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anti-Human EPH Receptor A2 antibody for Flow Cytometry

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
Epitope
AA 25-534
28
20
19
18
18
17
15
15
14
13
12
10
9
7
7
7
6
6
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
264
159
56
22
14
10
8
7
4
4
2
2
2
2
1
Host
Mouse
197
92
23
2
1
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
17
12
11
10
9
8
6
6
6
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
211
145
90
59
54
27
17
9
6
6
4
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN4899912
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.2075615 ABIN4899913 FACS IHC WB Goat IgG AA 25-534 Log in to see Polyclonal 3
14.2075615 ABIN1687673 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP169
14.2075615 ABIN179724 cELISA FACS IF Mouse IgG1 Log in to see Kalpha-5H5 4
14.2075615 ABIN4308656 FACS Alexa Fluor 647 Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN2662716 FACS PE Mouse IgG2b kappa Log in to see SHM16 5
14.2075615 ABIN2664953 FACS ICFC Mouse IgG2b kappa Log in to see SHM16 5
14.2075615 ABIN4896887 FACS Allophycocyanin Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN4896891 FACS PE Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN4896889 FACS Alexa Fluor 488 Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN4896890 FACS Alexa Fluor 700 Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN4896888 FACS PerCP Mouse IgG2a AA 25-534 Log in to see 371805
14.2075615 ABIN4899911 FACS WB Biotin Goat IgG AA 25-534 Log in to see Polyclonal
1 ABIN391885 FACS IHC (p) WB Rabbit Ig AA 30-60, N-Term Log in to see Polyclonal 1
1 ABIN488132 EIA FACS IF Mouse IgG1 Log in to see GM5H5 4
1 ABIN1449314 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP169
1 ABIN4308660 FACS Alexa Fluor 405 Mouse IgG2a AA 25-534 Log in to see 371805
1 ABIN4308659 FACS Alexa Fluor 350 Mouse IgG2a AA 25-534 Log in to see 371805
1 ABIN4308657 FACS Alexa Fluor 750 Mouse IgG2a AA 25-534 Log in to see 371805
1 ABIN4308658 FACS Alexa Fluor 594 Mouse IgG2a AA 25-534 Log in to see 371805
1 ABIN2662717 FACS PE Mouse IgG2b kappa Log in to see SHM16 5

Top referenced anti-EPH Receptor A2 antibody for Flow Cytometry

Similar anti-EPH Receptor A2 Antibodies

Application / Reactivity Human
Cell-ELISA (cELISA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 17 Antibodies
ELISA 127 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 64 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 59 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 55 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 191 Antibodies

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope AA 25-534
(28), (20), (19), (18), (18), (17), (15), (15), (14), (13), (12), (10), (9), (7), (7), (7), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(264), (159), (56), (22), (14), (10), (8), (7), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(197), (92), (23), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor A2 antibody is un-conjugated
(17), (12), (11), (10), (9), (8), (6), (6), (6), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(211), (145), (90), (59), (54), (27), (17), (9), (6), (6), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images
Specificity Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317
Clone 371805
Isotype IgG2a

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Antigen
Alternative Name EphA2 (EPHA2 Antibody Abstract)
Background Alternative Names: ARCC2, EC 2.7.10, EC 2.7.10.1, Eck, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, EphA2, ephrin type-A receptor 2, Epithelial cell kinase, Myk2, Sek2, soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK
Gene ID 1969
UniProt P29317
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor A2 Antibody (ABIN4899912)

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling Images back to top
Product cited in:

Liao-Chan, Daine-Matsuoka, Heald, Wong, Lin, Cai, Lai, DAlessio, Theunissen: "Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124708, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Park, Park, Kim, Pyo, Kimura, Sathirachinda, Choy, Min, Gambhir, Hong: "Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132976, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Yeast).

de Cárcer, Escobar, Higuero, García, Ansón, Pérez, Mollejo, Manning, Meléndez, Abad-Rodríguez, Malumbres: "Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1225-39, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 25-534) (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 25-534) (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...