anti-Human FLT1 antibody for Immunofluorescence

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT1
  • VEGFR1
  • AI323757
  • Flt-1
  • VEGFR-1
  • sFlt1
  • FLT
  • FLT-1
  • flt1b
  • fms-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • FMS-like tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • vascular endothelial growth factor receptor-1
  • FLT1
  • CpipJ_CPIJ012087
  • Flt1
  • flt1
  • FLT-1
Epitope
Internal Region, AA 800-900
51
39
17
15
15
14
14
11
10
9
6
5
5
5
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
415
148
87
16
13
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
240
214
36
5
4
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
29
14
14
13
13
12
11
9
9
9
9
8
8
8
6
5
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
317
224
104
75
71
63
56
40
15
13
13
6
5
4
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN151960
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.326143 ABIN449639 ICC IF IP ELISA WB Rabbit Log in to see Polyclonal
12.326143 ABIN515758 IF WB Mouse AA 1-687, full length Log in to see Polyclonal
12.326143 ABIN5544843 IF ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN155162 EIA IF IP WB Biotin Mouse IgG1 Extracellular Domain Log in to see FLTEWF
1 ABIN115152 EIA IF IP WB Mouse IgG1 Extracellular Domain Log in to see FLTEWF 3
1 ABIN1109475 IHC (fro) IF WB Mouse IgG1 Log in to see V12 2
1 ABIN115153 EIA IF IP WB Mouse IgG1 Extracellular Domain Log in to see FLTEWI (EWI) 3
1 ABIN269284 ELISA ICC IF WB Mouse IgG1 Log in to see Flt-1-EWC 1
1 ABIN115151 EIA FACS IF Mouse IgG1 AA 1-5, N-Term, Extracellular Domain Log in to see FLTEIC
1 ABIN360964 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN360966 IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN269286 ICC IF IP ELISA WB Mouse IgG1 Log in to see EWF
1 ABIN152342 CyTOF ELISA FACS ICC IF Mouse IgG1 Log in to see EIC
1 ABIN355002 IF IP ELISA WB Mouse IgG1 Log in to see EWF
1 ABIN4365227 ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 800-900, Internal Region Log in to see Polyclonal
1 ABIN4365229 FACS IF Mouse IgG1 kappa Log in to see FLT1-658
1 ABIN4365228 ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 800-900, Internal Region Log in to see Polyclonal
1 ABIN4365232 FACS IF Mouse IgG1 kappa Log in to see FLT1-659
1 ABIN4365205 ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 800-900, Internal Region Log in to see Polyclonal
1 ABIN4365208 ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 800-900, Internal Region Log in to see Polyclonal

Top referenced anti-FLT1 antibody for Immunofluorescence

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Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 3 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 180 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 50 Antibodies
Functional Studies (Func) 6 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 35 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 70 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope Internal Region, AA 800-900
(51), (39), (17), (15), (15), (14), (14), (11), (10), (9), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(415), (148), (87), (16), (13), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(240), (214), (36), (5), (4), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(29), (14), (14), (13), (13), (12), (11), (9), (9), (9), (9), (8), (8), (8), (6), (5), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(317), (224), (104), (75), (71), (63), (56), (40), (15), (13), (13), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling ProductDetails: References for anti-FLT1 antibody (ABIN151960) Images
Specificity This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling ProductDetails: References for anti-FLT1 antibody (ABIN151960) Images back to top
Antigen
Alternative Name VEGFR1/Flt-1 (FLT1 Antibody Abstract)
Background Gene Symbol: FLT1
Gene ID 2321
UniProt P35969
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling ProductDetails: References for anti-FLT1 antibody (ABIN151960) Images back to top
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-ParaffinThis VEGF Receptor 1 antibody is useful for Western blot, where a band is seen at ~180-230 kDa (the theoretical molecular weight of mouse VEGFR1 is ~150 kDa and human VEGFR1 is ~151 kDa). The difference in MWs is likely due to glycosylation or other post translational modifications. With CSF-1R/VEGFR2 chimera transfected lysates a doublet is seen at ~150 kDa representing VEGFR-2. An unknown band is also seen at ~50 kDa. Optimal working dilutions should be determined by the investigator.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR1 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details ProductDetails: References for anti-FLT1 antibody (ABIN151960) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-FLT1 antibody (ABIN151960)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008

Ptaszynska, Pendrak, Bandle, Stracke, Roberts: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (Sample species: Human). Further details: Western Blotting

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000

Background publications

Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling ProductDetails: References for anti-FLT1 antibody (ABIN151960) back to top
Supplier Images
Western Blotting (WB) image for anti-FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (Internal Region) (ABIN151960) Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunofluorescence (IF) image for anti-FLT1 antibody (Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor)) (Internal Region) (ABIN151960) Immunocytochemistry/Immunofluorescence: VEGF R1/Flt-1 Antibody [ABIN151960] - VEGF R1...