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anti-Human ERBB4 antibody for Enzyme Immunoassay

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • c-erbB-4
  • erbb4
  • HER4
  • Her4
  • p180erbB4
  • si:ch211-227n20.1
Epitope
pTyr1162
61
30
28
27
21
18
17
13
13
13
7
7
6
5
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
301
116
108
3
3
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
268
36
Clonality
Polyclonal
Conjugate
This ERBB4 antibody is un-conjugated
19
16
15
12
11
10
5
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
155
105
103
58
56
25
21
19
12
2
2
2
1
1
1
1
Supplier
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Catalog No. ABIN358139
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088294124 ABIN358140 EIA IHC (p) WB Rabbit Ig pTyr1188 Log in to see Polyclonal 5
0.00088294124 ABIN359846 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5

Top referenced anti-ERBB4 antibody for Enzyme Immunoassay

  • Human Polyclonal ERBB4 Primary Antibody for EIA, IHC (p) - ABIN358139 (1 Publications): Burns, Li, Cheney, Ou, Franlin-Pfeifer, Kuklin, Zhang: Choice of fixative is crucial to successful immunohistochemical detection of phosphoproteins in paraffin-embedded tumor tissues. in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2009 (PubMed)

  • Human Polyclonal ERBB4 Primary Antibody for EIA, IHC (p) - ABIN358140 (5 Publications): Tanimura, Nakago, Murakoshi, Takekida, Moriyama, Matsuo, Hashimoto, Maruo: Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy. in European journal of endocrinology / European Federation of Endocrine Societies 2004 (PubMed)

  • Human Polyclonal ERBB4 Primary Antibody for EIA, IHC (p) - ABIN359846 (5 Publications): Williams, Trout, Gallo, Pitfield, Bryant, Penington, Riese: A constitutively active ErbB4 mutant inhibits drug-resistant colony formation by the DU-145 and PC-3 human prostate tumor cell lines. in Cancer letters 2003 (PubMed)

Similar anti-ERBB4 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 102 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Functional Studies (Func) 2 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 58 Antibodies
Immunohistochemistry (IHC) 107 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 61 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Neutralization (Neut) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 160 Antibodies

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Epitope pTyr1162
(61), (30), (28), (27), (21), (18), (17), (13), (13), (13), (7), (7), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(301), (116), (108), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(268), (36)
Clonality
Polyclonal
Conjugate This ERBB4 antibody is un-conjugated
(19), (16), (15), (12), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(155), (105), (103), (58), (56), (25), (21), (19), (12), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling References for anti-ERBB4 Antibody (ABIN358139) Images
Specificity This antibody detects HER4 pTyr1162.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1162 of human HER4.
Isotype Ig

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling References for anti-ERBB4 Antibody (ABIN358139) Images back to top
Antigen
Alternative Name ERBB4 / HER4 (ERBB4 Antibody Abstract)
Background HER4/ERBB4 is a member of the type I receptor tyrosine kinase subfamily that includes EGFR, ERBB2 and ERBB3. It is a receptor for NDF/heregulin.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4
Molecular Weight 146808 Da
Gene ID 2066, 9606
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling References for anti-ERBB4 Antibody (ABIN358139) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details References for anti-ERBB4 Antibody (ABIN358139) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ERBB4 Antibody (ABIN358139)

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Background publications

Burns, Li, Cheney, Ou, Franlin-Pfeifer, Kuklin, Zhang: "Choice of fixative is crucial to successful immunohistochemical detection of phosphoproteins in paraffin-embedded tumor tissues." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 57, Issue 3, pp. 257-64, 2009

Images

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling References for anti-ERBB4 Antibody (ABIN358139) back to top
Supplier Images
 image for anti-ERBB4 antibody (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian)) (pTyr1162) (ABIN358139) anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1162) antibody
 image for anti-ERBB4 antibody (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian)) (pTyr1162) (ABIN358139) anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1162) antibody (Image 2)