anti-Human EPH Receptor A4 antibody for Immunofluorescence

Recommended EPH Receptor A4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A4 (EPHA4) Antibodies
  • ephe
  • EPHA4
  • Ci-Eph1
  • sek
  • hek8
  • rtk1
  • tyr01
  • 2900005C20Rik
  • AI385584
  • Cek8
  • Hek8
  • Sek
  • Sek1
  • Tyro1
  • rb
  • HEK8
  • SEK
  • TYRO1
  • RGD1560587
  • EPH receptor A4
  • ephrin receptor epsilon
  • Eph receptor A4
  • EPHA4
  • ephe
  • epha4
  • Epha4
Epitope
AA 40-70, N-Term
30
21
19
15
13
12
10
10
7
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
157
73
37
10
10
10
7
7
7
6
4
4
2
2
2
2
2
1
1
1
Host
Rabbit
150
23
17
2
Clonality
Polyclonal
Conjugate
This EPH Receptor A4 antibody is un-conjugated
9
8
8
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
133
85
85
50
18
16
13
7
4
2
1
1
Options
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Catalog No. ABIN391889
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.845919 ABIN967994 IF IP IHC WB Mouse IgG1 AA 279-472 Log in to see 35-EphA4 3
1 ABIN359794 EIA IF IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 4
1 ABIN1963141 ELISA FACS ICC IF IHC WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1965518 ELISA FACS ICC IF IHC WB APC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1971927 ELISA FACS ICC IF IHC WB FITC Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1976522 ELISA FACS ICC IF IHC WB PE Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1974186 ELISA FACS ICC IF IHC WB HRP Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2600291 ELISA FACS IF IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5536633 FACS IF IHC (p) WB Rabbit Ig Fraction AA 40-70, N-Term Log in to see Polyclonal
1 ABIN1969591 ELISA FACS ICC IF IHC WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2260674 FACS IF IHC ELISA WB APC Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN2260672 FACS IF IHC ELISA WB Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN2260676 FACS IF IHC ELISA WB FITC Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN2260678 FACS IF IHC ELISA WB PE Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN2260673 FACS IF IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN2260675 FACS IF IHC ELISA WB Biotin Rabbit IgG AA 40-70, N-Term Log in to see Polyclonal
1 ABIN1804916 FACS IF IHC (p) WB Rabbit AA 40-70 Log in to see Polyclonal
1 ABIN2793102 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal

General

Antigen EPH Receptor A4 (EPHA4) Antibodies
Epitope AA 40-70, N-Term
(30), (21), (19), (15), (13), (12), (10), (10), (7), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(157), (73), (37), (10), (10), (10), (7), (7), (7), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(150), (23), (17), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EPH Receptor A4 antibody is un-conjugated
(9), (8), (8), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(133), (85), (85), (50), (18), (16), (13), (7), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-EPH Receptor A4 Antibody

Target Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN391889) Images
Predicted Reactivity Chicken,Mouse (Murine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 AA from the N-terminal region of human EphA4.
Clone RB1587
Isotype Ig

Target Details EPH Receptor A4

Product Details anti-EPH Receptor A4 Antibody Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN391889) Images back to top
Antigen
Alternative Name EphA4 (EPHA4 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Synonyms: Ephrin type-A receptor 4 , Tyrosine-protein kinase receptor SEK, Receptor protein-tyrosine kinase HEK8
Molecular Weight 109859 Da
Gene ID 2043
UniProt P54764
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Handling References for anti-EPH Receptor A4 antibody (ABIN391889) Images back to top
Application Notes Suggested dilutions:
WB = 1:1000, IF = 1:100, FACS = 1:10-50, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details References for anti-EPH Receptor A4 antibody (ABIN391889) Images back to top
Format Liquid
Concentration 1.11 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-EPH Receptor A4 antibody (ABIN391889)

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling Images back to top
Background publications

Prevost, Woulfe, Tanaka, Brass: "Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9219-24, 2002

Images

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN391889) back to top
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN391889) Western blot analysis of anti-EphA4 N-term Pab (ABIN391889) in HeLa cell lysate. EphA...
Western Blotting (WB) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN391889) Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term) (ABIN391889). 29...
Immunofluorescence (IF) image for anti-EPH Receptor A4 (EPHA4) (AA 40-70), (N-Term) antibody (ABIN391889) Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endot...