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|Antigen||Ephrin B2 (EFNB2) Antibodies|
|Epitope||AA 328-332 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Ephrin B2 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-Ephrin B2 AntibodyTarget Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN197418) Images
|Specificity||This antibody AP02708PU detects endogenous levels of total Ephrin-B2 protein.|
|Purification||Immunoaffinity Chromatography using epitope-specific immunogen.|
|Immunogen||Peptide Sequence around amino acids 328~332 (N-I-Y-Y-K) derived from Human Ephrin-B2.|
Target Details Ephrin B2Product Details anti-Ephrin B2 Antibody Application Details Handling References for anti-Ephrin B2 antibody (ABIN197418) Images back to top
|Alternative Name||Ephrin-B2 (EFNB2 Antibody Abstract)|
|Background||Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5|
|Pathways||RTK Signaling, Regulation of Muscle Cell Differentiation|
Application DetailsProduct Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Handling References for anti-Ephrin B2 antibody (ABIN197418) Images back to top
Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details References for anti-Ephrin B2 antibody (ABIN197418) Images back to top
|Buffer||PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at -20 °C.|
References for anti-Ephrin B2 antibody (ABIN197418)Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling Images back to top
Chrencik, Brooun, Kraus, Recht, Kolatkar, Han, Seifert, Widmer, Auer, Kuhn: "Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity." in: The Journal of biological chemistry, Vol. 281, Issue 38, pp. 28185-92, 2006
Kertesz, Krasnoperov, Reddy, Leshanski, Kumar, Zozulya, Gill: "The soluble extracellular domain of EphB4 (sEphB4) antagonizes EphB4-EphrinB2 interaction, modulates angiogenesis, and inhibits tumor growth." in: Blood, Vol. 107, Issue 6, pp. 2330-8, 2006
Noren, Lu, Freeman, Koolpe, Pasquale: "Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 15, pp. 5583-8, 2004
ImagesProduct Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN197418) back to top