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anti-Mouse (Murine) Ephrin B2 antibody for Immunofluorescence

Recommended Ephrin B2 Antibody (supplied by: Log in to see )

Antigen
Ephrin B2 (EFNB2) Antibodies
  • EFNB2
  • efnb2
  • efnb2a
  • efnb2b
  • ELF-2
  • ephrin-B2
  • ephrinB2
  • Epl5
  • Eplg5
  • EPLG5
  • eplg5
  • Htk-L
  • htk-l
  • HTKL
  • htkl
  • LERK-5
  • LERK5
  • Lerk5
  • lerk5
  • MGC68890
  • NLERK-1
Epitope
AA 328-332
15
12
12
10
10
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
99
40
25
3
3
2
1
1
1
Host
Rabbit
88
14
2
Clonality
Polyclonal
Conjugate
This Ephrin B2 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunofluorescence (IF), Western Blotting (WB)
82
38
33
16
7
6
3
1
Supplier
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Catalog No. ABIN197418
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010857971 ABIN362834 IF WB Rabbit IgG AA 328-332 Log in to see Polyclonal 4
0.0010857971 ABIN274040 IF WB Rabbit IgG Log in to see Polyclonal
0.0010857971 ABIN274041 IF WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Ephrin B2 antibody for Immunofluorescence

Similar anti-Ephrin B2 Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Western Blotting (WB) 34 Antibodies 82 Antibodies 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 32 Antibodies 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (IF) 17 Antibodies 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Ephrin B2 (EFNB2) Antibodies
Epitope AA 328-332
(15), (12), (12), (10), (10), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(99), (40), (25), (3), (3), (2), (1), (1), (1)
Host Rabbit
(88), (14), (2)
Clonality
Polyclonal
Conjugate This Ephrin B2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), Western Blotting (WB)
(82), (38), (33), (16), (7), (6), (3), (1)
Pubmed 3 references available
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Product Details anti-Ephrin B2 Antibody

Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 Antibody (ABIN197418) Images
Specificity This antibody AP02708PU detects endogenous levels of total Ephrin-B2 protein.
Purification Immunoaffinity Chromatography using epitope-specific immunogen.
Immunogen Peptide Sequence around amino acids 328~332 (N-I-Y-Y-K) derived from Human Ephrin-B2.

Target Details Ephrin B2

Product Details anti-Ephrin B2 Antibody Application Details Handling References for anti-Ephrin B2 Antibody (ABIN197418) Images back to top
Antigen
Alternative Name Ephrin-B2 (EFNB2 Antibody Abstract)
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5
Gene ID 1948
NCBI Accession NP_004084
UniProt P52799
Pathways RTK Signaling

Application Details

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Handling References for anti-Ephrin B2 Antibody (ABIN197418) Images back to top
Application Notes Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details References for anti-Ephrin B2 Antibody (ABIN197418) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-Ephrin B2 Antibody (ABIN197418)

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling Images back to top
Background publications

Chrencik, Brooun, Kraus, Recht, Kolatkar, Han, Seifert, Widmer, Auer, Kuhn: "Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 28185-92, 2006

Kertesz, Krasnoperov, Reddy, Leshanski, Kumar, Zozulya, Gill: "The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth." in: Blood, Vol. 107, Issue 6, pp. 2330-8, 2006

Noren, Lu, Freeman, Koolpe, Pasquale: "Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5583-8, 2004

Images

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Supplier Images
 image for anti-Ephrin B2 antibody (EFNB2) (AA 328-332) (ABIN197418) anti-Ephrin B2 (EFNB2) (AA 328-332) antibody
 image for anti-Ephrin B2 antibody (EFNB2) (AA 328-332) (ABIN197418) anti-Ephrin B2 (EFNB2) (AA 328-332) antibody (Image 2)