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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||5 Antibodies||4 Antibodies||4 Antibodies|
|Cytometry by Time of Flight (CyTOF)||2 Antibodies||1 Antibodies|
|Western Blotting (WB)||202 Antibodies||75 Antibodies||48 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Immunoprecipitation (IP)||22 Antibodies||15 Antibodies||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||85 Antibodies||48 Antibodies||27 Antibodies|
|Immunohistochemistry (IHC)||139 Antibodies||43 Antibodies||27 Antibodies|
|Antigen||V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This ERBB4 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Product Details anti-ERBB4 AntibodyTarget Details ERBB4 Application Details Handling Images
|Specificity||This antibody detects endogenous levels of ErbB-4 protein.(region surrounding Glu1280)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % (by SDS-PAGE)|
Target Details ERBB4Product Details anti-ERBB4 Antibody Application Details Handling Images back to top
|Alternative Name||ERBB4 / HER4 (ERBB4 Antibody Abstract)|
|Background||The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4|
|Molecular Weight||approx. 150 kDa|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-ERBB4 Antibody Target Details ERBB4 Handling Images back to top
Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.|
ImagesProduct Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling back to top