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anti-Human EPH Receptor A2 antibody for Flow Cytometry

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • ARCC2
  • AW545284
  • CTPA
  • CTPP1
  • CTRCT6
  • Eck
  • ECK
  • epha2
  • EPHA2
  • MGC89505
  • Myk2
  • Sek-2
  • Sek2
Epitope
Internal Region
27
20
19
18
18
16
15
13
13
11
9
7
7
6
6
6
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
247
111
45
10
6
5
4
4
4
2
2
2
1
Host
Rabbit
188
80
14
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
13
10
10
10
8
6
6
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
211
148
58
53
42
23
18
17
6
6
4
4
2
1
1
1
Supplier
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Catalog No. ABIN1687673
$ 648.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN179724 cELISA FACS IF Mouse IgG1 Log in to see Kalpha-5H5 4
0.30226976 ABIN2662716 FACS PE Mouse IgG2b kappa Log in to see SHM16 5
0.30226976 ABIN2664953 FACS ICFC Mouse IgG2b kappa Log in to see SHM16 5
0.30226976 ABIN2160947 FACS WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.0010482287 ABIN391885 FACS IHC (p) WB Rabbit Ig AA 30-60, N-Term Log in to see Polyclonal 1
0.0010482287 ABIN488132 EIA FACS IF Mouse IgG1 Log in to see GM5H5 4
0.0010482287 ABIN1449313 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP169
0.0010482287 ABIN204192 ICC FACS WB Mouse IgG2a Extracellular Domain Log in to see
0.0010482287 ABIN594813 FACS PE Mouse IgG1 Log in to see D7
0.0010482287 ABIN1976517 FACS IHC ELISA WB PE Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN1963136 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN2662717 FACS PE Mouse IgG2b kappa Log in to see SHM16 5
0.0010482287 ABIN1965513 FACS IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN1969586 FACS IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN1971922 FACS IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN1974181 FACS IHC ELISA WB HRP Rabbit IgG N-Term Log in to see Polyclonal
0.0010482287 ABIN2664954 FACS ICFC Mouse IgG2b kappa Log in to see SHM16 5
0.0010482287 ABIN1978828 FACS IHC (p) Rabbit IgG Internal Region Log in to see SP169
0.0010482287 ABIN4308656 FACS Alexa Fluor 647 Mouse IgG2a AA 25-534 Log in to see 371805
0.0010482287 ABIN4308657 FACS Alexa Fluor 750 Mouse IgG2a AA 25-534 Log in to see 371805

Top referenced anti-EPH Receptor A2 antibody for Flow Cytometry

Similar anti-EPH Receptor A2 Antibodies

Application / Reactivity Human
Cell-ELISA (cELISA) 1 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 131 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 48 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 193 Antibodies

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope Internal Region
(27), (20), (19), (18), (18), (16), (15), (13), (13), (11), (9), (7), (7), (6), (6), (6), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(247), (111), (45), (10), (6), (5), (4), (4), (4), (2), (2), (2), (1)
Host Rabbit
(188), (80), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor A2 antibody is un-conjugated
(13), (10), (10), (10), (8), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(211), (148), (58), (53), (42), (23), (18), (17), (6), (6), (4), (4), (2), (1), (1), (1)
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling Images
Predicted Reactivity Monkey,Mouse (Murine)
Characteristics Cellular Localization: Membrane
Purification Purified by Protein A/G
Immunogen Synthetic peptide derived from the internal region of human EphA2 protein.
Clone SP169
Isotype IgG

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EphA2 (EPHA2 Antibody Abstract)
Background Ephrin type-A receptor 2 (EphA2) is a protein-tyrosine kinase in the ephrin receptor subfamily. EphA2 plays a role for angiogenesis and tumor neovascularization. High levels of EphA2 protein expression can be detected in some tumor cells such as breast carcinoma, bladder carcinoma, renal cell carcinoma (RCC), and non-small cell lung carcinoma (NSCLC). Inhibition of EphA2 kinase activity is considered for the treatment of various tumors such as RCC and NSCLC.
Molecular Weight 135 kDa
Gene ID 1969
Research Area Signaling
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling Images back to top
Application Notes IHC Dilution: 1:100
Comment

Positive Control:
IHC (p): Breast Carcinoma
FC: A431 Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) Flow cytometric analysis of rabbit anti-EphA2 (SP169) antibody in A431 (green) compar...
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-EPH Receptor A2 antibody (EPHA2) (Internal Region) (ABIN1687673) anti-EPH Receptor A2 (EPHA2) (Internal Region) antibody (Image 7)