anti-Human Ephrin B2 antibody for Western Blotting

Recommended Ephrin B2 Antibody (supplied by: Log in to see )

Antigen
Ephrin B2 (EFNB2) Antibodies
  • EFNB2
  • efnb2
  • ephrin-B2
  • htkl
  • eplg5
  • htk-l
  • lerk5
  • ephrinB2
  • efnb2a
  • MGC68890
  • efnb2b
  • ELF-2
  • Epl5
  • Eplg5
  • Htk-L
  • LERK-5
  • Lerk5
  • NLERK-1
  • EPLG5
  • HTKL
  • LERK5
  • ephrin-B2
  • ephrin-B2 precursor
  • ephrin B2
  • EFNB2
  • efnb2
  • efnb2-a
  • LOC100357479
  • Efnb2
Epitope
pTyr330
15
11
10
10
9
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
104
49
31
5
5
5
3
3
3
2
2
1
1
Host
Rabbit
90
18
2
Clonality
Polyclonal
Conjugate
This Ephrin B2 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
91
39
35
26
10
8
3
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN616035
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9709127 ABIN655599 IF IHC (p) WB Rabbit Ig AA 157-186, Center Log in to see Polyclonal 1
2.9709127 ABIN616033 WB Rabbit pTyr316 Log in to see Polyclonal 3
2.9709127 ABIN4900008 CyTOF FACS ICC WB Goat IgG AA 27-227 Log in to see Polyclonal 13
2.9709127 ABIN4308729 ELISA WB Goat Log in to see Polyclonal 1
2.9709127 ABIN4308730 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
2.9709127 ABIN2854523 WB Rabbit IgG Center Log in to see Polyclonal
2.9709127 ABIN2464896 ELISA WB Goat Internal Region Log in to see Polyclonal
2.9709127 ABIN3197628 WB Rabbit Log in to see Polyclonal
2.9709127 ABIN1870105 WB Rabbit IgG pTyr316 Log in to see Polyclonal
2.9709127 ABIN1870107 WB Rabbit IgG pTyr330 Log in to see Polyclonal
2.9709127 ABIN1876870 IHC WB Rabbit IgG Log in to see Polyclonal
2.9709127 ABIN1867699 ICC IHC IP WB Rabbit IgG AA 42-333 Log in to see Polyclonal
2.9709127 ABIN4308731 ICC IF IHC (p) WB Rabbit Log in to see Polyclonal
2.9709127 ABIN2703587 IHC ELISA WB Mouse IgG1 kappa Extracellular Domain Log in to see PA349-18-4-3
2.9709127 ABIN560701 ELISA WB Mouse AA 28-127, partial Log in to see Polyclonal
2.9709127 ABIN2562370 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
2.9709127 ABIN2406450 IHC WB Rabbit IgG Log in to see Polyclonal
2.9709127 ABIN3020178 WB Rabbit pTyr316 Log in to see Polyclonal
2.9709127 ABIN3020181 WB Rabbit pTyr330 Log in to see Polyclonal
2.9709127 ABIN274039 WB Rabbit IgG Log in to see Polyclonal

General

Antigen Ephrin B2 (EFNB2) Antibodies
Epitope pTyr330
(15), (11), (10), (10), (9), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(104), (49), (31), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(90), (18), (2)
Clonality
Polyclonal
Conjugate This Ephrin B2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(91), (39), (35), (26), (10), (8), (3), (2), (2), (2), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-Ephrin B2 Antibody

Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN616035) Images
Sequence N-I-Y(p)-Y-K
Specificity This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse
Purification Affinity-chromatography using epitope-specific peptide
Immunogen Peptide sequence around phosphorylation site of tyrosine 330 derived from Human Ephrin- B2

Target Details Ephrin B2

Product Details anti-Ephrin B2 Antibody Application Details Handling References for anti-Ephrin B2 antibody (ABIN616035) Images back to top
Antigen
Alternative Name Ephrin-B2 (EFNB2 Antibody Abstract)
Background Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms, however, not all variants have been fully described.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5
Molecular Weight 37 kDa
Gene ID 1948
NCBI Accession NP_004084
UniProt P52799
Pathways RTK Signaling, Regulation of Muscle Cell Differentiation

Application Details

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Handling References for anti-Ephrin B2 antibody (ABIN616035) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details References for anti-Ephrin B2 antibody (ABIN616035) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References for anti-Ephrin B2 antibody (ABIN616035)

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling Images back to top
Background publications

Chrencik, Brooun, Kraus, Recht, Kolatkar, Han, Seifert, Widmer, Auer, Kuhn: "Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 28185-92, 2006

Kertesz, Krasnoperov, Reddy, Leshanski, Kumar, Zozulya, Gill: "The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth." in: Blood, Vol. 107, Issue 6, pp. 2330-8, 2006

Noren, Lu, Freeman, Koolpe, Pasquale: "Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5583-8, 2004

Images

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN616035) back to top
Supplier Images
Western Blotting (WB) image for anti-Ephrin B2 (EFNB2) (pTyr330) antibody (ABIN616035) Western blot analysis of extracts from HT29 cells, untreated or treated with UV using...