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anti-Human EPH Receptor A2 antibody for Immunocytochemistry

Recommended EPH Receptor A2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A2 (EPHA2) Antibodies
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
Epitope
AA 25-534
28
20
19
18
18
17
15
13
13
13
12
11
7
7
6
6
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
261
118
45
10
7
5
4
4
4
2
2
2
1
Host
Mouse
194
87
17
2
Clonality
Monoclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
15
11
10
10
8
6
6
6
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
213
149
68
60
55
23
18
17
6
6
4
4
2
2
1
1
1
Supplier
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Catalog No. ABIN4899912
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN204192 ICC FACS WB Mouse IgG2a Extracellular Domain Log in to see
1 ABIN211915 Func ICC IP WB Mouse IgG1 Log in to see
1 ABIN2972585 ICC IF WB Rabbit Center, pTyr588, pTyr596 Log in to see Polyclonal
1 ABIN2972587 ICC IF WB Rabbit Center Log in to see Polyclonal

Top referenced anti-EPH Receptor A2 antibody for Immunocytochemistry

Similar anti-EPH Receptor A2 Antibodies

Application / Reactivity Human
Cell-ELISA (cELISA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 131 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Flow Cytometry (FACS) 55 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 60 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 194 Antibodies

General

Antigen EPH Receptor A2 (EPHA2) Antibodies
Epitope AA 25-534
(28), (20), (19), (18), (18), (17), (15), (13), (13), (13), (12), (11), (7), (7), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(261), (118), (45), (10), (7), (5), (4), (4), (4), (2), (2), (2), (1)
Host Mouse
(194), (87), (17), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This EPH Receptor A2 antibody is un-conjugated
(15), (11), (10), (10), (8), (6), (6), (6), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(213), (149), (68), (60), (55), (23), (18), (17), (6), (6), (4), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-EPH Receptor A2 Antibody

Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images
Specificity Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse EphA4, A5, A6, A7, A8, or recombinant rat EphB1 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EphA2, Gln25-Asn534, Accession # P29317
Clone 371805
Isotype IgG2a

Target Details EPH Receptor A2

Product Details anti-EPH Receptor A2 Antibody Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Antigen
Alternative Name EphA2 (EPHA2 Antibody Abstract)
Gene ID 1969
UniProt P29317
Research Area Signaling
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Application Notes Western Blot 1 μg/mL, Flow Cytometry 0.25 μg/10^6 cells, Immunocytochemistry 8-25 μg/mL, CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details References for anti-EPH Receptor A2 Antibody (ABIN4899912) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer 0.2 m filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C/-20 °C/-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 6 months, -20 to -70 C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor A2 Antibody (ABIN4899912)

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling Images back to top
Product cited in:

Liao-Chan, Daine-Matsuoka, Heald, Wong, Lin, Cai, Lai, DAlessio, Theunissen: "Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124708, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Park, Park, Kim, Pyo, Kimura, Sathirachinda, Choy, Min, Gambhir, Hong: "Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132976, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Yeast).

de Cárcer, Escobar, Higuero, García, Ansón, Pérez, Mollejo, Manning, Meléndez, Abad-Rodríguez, Malumbres: "Plk5, a polo box domain-only protein with specific roles in neuron differentiation and glioblastoma suppression." in: Molecular and cellular biology, Vol. 31, Issue 6, pp. 1225-39, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-EPH Receptor A2 Antibody Target Details EPH Receptor A2 Application Details Handling References for anti-EPH Receptor A2 Antibody (ABIN4899912) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 25-534) (ABIN4899912) EphA2 in A431 Human Cell Line. EphA2 was detected in immersion fixed A431 human epith...
Flow Cytometry (FACS) image for anti-EPH Receptor A2 antibody (EPHA2) (AA 25-534) (ABIN4899912) Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial c...