...!
|Antigen||Discoidin Domain Receptor tyrosine Kinase 2 (DDR2) Antibodies|
|Epitope||AA 1-399 Alternatives|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This DDR2 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
|2 references available|
|Supplier||Log in to see|
Product Details anti-DDR2 AntibodyTarget Details DDR2 Application Details Handling ProductDetails: References for anti-DDR2 antibody (ABIN2001101) Images
|Characteristics||Produced in rabbits immunized with purified, recombinant Mouse DDR2 (rM DDR2, ABIN2007949 NP_072075.2, Met 1-Arg 399,. DDR2 specific IgG was purified by Mouse DDR2 affinity chromatography.|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Mouse DDR2 protein (ABIN2007949)|
Target Details DDR2Product Details anti-DDR2 Antibody Application Details Handling ProductDetails: References for anti-DDR2 antibody (ABIN2001101) Images back to top
|Alternative Name||DDR2 (DDR2 Antibody Abstract)|
|Background||Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple- helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.|
|Research Area||Cardiovascular, Cardiogenesis|
Application DetailsProduct Details anti-DDR2 Antibody Target Details DDR2 Handling ProductDetails: References for anti-DDR2 antibody (ABIN2001101) Images back to top
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse DDR2. The detection limit for Mouse DDR2 is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-DDR2 Antibody Target Details DDR2 Application Details ProductDetails: References for anti-DDR2 antibody (ABIN2001101) Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
ProductDetails: References for anti-DDR2 antibody (ABIN2001101)Product Details anti-DDR2 Antibody Target Details DDR2 Application Details Handling Images back to top
Zhang, Ding, Zhang, Su, Li, Liu, Ma, Yao: "Expression of discoidin domain receptor 2 (DDR2) extracellular domain in pichia pastoris and functional analysis in synovial fibroblasts and NIT3T3 cells." in: Molecular and cellular biochemistry, Vol. 290, Issue 1-2, pp. 43-53, 2006
Leitinger, Steplewski, Fertala: "The D2 period of collagen II contains a specific binding site for the human discoidin domain receptor, DDR2." in: Journal of molecular biology, Vol. 344, Issue 4, pp. 993-1003, 2004
ImagesProduct Details anti-DDR2 Antibody Target Details DDR2 Application Details Handling ProductDetails: References for anti-DDR2 antibody (ABIN2001101) back to top