anti-Human EPH Receptor B4 antibody for Western Blotting

Recommended EPH Receptor B4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B4 (EPHB4) Antibodies
  • htk
  • myk1
  • ephB4a
  • tyro11
  • ephb4b
  • MGC130997
  • AI042935
  • Htk
  • MDK2
  • Myk1
  • Tyro11
  • HTK
  • MYK1
  • TYRO11
  • EPH receptor B4
  • EPH receptor B4 L homeolog
  • EPH receptor B4 S homeolog
  • Eph receptor B4
  • EPHB4
  • ephb4.L
  • ephb4.S
  • ephb4
  • Ephb4
Epitope
AA 16-539
20
18
16
14
13
7
6
5
4
3
3
3
3
2
2
1
1
1
Reactivity
Human
149
67
10
1
1
1
1
Host
Goat
70
67
38
17
2
Clonality
Polyclonal
Conjugate
This EPH Receptor B4 antibody is un-conjugated
7
7
6
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
137
106
34
25
22
19
5
4
4
3
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN4899281
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0849469 ABIN359817 EIA IHC (p) WB Rabbit Ig Log in to see Polyclonal 5
3.0849469 ABIN391924 IHC (p) WB Rabbit Ig Log in to see Polyclonal 5
3.0849469 ABIN387814 WB Mouse IgG1 kappa Log in to see 67CT23-5-8 5
3.0849469 ABIN1533783 ELISA WB Rabbit IgG AA 571-620 Log in to see Polyclonal
3.0849469 ABIN969108 ICC ELISA WB Mouse IgG1 Log in to see 5B5 1
3.0849469 ABIN1724713 ELISA WB Mouse IgG1 AA 562-612 Log in to see 7H4A6 3
3.0849469 ABIN969107 IHC ELISA WB Mouse IgG2a Log in to see 5B8F7 2
3.0849469 ABIN1107113 EIA IHC (p) WB Mouse IgG2a Log in to see 5B8F7 2
3.0849469 ABIN356403 EIA WB Mouse IgG1 Log in to see 67CT23-5-8
3.0849469 ABIN3184512 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0849469 ABIN1107115 IF WB Mouse IgG1 Log in to see 5B5 2
3.0849469 ABIN614445 EIA IF IHC (p) WB Mouse IgG1 Extracellular Domain Log in to see 5B5
3.0849469 ABIN1107114 WB Mouse IgG1 Log in to see 7H4A6 3
3.0849469 ABIN2706114 WB Rabbit Center Log in to see Polyclonal
3.0849469 ABIN498286 IF IHC (p) WB Rabbit Log in to see Polyclonal
3.0849469 ABIN4308720 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.0849469 ABIN1872556 FACS IHC WB Rabbit IgG Log in to see Polyclonal
3.0849469 ABIN4308719 WB Rabbit IgG N-Term Log in to see Polyclonal
3.0849469 ABIN931479 ELISA WB Mouse IgG1 AA 562-612 Log in to see 7H4A6
3.0849469 ABIN4270058 ELISA WB Mouse IgG1 Log in to see 7H4A6

General

Antigen EPH Receptor B4 (EPHB4) Antibodies
Epitope AA 16-539
(20), (18), (16), (14), (13), (7), (6), (5), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Reactivity Human
(149), (67), (10), (1), (1), (1), (1)
Host Goat
(70), (67), (38), (17), (2)
Clonality
Polyclonal
Conjugate This EPH Receptor B4 antibody is un-conjugated
(7), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(137), (106), (34), (25), (22), (19), (5), (4), (4), (3), (2), (2), (1), (1)
Pubmed 6 references available
Supplier Log in to see

Product Details anti-EPH Receptor B4 Antibody

Target Details EPH Receptor B4 Application Details Handling References for anti-EPH Receptor B4 antibody (ABIN4899281) Images
Specificity Detects human EphB4 in direct ELISAs and Western blots. In direct ELISAs, less than 10 % cross-reactivity with recombinant mouse (rm) EphB4 is observed and less than 1 % cross-reactivity with recombinant human (rh) EphA4, rhEphA3, rhEphB2, and rhEphB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human EphB4, Leu16-Ala539, Accession # P54760
Isotype IgG

