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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||18 Antibodies||19 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies||5 Antibodies|
|Functional Studies (Func)||2 Antibodies||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies||9 Antibodies|
|Immunofluorescence (IF)||21 Antibodies||21 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||63 Antibodies||63 Antibodies|
|Immunohistochemistry (IHC)||25 Antibodies||27 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Western Blotting (WB)||40 Antibodies|
|Antigen||V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ERBB4 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
|2 references available|
|Supplier||Log in to see|
Product Details anti-ERBB4 AntibodyTarget Details ERBB4 Application Details Handling References for anti-ERBB4 Antibody (ABIN180326) Images
The antibody does not interfere with the receptor-heregulin binding and is particularly well suited for Immunoprecipitation of unoccupied as well as the occupied c-erbB-4 receptor. It reacts with intact cells, stimulates tyrosine kinase activity, and efficiently stimulates internalization of c-erbB-4.
Cellular Localization: Cytoplasmic and cell membrane.
|Characteristics||Synonyms: p180erbB4, c-ErbB-4, HER-4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-likeprotein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4|
|Purification||Protein G Chromatography.|
|Immunogen||Extracellular fragment of recombinant Human c-erbB-4/HER-4 oncoprotein. Remarks:|
Target Details ERBB4Product Details anti-ERBB4 Antibody Application Details Handling References for anti-ERBB4 Antibody (ABIN180326) Images back to top
|Alternative Name||ERBB4 / HER4 (ERBB4 Antibody Abstract)|
|Background||Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu. ErbB4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-ERBB4 Antibody Target Details ERBB4 Handling References for anti-ERBB4 Antibody (ABIN180326) Images back to top
Accelerates Internalization of c-erbB-4 . Flow Cytometry (Ref.2). Immunoprecipitation Use Ab at 2 μg/mg protein lysate (Use Protein G)Stimulates Tyrosine Kinase Activity . Recommended Positive Control: T47D or MDA-MB-453 Cells. Not suitable for Western Blotting. For Functional Studies use Azide Free antibody Cat. # ABIN180325!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERBB4 Antibody Target Details ERBB4 Application Details References for anti-ERBB4 Antibody (ABIN180326) Images back to top
|Buffer||10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-ERBB4 Antibody (ABIN180326)Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Tang, Concepcion, Milan, Gong, Montgomery, Lippman: "Ribozyme-mediated down-regulation of ErbB-4 in estrogen receptor-positive breast cancer cells inhibits proliferation both in vitro and in vivo." in: Cancer research, Vol. 59, Issue 20, pp. 5315-22, 1999
Chen, Levkowitz, Tzahar, Karunagaran, Lavi, Ben-Baruch, Leitner, Ratzkin, Bacus, Yarden: "An immunological approach reveals biological differences between the two NDF/heregulin receptors, ErbB-3 and ErbB-4." in: The Journal of biological chemistry, Vol. 271, Issue 13, pp. 7620-9, 1996