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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||9 Antibodies||45 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||5 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies||53 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||25 Antibodies|
|Western Blotting (WB)||17 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Antigen||Fibroblast Growth Factor Receptor 3 (FGFR3) Antibodies|
|Epitope||AA 110-129, N-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This FGFR3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FGFR3 AntibodyTarget Details FGFR3 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Fibroblast growth factor receptor 3(FGFR3) detection. Tested with WB, IHC-P, ICC in Human,Rat.
Gene Name: fibroblast growth factor receptor 3
Protein Name: Fibroblast growth factor receptor 3
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human FGFR3(110-129aa RQRLTQRVLCHFSVRVTDAP), different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.|
Target Details FGFR3Product Details anti-FGFR3 Antibody Application Details Handling Images back to top
|Alternative Name||FGFR3 (FGFR3 Antibody Abstract)|
|Background||Fibroblast growth factor receptor 3, also known as CD333, is a protein that in humans is encoded by the FGFR3 gene. This gene encodes a member of the fibroblast growth factor receptor(FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. The FGFR3 gene is mapped to the HD region on chromosome 4p16.3. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.|
|Research Area||Stem Cells, Growth Factors, Angiogenesis|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FGFR3 Antibody Target Details FGFR3 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGFR3 Antibody Target Details FGFR3 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-FGFR3 Antibody Target Details FGFR3 Application Details Handling back to top