Target Details EPH Receptor B4

Product Details anti-EPH Receptor B4 Antibody Application Details Handling References for anti-EPH Receptor B4 antibody (ABIN4899281) Images back to top
Antigen
Alternative Name EphB4 (EPHB4 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, ephrin type-B receptor 4, hepatoma transmembrane kinase, Htk, HTKephrin receptor EphB4, Mdk2, Myk1, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3, Tyro11, Tyrosine-protein kinase receptor HTK, Tyrosine-protein kinase TYRO11
Gene ID 2050
UniProt P54760
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Handling References for anti-EPH Receptor B4 antibody (ABIN4899281) Images back to top
Application Notes Western Blot 2 μg/mL
Simple Western 50 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details References for anti-EPH Receptor B4 antibody (ABIN4899281) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor B4 antibody (ABIN4899281)

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling Images back to top
Product cited in:

Fuggetta, Bordignon, Cottarelli, Macchi, Frezza, Cordiali-Fei, Ensoli, Ciafrè, Marino-Merlo, Mastino, Ravagnan: "Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol." in: Journal of experimental & clinical cancer research : CR, Vol. 35, Issue 1, pp. 118, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Martin-Almedina, Martinez-Corral, Holdhus, Vicente, Fotiou, Lin, Petersen, Simpson, Hoischen, Gilissen, Jeffery, Atton, Karapouliou, Brice, Gordon, Wiseman, Wedin, Rockson, Jeffery, Mortimer, Snyder et al.: "EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis. ..." in: The Journal of clinical investigation, Vol. 126, Issue 8, pp. 3080-8, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Boyer-Di Ponio, El-Ayoubi, Glacial, Ganeshamoorthy, Driancourt, Godet, Perrière, Guillevic, Couraud, Uzan: "Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes." in: PLoS ONE, Vol. 9, Issue 1, pp. e84179, 2014 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

He, Kumar, Zhou, Krasnoperov, Ryan, Gill, Hinton: "Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro." in: Investigative ophthalmology & visual science, Vol. 51, Issue 1, pp. 543-52, 2010 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Suzuki, Abe, Imagama, Nomura, Tanizaki, Minami, Hayakawa, Ito, Katsumi, Yamamoto, Emi, Kiyoi, Naoe: "BCR-ABL-independent and RAS / MAPK pathway-dependent form of imatinib resistance in Ph-positive acute lymphoblastic leukemia cell line with activation of EphB4." in: European journal of haematology, Vol. 84, Issue 3, pp. 229-38, 2010 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kwan Tat, Pelletier, Amiable, Boileau, Lajeunesse, Duval, Martel-Pelletier: "Activation of the receptor EphB4 by its specific ligand ephrin B2 in human osteoarthritic subchondral bone osteoblasts." in: Arthritis and rheumatism, Vol. 58, Issue 12, pp. 3820-30, 2008 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Images

Product Details anti-EPH Receptor B4 Antibody Target Details EPH Receptor B4 Application Details Handling References for anti-EPH Receptor B4 antibody (ABIN4899281) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor B4 (EPHB4) (AA 16-539) antibody (ABIN4899281) Detection of EphB4 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast can...
Simple Western (SimWes) image for anti-EPH Receptor B4 (EPHB4) (AA 16-539) antibody (ABIN4899281) Detection of Human EphB4 by Simple WesternTM. Simple Western lane view shows lysates ...
Western Blotting (WB) image for anti-EPH Receptor B4 (EPHB4) (AA 16-539) antibody (ABIN4899281) Detection of Human EphB4 by Western Blot. Western blot shows lysates of K562 human ch...
Immunohistochemistry (IHC) image for anti-EPH Receptor B4 (EPHB4) (AA 16-539) antibody (ABIN4899281) EphB4 in Human Kidney. EphB4 was detected in immersion fixed paraffin-embedded sectio...