anti-Human ERBB4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • erbb4
  • si:ch211-227n20.1
  • Her4
  • c-erbB-4
  • HER4
  • p180erbB4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4a (avian)
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
  • receptor tyrosine-protein kinase ErbB-4
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 4
  • ERBB4
  • erbb4a
  • erbb4
  • Tsp_13582
  • Erbb4
Epitope
C-Term
54
37
37
33
24
21
21
19
17
15
15
11
7
6
6
6
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
403
179
145
12
9
6
6
6
5
3
3
3
2
2
2
1
1
Host
Rabbit
301
106
2
Clonality
Polyclonal
Conjugate
This ERBB4 antibody is un-conjugated
21
18
17
16
12
10
10
10
8
8
8
5
5
5
5
5
5
5
5
3
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
212
144
126
91
70
68
28
23
15
6
5
2
2
1
1
Supplier
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Catalog No. ABIN359846
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.749182 ABIN213400 IHC (p) Rabbit Internal Region Log in to see Polyclonal
12.749182 ABIN1714916 IF (p) IHC (p) Rabbit IgG AA 460-510 Log in to see Polyclonal
12.749182 ABIN498233 IF IHC (p) Rabbit Log in to see Polyclonal
12.749182 ABIN4309317 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
12.749182 ABIN446812 ICC IF IHC (p) IHC Rabbit Log in to see Polyclonal
12.749182 ABIN4309316 IHC (p) Rabbit Log in to see Polyclonal
12.749182 ABIN213439 IHC (p) Rabbit C-Term Log in to see Polyclonal
12.749182 ABIN213401 IHC (p) Rabbit Internal Region Log in to see Polyclonal
12.749182 ABIN4309314 IHC (p) Rabbit C-Term Log in to see Polyclonal
12.749182 ABIN4309326 CyTOF ELISA FACS ICC IHC IHC (p) WB Mouse IgG1 Log in to see 5G6B4
12.749182 ABIN4309315 IHC (p) Rabbit Log in to see Polyclonal
1 ABIN358140 EIA IHC (p) WB Rabbit Ig pTyr1188 Log in to see Polyclonal 5
1 ABIN389572 IF IHC (p) WB Rabbit Ig pTyr1162 Log in to see Polyclonal 2
1 ABIN391950 IHC (p) WB Rabbit Ig AA 25-55, N-Term Log in to see Polyclonal 4
1 ABIN737436 FACS IF (p) IHC (p) Rabbit IgG AA 1210-1260 Log in to see Polyclonal 1
1 ABIN391951 IHC (p) WB Rabbit Ig AA 1276-1308, C-Term Log in to see Polyclonal 3
1 ABIN443062 ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 1
1 ABIN1048578 IHC (p) Rabbit Internal Region Log in to see Polyclonal
1 ABIN5552127 EIA IHC (p) WB Rabbit Ig Fraction pTyr1162 Log in to see Polyclonal
1 ABIN2888849 IF IHC (p) ELISA Rabbit IgG AA 1250-1299 Log in to see Polyclonal

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Epitope C-Term
(54), (37), (37), (33), (24), (21), (21), (19), (17), (15), (15), (11), (7), (6), (6), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(403), (179), (145), (12), (9), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(301), (106), (2)
Clonality
Polyclonal
Conjugate This ERBB4 antibody is un-conjugated
(21), (18), (17), (16), (12), (10), (10), (10), (8), (8), (8), (5), (5), (5), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(212), (144), (126), (91), (70), (68), (28), (23), (15), (6), (5), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling References for anti-ERBB4 antibody (ABIN359846) Images
Specificity This antibody reacts to ErbB4.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ErbB4.
Isotype Ig

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling References for anti-ERBB4 antibody (ABIN359846) Images back to top
Antigen
Alternative Name ERBB4 / HER4 (ERBB4 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4
Gene ID 2066, 9606
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling References for anti-ERBB4 antibody (ABIN359846) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details References for anti-ERBB4 antibody (ABIN359846) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-ERBB4 antibody (ABIN359846)

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Background publications

Williams, Trout, Gallo, Pitfield, Bryant, Penington, Riese: "A constitutively active ErbB4 mutant inhibits drug-resistant colony formation by the DU-145 and PC-3 human prostate tumor cell lines." in: Cancer letters, Vol. 192, Issue 1, pp. 67-74, 2003

Thomas, Chouinard, Cox, Parsons, Stallings-Mann, Garcia, Jove, Wharen: "Spontaneous activation and signaling by overexpressed epidermal growth factor receptors in glioblastoma cells." in: International journal of cancer. Journal international du cancer, Vol. 104, Issue 1, pp. 19-27, 2003

Ni, Yuan, Carpenter: "Role of the ErbB-4 carboxyl terminus in gamma-secretase cleavage." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4561-5, 2003

Cheng, Tikhomirov, Zhou, Carpenter: "Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38421-7, 2003

Komuro, Nagai, Navin, Sudol: "WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33334-41, 2003

Images

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling References for anti-ERBB4 antibody (ABIN359846) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (C-Term) antibody (ABIN359846) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (C-Term) antibody (ABIN359846) Western blot analysis of anti-ErbB4 Pab in T-47D cell lysate. ErbB4 (arrow) was detec...