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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||3 Antibodies|
|Blocking Peptide (BP)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||17 Antibodies|
|Flow Cytometry (FACS)||20 Antibodies|
|Functional Studies (Func)||7 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||7 Antibodies|
|Immunodiffusion (ID)||2 Antibodies|
|Immunofluorescence (IF)||24 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||13 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies|
|Immunohistochemistry (IHC)||83 Antibodies|
|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies|
|Epitope||AA 143-288 Alternatives|
|Conjugate||This FGF2 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
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Product Details anti-FGF2 AntibodyTarget Details FGF2 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Human.
Gene Name: fibroblast growth factor 2(basic)
Protein Name: Fibroblast growth factor 2
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human FGF2 recombinant protein(Position: P143-S288).|
Target Details FGF2Product Details anti-FGF2 Antibody Application Details Handling Images back to top
|Alternative Name||FGF2 (FGF2 Antibody Abstract)|
|Background||FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96 % and 97 % amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor(FGF) family. Fibroblast growth factors(FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus|
Application DetailsProduct Details anti-FGF2 Antibody Target Details FGF2 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Human
Neut: Species: Human
IP: Species: Human
Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling back to top