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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||19 Antibodies||12 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||2 Antibodies|
|Immunofluorescence (IF)||12 Antibodies||13 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||10 Antibodies||11 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Western Blotting (WB)||32 Antibodies|
|Antigen||Ephrin A5 (EFNA5) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Ephrin A5 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Ephrin A5 AntibodyTarget Details Ephrin A5 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Ephrin-A5 protein. (region surrounding Pro63)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Target Details Ephrin A5Product Details anti-Ephrin A5 Antibody Application Details Handling Images back to top
|Alternative Name||Ephrin-A5 (EFNA5 Antibody Abstract)|
|Background||Ephrin A5, a member of the ephrin family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.Synonyms: AL-1, EFNA5, EPH-related receptor tyrosine kinase ligand 7, EPL7, EPLG7, LERK-7, LERK7|
|Molecular Weight||approx. 25 kDa|
Application DetailsProduct Details anti-Ephrin A5 Antibody Target Details Ephrin A5 Handling Images back to top
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - /200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Ephrin A5 Antibody Target Details Ephrin A5 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Ephrin A5 Antibody Target Details Ephrin A5 Application Details Handling back to top