anti-Human EPH Receptor A2 antibody for Immunocytochemistry

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
Epitope
AA 25-534
28
27
26
25
21
20
18
15
15
14
13
11
9
8
7
7
6
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
298
181
56
22
14
10
8
7
4
4
2
2
2
2
1
Host
Mouse
218
102
45
4
1
1
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
21
15
14
13
13
11
9
8
8
8
8
8
8
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
251
164
119
68
57
27
17
11
8
7
6
4
3
2
2
1
1
1
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Catalog No. ABIN4899912
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN204192 ICC FACS WB Mouse IgG2a Extracellular Domain Log in to see
1 ABIN211915 Func ICC IP WB Mouse IgG1 Log in to see
1 ABIN5646025 CyTOF FACS ICC WB Mouse IgG2a AA 25-534 Log in to see 371805

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope AA 25-534
(28), (27), (26), (25), (21), (20), (18), (15), (15), (14), (13), (11), (9), (8), (7), (7), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(298), (181), (56), (22), (14), (10), (8), (7), (4), (4), (2), (2), (2), (2), (1)
Host Mouse
(218), (102), (45), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor A2 antibody is un-conjugated
(21), (15), (14), (13), (13), (11), (9), (8), (8), (8), (8), (8), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(251), (164), (119), (68), (57), (27), (17), (11), (8), (7), (6), (4), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 antibody (ABIN4899912) Images
Specificity Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317
Clone 371805
Isotype IgG2a

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling References for anti-EPH Receptor A2 antibody (ABIN4899912) Images back to top
Antigen
Alternative Name EphA2 (EPHA2 Antibody Abstract)
Background Alternative Names: ARCC2, EC 2.7.10, EC 2.7.10.1, Eck, ECKepithelial cell receptor protein tyrosine kinase, EPH receptor A2, EphA2, ephrin type-A receptor 2, Epithelial cell kinase, Myk2, Sek2, soluble EPHA2 variant 1, Tyrosine-protein kinase receptor ECK
Gene ID 1969
UniProt P29317
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling References for anti-EPH Receptor A2 antibody (ABIN4899912) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details References for anti-EPH Receptor A2 antibody (ABIN4899912) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor A2 antibody (ABIN4899912)

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling Images back to top
Product cited in:

Liao-Chan, Daine-Matsuoka, Heald, Wong, Lin, Cai, Lai, DAlessio, Theunissen: "Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124708, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Park, Park, Kim, Pyo, Kimura, Sathirachinda, Choy, Min, Gambhir, Hong: "Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132976, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Yeast).

de Cárcer, Escobar, Higuero, García, Ansón, Pérez, Mollejo, Manning, Meléndez, Abad-Rodríguez, Malumbres: "Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1225-39, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 antibody (ABIN4899912) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 25-534) antibody (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...