anti-Human ERBB4 antibody for Enzyme Immunoassay

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • erbb4
  • si:ch211-227n20.1
  • Her4
  • c-erbB-4
  • HER4
  • p180erbB4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4a (avian)
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
  • receptor tyrosine-protein kinase ErbB-4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 4
  • ERBB4
  • erbb4a
  • erbb4
  • Tsp_13582
  • Erbb4
Epitope
pTyr1188
55
37
36
33
21
21
19
17
15
15
13
11
7
6
6
6
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
392
179
145
12
9
6
6
6
5
3
3
3
2
2
2
1
1
Host
Rabbit
301
96
1
Clonality
Polyclonal
Conjugate
This ERBB4 antibody is un-conjugated
21
18
17
15
11
10
9
9
7
7
7
5
5
5
5
5
5
4
4
3
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
211
144
126
91
68
59
28
23
14
5
4
2
2
1
1
Supplier
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Catalog No. ABIN358140
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359846 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
1 ABIN5552127 EIA IHC (p) WB Rabbit Ig Fraction pTyr1162 Log in to see Polyclonal

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Epitope pTyr1188
(55), (37), (36), (33), (21), (21), (19), (17), (15), (15), (13), (11), (7), (6), (6), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(392), (179), (145), (12), (9), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(301), (96), (1)
Clonality
Polyclonal
Conjugate This ERBB4 antibody is un-conjugated
(21), (18), (17), (15), (11), (10), (9), (9), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(211), (144), (126), (91), (68), (59), (28), (23), (14), (5), (4), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling ProductDetails: References for anti-ERBB4 antibody (ABIN358140) Images
Specificity This antibody detects HER4 pTyr1188.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1188 of human HER4.
Isotype Ig

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling ProductDetails: References for anti-ERBB4 antibody (ABIN358140) Images back to top
Antigen
Alternative Name ERBB4 / HER4 (ERBB4 Antibody Abstract)
Background The HER4/ERBB4 gene is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2, and ERBB3. It encodes a receptor for NDF/heregulin.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4
Molecular Weight 146808 Da
Gene ID 2066, 9606
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling ProductDetails: References for anti-ERBB4 antibody (ABIN358140) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details ProductDetails: References for anti-ERBB4 antibody (ABIN358140) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-ERBB4 antibody (ABIN358140)

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Background publications

Tanimura, Nakago, Murakoshi, Takekida, Moriyama, Matsuo, Hashimoto, Maruo: "Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy." in: European journal of endocrinology / European Federation of Endocrine Societies, Vol. 151, Issue 1, pp. 93-101, 2004

Hughes, Thomas, Giordano, Baker, McDonagh: "Cell surface expression of epidermal growth factor receptor and Her-2 with nuclear expression of Her-4 in primary osteosarcoma." in: Cancer research, Vol. 64, Issue 6, pp. 2047-53, 2004

Cheng, Tikhomirov, Zhou, Carpenter: "Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38421-7, 2003

Chaudhury, Gerecke, Wyss, Morgan, Gordon, Carroll: "Neuregulin-1 and erbB4 immunoreactivity is associated with neuritic plaques in Alzheimer disease brain and in a transgenic model of Alzheimer disease." in: Journal of neuropathology and experimental neurology, Vol. 62, Issue 1, pp. 42-54, 2003

Komuro, Nagai, Navin, Sudol: "WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33334-41, 2003

Images

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling ProductDetails: References for anti-ERBB4 antibody (ABIN358140) back to top
Supplier Images
 image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1188) antibody (ABIN358140) anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1188) antibody
 image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1188) antibody (ABIN358140) anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1188) antibody (Image 2)