|Application / Reactivity||Rat (Rattus)||Mouse (Murine)||Human|
|Western Blotting (WB)||22 Antibodies||28 Antibodies||59 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies||4 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||9 Antibodies||4 Antibodies||3 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||2 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies|
|Antigen||Ephrin B1 (EFNB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Ephrin B1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Ephrin B1 AntibodyTarget Details Ephrin B1 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of Ephrin-B protein. (region surrounding Gln324)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: Ephrin-B2
|Purification||Affinity Chromatography using epitope-specific immunogen.|
Target Details Ephrin B1Product Details anti-Ephrin B1 Antibody Application Details Handling Images back to top
|Alternative Name||Ephrin-B1 (EFNB1 Antibody Abstract)|
|Background||Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development.Synonyms: CEK5 LIgand, CEK5-L, EFL-3, EFL3, EFNB1, ELK-L, EPH-related receptor tyrosine kinase ligand 2, EPLG2, LERK-2, LERK2|
|Molecular Weight||approx. 59 kDa|
Application DetailsProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Handling Images back to top
ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-Ephrin B1 Antibody Target Details Ephrin B1 Application Details Handling back to top