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|Application / Reactivity||Cow (Bovine)||Dog (Canine)||Goat||Guinea Pig||Human||Monkey||Mouse (Murine)||Pig (Porcine)||Rabbit||Rat (Rattus)||Sheep (Ovine)||Wild boar (Sus scrofa)|
|ELISA||8 ELISA Kits||1 ELISA Kits||1 ELISA Kits||1 ELISA Kits||30 ELISA Kits||1 ELISA Kits||15 ELISA Kits||7 ELISA Kits||7 ELISA Kits||14 ELISA Kits||1 ELISA Kits||2 ELISA Kits|
|Antigen||Fibroblast Growth Factor 2 (Basic) (FGF2) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||15.62-1000 pg/mL|
|Minimum Detection Limit||15.62 pg/mL|
|9 references available|
|Supplier||Log in to see|
Product Details FGF2 ELISA KitTarget details Application Details Handling ProductDetails: References for FGF2 Kit (ABIN414089) Images
|Purpose||The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of FGF2 in Serum,Plasma,Biological Fluids|
|Sample Type||Serum, Plasma, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 2, Basic (FGF2).
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Fibroblast Growth Factor 2, Basic (FGF2) and analogues was observed.|
|Material not included||
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|Alternative Name||FGF2 (FGF2 ELISA Kit Abstract)|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus|
Application DetailsProduct Details FGF2 ELISA Kit Target details Handling ProductDetails: References for FGF2 Kit (ABIN414089) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic (FGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 2, Basic (FGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 2, Basic (FGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve on log-log graph paper, with FGF2 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, such as curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is indeed the independent variable while O.D. value is the dependent variable. Further, in this part, in order to help the customer perform the assay more visual, we provide the customer with the raw data (not the log of data). However, plotting log of the data to construct the curve will be recommended. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). This curve is provided for demonstration only. The customers should establish their own standard curve for each test conducted.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibroblast Growth Factor 2, Basic (FGF2) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
HandlingProduct Details FGF2 ELISA Kit Target details Application Details ProductDetails: References for FGF2 Kit (ABIN414089) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
|Expiry Date||6 months|
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|Product cited in:||
Beck, Hotowy, Sawosz, Grodzik, Wierzbicki, Kutwin, Jaworski, Chwalibog: "Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos." in: Archives of animal nutrition, Vol. 69, Issue 1, pp. 57-68, 2015
Wierzbicki, Sawosz, Grodzik, Hotowy, Prasek, Jaworski, Sawosz, Chwalibog: "Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis." in: International journal of nanomedicine, Vol. 8, pp. 3427-35, 2013
Sawosz, Pineda, Hotowy, Jaworski, Prasek, Sawosz, Chwalibog: "Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis." in: Archives of animal nutrition, Vol. 67, Issue 5, pp. 347-55, 2013
Grodzik, Sawosz, Sawosz, Hotowy, Wierzbicki, Kutwin, Jaworski, Chwalibog: "Nano-nutrition of chicken embryos. The effect of in ovo administration of diamond nanoparticles and L-glutamine on molecular responses in chicken embryo pectoral muscles." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 23033-44, 2013
Zielinska, Sawosz, Grodzik, Wierzbicki, Gromadka, Hotowy, Sawosz, Lozicki, Chwalibog: "Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis." in: International journal of nanomedicine, Vol. 6, pp. 3163-72, 2012
Zielinska, Sawosz, Grodzik, Balcerak, Wierzbicki, Skomial, Sawosz, Chwalibog: "Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis." in: Archives of animal nutrition, Vol. 66, Issue 1, pp. 1-13, 2012
Lean, Murgatroyd, Rothnie, Reid, Harvey: "Metabolic and thyroidal responses to mild cold are abnormal in obese diabetic women." in: Clinical endocrinology, Vol. 28, Issue 6, pp. 665-73, 1989
Howell: "Spotting sinister spots. A challenge to dermatologists to examine every new patient at increased risk for signs of early melanoma." in: Journal of the American Academy of Dermatology, Vol. 15, Issue 4 Pt 1, pp. 722-6, 1986
Jeffreys, Wilson, Thein: "Hypervariable 'minisatellite' regions in human DNA." in: Nature, Vol. 314, Issue 6006, pp. 67-73, 1985
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