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anti-Rabbit FGF2 antibody for Western Blotting

Recommended FGF2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
  • Fgf-2
  • Fgfb
  • bFGF
  • FGF-2
  • BFGF
  • FGFB
  • HBGF-2
  • fibroblast growth factor 2
  • fibroblast growth factor 2 (basic)
  • Fgf2
  • fgf2
  • FGF2
Epitope
Middle Region
16
13
12
10
10
8
8
6
5
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
323
71
58
37
24
19
4
2
2
1
1
1
1
1
Host
Rabbit
192
152
8
6
1
1
Clonality
Polyclonal
Conjugate
This FGF2 antibody is un-conjugated
34
18
13
8
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
268
217
99
64
33
28
24
22
20
17
13
13
11
7
7
4
3
2
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN2777106
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1003003 ABIN1582147 IHC ELISA WB Rabbit Middle Region Log in to see Polyclonal 2
3.1003003 ABIN1858868 ICC IHC (fro) IHC (p) ELISA WB Guinea Pig IgG AA 26-153 Log in to see Polyclonal
1 ABIN726425 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 20-60 Log in to see Polyclonal 12
1 ABIN468753 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN726434 IHC (p) WB HRP Rabbit IgG AA 20-60 Log in to see Polyclonal
1 ABIN726427 IHC (p) WB Biotin Rabbit IgG AA 20-60 Log in to see Polyclonal
1 ABIN2620825 ELISA WB Biotin Guinea Pig IgG AA 26-153 Log in to see Polyclonal
1 ABIN2601939 ELISA WB FITC Guinea Pig IgG AA 26-153 Log in to see Polyclonal
1 ABIN2601935 ELISA WB Guinea Pig IgG AA 26-153 Log in to see Polyclonal
1 ABIN2266503 IC IHC ELISA WB Guinea Pig IgG AA 26-153 Log in to see Polyclonal
1 ABIN2929255 ELISA IF/ICC IHC WB Guinea Pig AA 26-153 Log in to see Polyclonal

Top referenced anti-FGF2 antibody for Western Blotting

Similar anti-FGF2 Antibodies

Application / Reactivity Human Rabbit Mouse (Murine) Cow (Bovine) Rat (Rattus) Chicken Dog (Canine) Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 31 Antibodies 240 Antibodies 46 Antibodies 35 Antibodies 5 Antibodies 3 Antibodies 12 Antibodies 1 Antibodies
Radioimmunoassay (RIA) 7 Antibodies 4 Antibodies 2 Antibodies 2 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Neutralization (Neut) 25 Antibodies 6 Antibodies
Immunostaining (ISt) 1 Antibodies

General

Antigen Fibroblast Growth Factor 2 (Basic) (FGF2) Antibodies
Epitope Middle Region
(16), (13), (12), (10), (10), (8), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(323), (71), (58), (37), (24), (19), (4), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(192), (152), (8), (6), (1), (1)
Clonality
Polyclonal
Conjugate This FGF2 antibody is un-conjugated
(34), (18), (13), (8), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(268), (217), (99), (64), (33), (28), (24), (22), (20), (17), (13), (13), (11), (7), (7), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-FGF2 Antibody

Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images
Blocking Peptide Blocking peptide for this product available: ABIN976833
Sequence RLESNNYNTY RSRKYTSWYV ALKRTGQYKL GSKTGPGQKA ILFLPMSAKS
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Bovine : 90 %, Horse : 90 %, Pig : 90 %, Sheep : 90 %, African clawed frog : 80 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against FGF2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FGF2

Target Details FGF2

Product Details anti-FGF2 Antibody Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Antigen
Alternative Name FGF2 (FGF2 Antibody Abstract)
Background The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
Molecular Weight 31 kDa
Gene ID 2247
NCBI Accession NM_002006, NP_001997
UniProt P09038
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-FGF2 Antibody Target Details FGF2 Handling References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 288 AA

Restrictions For Research Use only

Handling

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details References for anti-FGF2 Antibody (ABIN2777106) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FGF2 Antibody (ABIN2777106)

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling Images back to top
Background publications

Goodger, Robinson, Murphy, Gasiunas, Harmer, Blundell, Pye, Gallagher: "Evidence that heparin saccharides promote FGF2 mitogenesis through two distinct mechanisms." in: The Journal of biological chemistry, Vol. 283, Issue 19, pp. 13001-8, 2008

Images

Product Details anti-FGF2 Antibody Target Details FGF2 Application Details Handling References for anti-FGF2 Antibody (ABIN2777106) back to top
Supplier Images
Western Blotting (WB) image for anti-FGF2 antibody (Fibroblast Growth Factor 2 (Basic)) (Middle Region) (ABIN2777106) WB Suggested Anti-FGF2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...