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anti-Human EPH Receptor A4 antibody for Western Blotting

Recommended EPH Receptor A4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A4 (EPHA4) Antibodies
  • 2900005C20Rik
  • AI385584
  • Cek8
  • Ci-Eph1
  • EPHA4
  • ephe
  • HEK8
  • Hek8
  • hek8
  • rb
  • RGD1560587
  • rtk1
  • SEK
  • Sek
  • sek
  • Sek1
  • tyr01
  • Tyro1
  • TYRO1
Epitope
AA 40-70, N-Term
30
21
20
14
13
11
8
8
6
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
141
46
31
5
5
4
3
3
3
2
2
2
2
2
2
1
Host
Rabbit
139
19
3
2
Clonality
Polyclonal
Conjugate
This EPH Receptor A4 antibody is un-conjugated
7
7
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
109
76
67
31
15
11
11
6
2
2
1
1
Supplier
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Catalog No. ABIN391889
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN657836 IHC (p) WB Rabbit Ig AA 464-492, Center Log in to see Polyclonal 2
0.13341247 ABIN359794 EIA IF IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal 4
0.13341247 ABIN359795 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.13341247 ABIN391890 IHC (p) WB Rabbit Ig AA 875-904, C-Term Log in to see Polyclonal 1
0.13341247 ABIN967994 IF IP IHC WB Mouse IgG1 AA 279-472 Log in to see 35-EphA4 3
0.13341247 ABIN1724712 ELISA WB Mouse IgG1 AA 777-986 Log in to see 7D3D4 3
0.13341247 ABIN2788864 IHC WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2854457 WB Rabbit IgG Internal Region Log in to see Polyclonal
0.13341247 ABIN1107105 WB Mouse IgG2b Log in to see 6C1B6 1
0.13341247 ABIN1107104 WB Mouse IgG1 Log in to see 7D3D4 3
0.13341247 ABIN931478 ELISA WB Mouse IgG1 AA 777-986 Log in to see 7D3D4
0.13341247 ABIN2160952 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.13341247 ABIN2160950 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.13341247 ABIN2160951 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.13341247 ABIN560755 ELISA WB Mouse IgG1 kappa AA 887-986, partial Log in to see 6H7
0.13341247 ABIN3182395 ELISA WB Rabbit IgG pTyr596 Log in to see Polyclonal
0.13341247 ABIN1843498 ELISA WB Mouse IgG1 AA 777-986 Log in to see
0.13341247 ABIN1847866 ELISA WB Rabbit Internal Region, pTyr596 Log in to see Polyclonal
0.0011728023 ABIN2869317 WB Mouse IgG1 AA 777-986 Log in to see 2
0.0011728023 ABIN1496031 WB Rabbit IgG Internal Region Log in to see Polyclonal

Top referenced anti-EPH Receptor A4 antibody for Western Blotting

Similar anti-EPH Receptor A4 Antibodies

Application / Reactivity Human
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 12 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 89 Antibodies

General

Antigen EPH Receptor A4 (EPHA4) Antibodies
Epitope AA 40-70, N-Term
(30), (21), (20), (14), (13), (11), (8), (8), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(141), (46), (31), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Host Rabbit
(139), (19), (3), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This EPH Receptor A4 antibody is un-conjugated
(7), (7), (7), (6), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(109), (76), (67), (31), (15), (11), (11), (6), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-EPH Receptor A4 Antibody

Target Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 Antibody (ABIN391889) Images
Predicted Reactivity Chicken,Mouse (Murine),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This EphA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 AA from the N-terminal region of human EphA4.
Clone RB1587
Isotype Ig

Target Details EPH Receptor A4

Product Details anti-EPH Receptor A4 Antibody Application Details Handling References for anti-EPH Receptor A4 Antibody (ABIN391889) Images back to top
Antigen
Alternative Name EphA4 (EPHA4 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Synonyms: Ephrin type-A receptor 4 , Tyrosine-protein kinase receptor SEK, Receptor protein-tyrosine kinase HEK8
Molecular Weight 109859 Da
Gene ID 2043
UniProt P54764
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Handling References for anti-EPH Receptor A4 Antibody (ABIN391889) Images back to top
Application Notes Suggested dilutions:
WB = 1:1000, IF = 1:100, FACS = 1:10-50, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details References for anti-EPH Receptor A4 Antibody (ABIN391889) Images back to top
Format Liquid
Concentration 1.11 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-EPH Receptor A4 Antibody (ABIN391889)

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling Images back to top
Background publications

Prevost, Woulfe, Tanaka, Brass: "Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9219-24, 2002

Images

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 Antibody (ABIN391889) back to top
Supplier Images
Western Blotting (WB) image for anti-EPH Receptor A4 antibody (EPHA4) (AA 40-70) (ABIN391889) Western blot analysis of anti-EphA4 N-term Pab (ABIN391889) in HeLa cell lysate. EphA...
Western Blotting (WB) image for anti-EPH Receptor A4 antibody (EPHA4) (AA 40-70) (ABIN391889) Western blot analysis of EphA4 (arrow) using EphA4 Antibody (N-term) (ABIN391889). 29...
Immunofluorescence (IF) image for anti-EPH Receptor A4 antibody (EPHA4) (AA 40-70) (ABIN391889) Methanol/Acetone fixed human stem cell is used in IF to detect Eph4A (blue) and endot...