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anti-Cow (Bovine) FGF2 antibody for Western Blotting

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • bFGF
  • BFGF
  • Fgf-2
  • FGF-2
  • Fgfb
  • FGFB
  • HBGF-2
Epitope
Middle Region
15
14
13
12
10
8
8
6
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
312
56
43
37
22
17
4
2
1
1
1
Host
Rabbit
186
152
8
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
34
18
13
8
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
252
214
97
59
35
27
20
20
18
17
12
12
11
7
7
3
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2777106
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.124960825 ABIN1582147 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 2
0.0012207722 ABIN269699 IHC (p) IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.0012207722 ABIN187644 IHC (fro) RIA WB Mouse IgG1 Log in to see BFM-2
0.0012207722 ABIN191893 IHC (fro) IHC (p) RIA WB Rabbit Log in to see Polyclonal
0.0012207722 ABIN269333 BP ELISA Neut RIA WB Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN609600 IHC (fro) IHC (p) WB Mouse IgG1, kappa Log in to see
0.0012207722 ABIN329819 ELISA WB Rabbit IgG AA 1-24 Log in to see Polyclonal
0.0012207722 ABIN601319 IHC RIA WB Rabbit Log in to see Polyclonal
0.0012207722 ABIN200929 IHC (fro) IHC (p) WB Mouse IgG1, kappa Log in to see
0.0012207722 ABIN4271985 ELISA WB Alexa Fluor 405 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271992 ELISA WB FITC Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271994 ELISA WB DyLight 488 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271995 ELISA WB DyLight 755 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271988 ELISA WB Alexa Fluor 700 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271991 ELISA WB DyLight 650 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271996 ELISA WB PerCP Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271999 ELISA WB DyLight 350 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4272001 ELISA WB DyLight 405LS Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271986 ELISA WB Alexa Fluor 488 Mouse IgG1 Log in to see FB-8
0.0012207722 ABIN4271987 ELISA WB Alexa Fluor 647 Mouse IgG1 Log in to see FB-8

Top referenced anti-FGF2 antibody for Western Blotting

Similar anti-FGF2 Antibodies

Application / Reactivity Cow (Bovine) Human Mouse (Murine) Rat (Rattus) Chicken Dog (Canine) Rabbit Zebrafish (Danio rerio)
Western Blotting (WB) 32 Antibodies 225 Antibodies 33 Antibodies 25 Antibodies 5 Antibodies 3 Antibodies 9 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 7 Antibodies 3 Antibodies 2 Antibodies 2 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Neutralization (Neut) 22 Antibodies 6 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 20 Antibodies 2 Antibodies 2 Antibodies

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Epitope Middle Region
(15), (14), (13), (12), (10), (8), (8), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(312), (56), (43), (37), (22), (17), (4), (2), (1), (1), (1)
Host Rabbit
(186), (152), (8), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(34), (18), (13), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(252), (214), (97), (59), (35), (27), (20), (20), (18), (17), (12), (12), (11), (7), (7), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images
Blocking Peptide Blocking peptide for this product available: ABIN976833
Sequence RLESNNYNTY RSRKYTSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Bovine : 90 %, Horse : 90 %, Pig : 90 %, Sheep : 90 %, African clawed frog : 80 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against FGF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FGF2

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Antigen
Alternative Name FGF2 (FGF2 Antibody Abstract)
Background The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Molecular Weight 31 kDa
Gene ID 2247
NCBI Accession NM_002006, NP_001997
UniProt P09038
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 288 AA

Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FGF2 Antibody (ABIN2777106)

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Background publications

Goodger, Robinson, Murphy, Gasiunas, Harmer, Blundell, Pye, Gallagher: "Evidence that heparin saccharides promote FGF2 mitogenesis through two distinct mechanisms." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13001-8, 2008

Images

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) back to top
Supplier Images
Western Blotting (WB) image for anti-FGF2 antibody (Fibroblast Growth Factor 2 (Basic)) (Middle Region) (ABIN2777106) WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...