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anti-Human ERBB4 antibody for Immunoprecipitation

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • c-erbB-4
  • erbb4
  • HER4
  • Her4
  • p180erbB4
  • si:ch211-227n20.1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
301
115
107
3
3
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
269
35
Clonality
Monoclonal
Conjugate
This ERBB4 antibody is un-conjugated
19
16
15
12
11
10
5
5
5
5
5
5
5
5
5
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
156
105
103
58
57
25
20
18
12
3
2
1
1
1
1
1
Supplier
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Catalog No. ABIN180326
$ 554.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN180325 Func FACS IP Mouse IgG1 Log in to see Ab77 (H4-77-16) 2
0.00089235755 ABIN493051 IP WB Rabbit Log in to see Polyclonal 3
0.00089235755 ABIN189393 ICC FACS IF IP WB Rabbit IgG C-Term, Cytoplasmic Domain Log in to see E200
0.00089235755 ABIN251106 IHC (p) IP IHC WB Mouse IgG2b AA 1250-1264 Log in to see HFR1
0.00089235755 ABIN211921 IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN445074 IHC (p) IP IHC WB Rabbit Tyr1162 Log in to see EP2270Y
0.00089235755 ABIN2261694 IP IHC WB Mouse IgG2b AA 1250-1264 Log in to see 9H232
0.00089235755 ABIN385438 FACS IP Biotin Mouse IgG1 Log in to see H4-77-16
0.00089235755 ABIN385435 FACS IP Mouse IgG1 Log in to see H4-77-16
0.00089235755 ABIN385439 FACS IP Biotin Mouse IgG1 Log in to see H4-77-16
0.00089235755 ABIN1893715 IP Rabbit IgG AA 1100-1150 Log in to see Polyclonal
0.00089235755 ABIN1893723 IP PLA WB Rabbit IgG C-Term Log in to see Polyclonal
0.00089235755 ABIN385436 FACS IP Mouse IgG1 Log in to see H4-77-16
0.00089235755 ABIN385441 FACS IP Mouse IgG1 Extracellular Domain Log in to see H4-77-16
0.00089235755 ABIN2261682 FACS IP Mouse IgG1 Log in to see 3F166
0.00089235755 ABIN2261684 FACS IP Mouse IgG2a Log in to see 3F167
0.00089235755 ABIN2261683 FACS IP Mouse IgG1 Log in to see 3F166
0.00089235755 ABIN2261695 IP WB Rabbit IgG AA 1285-1308 Log in to see Polyclonal
0.00089235755 ABIN2749948 IP ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.00089235755 ABIN2261681 FACS IP Neut Mouse IgG2a Log in to see 0-T-196

Top referenced anti-ERBB4 antibody for Immunoprecipitation

Similar anti-ERBB4 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 18 Antibodies 19 Antibodies
Flow Cytometry (FACS) 4 Antibodies 4 Antibodies
Functional Studies (Func) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 10 Antibodies
Immunofluorescence (IF) 19 Antibodies 19 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 58 Antibodies 58 Antibodies
Immunohistochemistry (IHC) 23 Antibodies 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 38 Antibodies

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(301), (115), (107), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(269), (35)
Clonality (Clone)
Monoclonal   ( )
Conjugate This ERBB4 antibody is un-conjugated
(19), (16), (15), (12), (11), (10), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(156), (105), (103), (58), (57), (25), (20), (18), (12), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling References for anti-ERBB4 Antibody (ABIN180326) Images
Specificity The antibody does not interfere with the receptor-heregulin binding and is particularly well suited for Immunoprecipitation of unoccupied as well as the occupied c-erbB-4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity, and efficiently stimulates internalization of c-erbB-4.
Cellular Localization: Cytoplasmic and cell membrane.
Characteristics Synonyms: p180erbB4, c-ErbB-4, HER-4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-likeprotein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4
Purification Protein G Chromatography.
Immunogen Extracellular fragment of recombinant Human c-erbB-4/HER-4 oncoprotein. Remarks:
Clone Ab77 (H4-77-16)
Isotype IgG1

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling References for anti-ERBB4 Antibody (ABIN180326) Images back to top
Antigen
Alternative Name ERBB4 / HER4 (ERBB4 Antibody Abstract)
Background Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu. ErbB4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4
Gene ID 2066
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling References for anti-ERBB4 Antibody (ABIN180326) Images back to top
Application Notes Accelerates Internalization of c-erbB-4 . Flow Cytometry (Ref.2). Immunoprecipitation Use Ab at 2 μg/mg protein lysate (Use Protein G)Stimulates Tyrosine Kinase Activity . Recommended Positive Control: T47D or MDA-MB-453 Cells. Not suitable for Western Blotting. For Functional Studies use Azide Free antibody Cat. # ABIN180325!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details References for anti-ERBB4 Antibody (ABIN180326) Images back to top
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-ERBB4 Antibody (ABIN180326)

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Background publications

Tang, Concepcion, Milan, Gong, Montgomery, Lippman: "Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo." in: Cancer research, Vol. 59, Issue 20, pp. 5315-22, 1999

Chen, Levkowitz, Tzahar, Karunagaran, Lavi, Ben-Baruch, Leitner, Ratzkin, Bacus, Yarden: "An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7620-9, 1996