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|Application / Reactivity||Human|
|Cell-ELISA (cELISA)||1 Antibodies|
|Dot Blot (DB)||18 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||23 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||17 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||41 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Western Blotting (WB)||151 Antibodies|
|Antigen||EPH Receptor A2 (EPHA2) Antibodies|
|Epitope||Internal Region Alternatives|
|Conjugate||This EPH Receptor A2 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-EPH Receptor A2 AntibodyTarget Details EPH Receptor A2 Application Details Handling Images
|Predicted Reactivity||Monkey,Mouse (Murine)|
|Characteristics||Cellular Localization: Membrane|
|Purification||Purified by Protein A/G|
|Immunogen||Synthetic peptide derived from the internal region of human EphA2 protein.|
Target Details EPH Receptor A2Product Details anti-EPH Receptor A2 Antibody Application Details Handling Images back to top
|Alternative Name||EphA2 (EPHA2 Antibody Abstract)|
|Background||Ephrin type-A receptor 2 (EphA2) is a protein-tyrosine kinase in the ephrin receptor subfamily. EphA2 plays a role for angiogenesis and tumor neovascularization. High levels of EphA2 protein expression can be detected in some tumor cells such as breast carcinoma, bladder carcinoma, renal cell carcinoma (RCC), and non-small cell lung carcinoma (NSCLC). Inhibition of EphA2 kinase activity is considered for the treatment of various tumors such as RCC and NSCLC.|
|Molecular Weight||135 kDa|
Application DetailsProduct Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling Images back to top
|Application Notes||IHC Dilution: 1:100|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Images back to top
|Buffer||rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
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