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anti-Cow (Bovine) Ephrin B2 antibody for Western Blotting

Recommended Ephrin B2 Antibody (supplied by: Log in to see )

Antigen
Ephrin B2 (EFNB2) Antibodies
  • EFNB2
  • efnb2
  • efnb2a
  • efnb2b
  • ELF-2
  • ephrin-B2
  • ephrinB2
  • Epl5
  • Eplg5
  • EPLG5
  • eplg5
  • Htk-L
  • htk-l
  • HTKL
  • htkl
  • LERK-5
  • LERK5
  • Lerk5
  • lerk5
  • MGC68890
  • NLERK-1
Epitope
Internal Region
15
12
11
10
10
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
102
42
27
2
2
1
1
1
1
1
Host
Goat
92
14
2
Clonality
Polyclonal
Conjugate
This Ephrin B2 antibody is un-conjugated
3
3
3
3
3
3
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
86
39
34
18
8
8
2
1
Supplier
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Catalog No. ABIN499146
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011731827 ABIN2562370 ELISA WB Goat IgG Internal Region Log in to see Polyclonal

Top referenced anti-Ephrin B2 antibody for Western Blotting

Similar anti-Ephrin B2 Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus) Cow (Bovine) Dog (Canine) Pig (Porcine)
Western Blotting (WB) 36 Antibodies 85 Antibodies 23 Antibodies 2 Antibodies 3 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 33 Antibodies 10 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 18 Antibodies 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 8 Antibodies

General

Antigen Ephrin B2 (EFNB2) Antibodies
Epitope Internal Region
(15), (12), (11), (10), (10), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(102), (42), (27), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(92), (14), (2)
Clonality
Polyclonal
Conjugate This Ephrin B2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(86), (39), (34), (18), (8), (8), (2), (1)
Pubmed 1 reference available
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Product Details anti-Ephrin B2 Antibody

Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 Antibody (ABIN499146) Images
Sequence CPKVDSKTVG QYE
Specificity Recognizes EFNB2
Cross-Reactivity (Details) Species reactivity (expected):Canine, Bovine, Pig.
Species reactivity (tested):Human, Mouse, Rat.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide with sequence from the internal region of the protein sequence according to NP_004084.1. Genename: EFNB2

Target Details Ephrin B2

Product Details anti-Ephrin B2 Antibody Application Details Handling References for anti-Ephrin B2 Antibody (ABIN499146) Images back to top
Antigen
Alternative Name Ephrin-B2 (EFNB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5
Molecular Weight 36.9kDa (NP_004084.1)
Gene ID 1948
NCBI Accession NP_004084
UniProt P52799
Pathways RTK Signaling

Application Details

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Handling References for anti-Ephrin B2 Antibody (ABIN499146) Images back to top
Application Notes Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 35 kDa band observed inHuman, Mouse and Rat Lung lysates.
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details References for anti-Ephrin B2 Antibody (ABIN499146) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Ephrin B2 Antibody (ABIN499146)

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling Images back to top
Background publications

Nakada, Anderson, Demuth, Nakada, Reavie, Drake, Hoelzinger, Berens: "The phosphorylation of ephrin-B2 ligand promotes glioma cell migration and invasion." in: International journal of cancer, Vol. 126, Issue 5, pp. 1155-65, 2010

Images

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 Antibody (ABIN499146) back to top
Supplier Images
Western Blotting (WB) image for anti-Ephrin B2 antibody (EFNB2) (Internal Region) (ABIN499146) AP21298PU-N EFNB2 antibody staining of Rat Lung lysate at 0.1µg/ml (35µg protein in R...