anti-Dog (Canine) FGF2 antibody for Western Blotting

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • Fgf-2
  • Fgfb
  • bFGF
  • FGF-2
  • BFGF
  • FGFB
  • HBGF-2
  • fibroblast growth factor 2
  • fibroblast growth factor 2 (basic)
  • Fgf2
  • fgf2
  • FGF2
Epitope
Middle Region
16
16
12
10
10
8
7
6
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
326
72
59
37
24
19
4
2
2
1
1
1
1
1
Host
Rabbit
195
154
8
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
33
19
13
9
6
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
263
218
100
64
33
27
26
19
15
14
13
13
11
7
7
4
4
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2777106
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.906104 ABIN967725 IF IP IHC WB Mouse IgG2a AA 1-155 Log in to see 6-basic FGF 5
2.906104 ABIN967726 IF IP IHC WB Mouse IgG2a AA 1-155 Log in to see 6-basic FGF 5

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Epitope Middle Region
(16), (16), (12), (10), (10), (8), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish
(326), (72), (59), (37), (24), (19), (4), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(195), (154), (8), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(33), (19), (13), (9), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(263), (218), (100), (64), (33), (27), (26), (19), (15), (14), (13), (13), (11), (7), (7), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling ProductDetails: References for anti-FGF2 antibody (ABIN2777106) Images
Blocking Peptide Blocking peptide for this product available: ABIN976833
Sequence RLESNNYNTY RSRKYTSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 93%, Zebrafish: 86%
Characteristics This is a rabbit polyclonal antibody against FGF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FGF2

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling ProductDetails: References for anti-FGF2 antibody (ABIN2777106) Images back to top
Antigen
Alternative Name FGF2 (FGF2 Antibody Abstract)
Background The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BFGF, FGFB, HBGF-2
Protein Interaction Partner: TUBG1, HMT1, PRMT1, CRYAB, THBS1, UBC, API5, CEP57, FGFRL1, FGFBP1, CXCL13, RPL6, RPS19, PTX3, NRP1, GPC4, RPS6KA3, GPC3, VTN, SDC3, SDC1, PF4, HSPG2, SDC2, FGFR1, FGFR4, FGFR3, FGF2, CSNK2A2, CSNK2B, CSNK2A1, CD44, SDC4,
Protein Size: 288
Molecular Weight 31 kDa
Gene ID 2247
NCBI Accession NM_002006, NP_001997
UniProt P09038
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling ProductDetails: References for anti-FGF2 antibody (ABIN2777106) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 288 AA

Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details ProductDetails: References for anti-FGF2 antibody (ABIN2777106) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-FGF2 antibody (ABIN2777106)

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Background publications

Goodger, Robinson, Murphy, Gasiunas, Harmer, Blundell, Pye, Gallagher: "Evidence that heparin saccharides promote FGF2 mitogenesis through two distinct mechanisms." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13001-8, 2008

Images

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling ProductDetails: References for anti-FGF2 antibody (ABIN2777106) back to top
Supplier Images
Western Blotting (WB) image for anti-Fibroblast Growth Factor 2 (Basic) (FGF2) (Middle Region) antibody (ABIN2777106) WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...