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Human FGF7 ELISA Kit

Recommended FGF7 Product (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor 7 (FGF7) ELISA Kits
  • FGF-7
  • HBGF-7
  • Kgf
  • kgf
  • KGF
Epitope
AA 32-194
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
19
11
10
9
4
3
3
3
3
2
2
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1672808
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.30628014 ABIN414511 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.63-1000 pg/mL 15.63 pg/mL Log in to see
0.30628014 ABIN1114918 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.30628014 ABIN1380037 Sandwich ELISA Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.30628014 ABIN2708209 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN366845 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see 1
0.0012155735 ABIN511339 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 pg/mL 100 pg/mL Log in to see 1
0.0012155735 ABIN3032921 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.55-400 pg/mL 0.55 pg/mL Log in to see
0.0012155735 ABIN624978 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Urine Log in to see 1
0.0012155735 ABIN455653 Serum, Tissue Homogenate, Biological Fluids 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN1569829 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.625-1000 pg/mL 15.625 pg/mL Log in to see
0.0012155735 ABIN2703018 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 6-1.500 pg/mL 6 pg/mL Log in to see
0.0012155735 ABIN2042464 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012155735 ABIN2114396 Cell Lysate, Serum, Plasma 32-2000 pg/mL 32 pg/mL Log in to see
0.0012155735 ABIN2684262 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Log in to see
0.0012155735 ABIN2533424 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 15.62-1000 pg/mL 15.62 pg/mL Log in to see
0.0012155735 ABIN2535810 Tissue Homogenate, Cell Lysate, Biological Fluids 5-400 pg/mL 5 pg/mL Log in to see
0.0012155735 ABIN2089493 Sandwich ELISA Log in to see
0.0012155735 ABIN2082998 Sandwich ELISA Log in to see
0.0012155735 ABIN2947504 31.25-2000 pg/mL 31.25 pg/mL Log in to see

Top referenced FGF7 ELISA Kit for ELISA

  • Human FGF7 ELISA Kit for Sandwich ELISA - ABIN511339 (1 Publications): Stachon, Wang, Eppig, Langenbucher, Bischoff, Seitz, Szentmáry: KGF, FGFb, VEGF, HGF and TGFβ1 secretion of human keratocytes following photodynamic inactivation (PDI) in vitro. in Graefe's archive for clinical and experimental ophthalmology 2013 (PubMed)

  • Human FGF7 ELISA Kit for Sandwich ELISA - ABIN624978 (1 Publications): Nazarov, Lee, Soupene, Etemad, Knapik, Green, Bashkirova, Fang, Matthay, Kuypers, Serikov: Multipotent stromal stem cells from human placenta demonstrate high therapeutic potential. in Stem cells translational medicine 2012 (PubMed)

  • Human FGF7 ELISA Kit for Sandwich ELISA - ABIN366845 (1 Publications): López, Alonso, García de Galdeano, Smith-Zubiaga: Melanocytes from dark and light skin respond differently after ultraviolet B irradiation: effect of keratinocyte-conditioned medium. in Photodermatology, photoimmunology & photomedicine 2015 (PubMed)

  • Human FGF7 ELISA Kit for Sandwich ELISA - ABIN1672808 (1 Publications): Mattei, deLapeyrière, Bresnick, Dickson, Birnbaum, Mason: Mouse Fgf7 (fibroblast growth factor 7) and Fgf8 (fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively. in Mammalian genome : official journal of the International Mammalian Genome Society 1995 (PubMed)

Similar FGF7 ELISA Kits

Application / Reactivity Human
ELISA 20 ELISA Kits

General

Antigen Fibroblast Growth Factor 7 (FGF7) ELISA Kits
Epitope AA 32-194
Reactivity Human
Kits with alternative reactivity to:
(19), (11), (10), (9), (4), (3), (3), (3), (3), (2), (2), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details FGF7 ELISA Kit

Target details Application Details Handling References for FGF7 Kit (ABIN1672808) Images
Purpose For quantitative detection of human FGF7 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant human FGF7
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <4pg/mL
Components
  • 96-well plate precoated with anti- human FGF7 antibod - 1
  • Lyophilized recombinant human FGF7 standar - 10ng/tubex2
  • Biotinylated anti- human FGF7 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: E.coli
Immunogen sequence: C32-T194

Target details

Product Details FGF7 ELISA Kit Application Details Handling References for FGF7 Kit (ABIN1672808) Images back to top
Antigen
Alternative Name FGF7 (FGF7 ELISA Kit Abstract)
Background Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Gene ID 2252
UniProt P21781
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details FGF7 ELISA Kit Target details Handling References for FGF7 Kit (ABIN1672808) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human FGF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FGF7 has been precoated onto 96-well plates. Standards(E.coli, C32-T194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FGF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FGF7 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human FGF7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FGF7 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 6.40, CV(%): 4.1
  • Sample 2: n=16, Mean(pg/ml): 645, Standard deviation: 27.09, CV(%): 4.2
  • Sample 3: n=16, Mean(pg/ml): 1278, Standard deviation: 70.29, CV(%): 5.6,
  • Sample 1: n=24, Mean(pg/ml): 147, Standard deviation: 12.35, CV(%): 8.4
  • Sample 2: n=24, Mean(pg/ml): 649, Standard deviation: 39.59, CV(%): 6.1
  • Sample 3: n=24, Mean(pg/ml): 1226, Standard deviation: 78.46, CV(%): 6.4
Restrictions For Research Use only

Handling

Product Details FGF7 ELISA Kit Target details Application Details References for FGF7 Kit (ABIN1672808) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FGF7 Kit (ABIN1672808)

Product Details FGF7 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Mattei, deLapeyrière, Bresnick, Dickson, Birnbaum, Mason: "Mouse Fgf7 (fibroblast growth factor 7) and Fgf8 (fibroblast growth factor 8) genes map to chromosomes 2 and 19 respectively." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 6, Issue 3, pp. 196-7, 1995

Images

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Supplier Images
ELISA image for FGF7 ELISA Kit (Fibroblast Growth Factor 7) (ABIN1672808) Human FGF7/KGF PicoKine ELISA Kit standard curve