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anti-Human FLT1 antibody for Immunoprecipitation

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • AI323757
  • FLT
  • FLT-1
  • Flt-1
  • FLT1
  • flt1b
  • sFlt1
  • VEGFR-1
  • VEGFR1
Epitope
Extracellular Domain
32
20
13
10
9
9
8
5
5
5
5
5
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
245
74
64
12
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Mouse
189
85
7
4
1
1
Clonality
Monoclonal
Conjugate
This FLT1 antibody is un-conjugated
15
10
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
171
132
55
47
37
25
20
16
11
11
11
5
2
2
1
1
1
1
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Catalog No. ABIN115152
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008946119 ABIN155162 EIA IF IP WB Biotin Mouse IgG1 Extracellular Domain Log in to see FLTEWF
0.0008946119 ABIN115153 EIA IF IP WB Mouse IgG1 Extracellular Domain Log in to see FLTEWI (EWI) 3
0.0008946119 ABIN115150 EIA IP WB Mouse IgG1 Log in to see FLTEWC
0.0008946119 ABIN258537 ICC FACS IHC (fro) IF IHC (p) IP IHC WB Rabbit IgG N-Term Log in to see Y103
0.0008946119 ABIN317122 EIA IHC (fro) IP Mouse IgG1 Extracellular Domain Log in to see Flt-19 1
0.0008946119 ABIN360860 EIA IP WB Biotin Mouse IgG1 Log in to see FLTEWC
0.0008946119 ABIN115683 EIA IP Rabbit Log in to see Polyclonal
0.0008946119 ABIN115684 EIA IP Rabbit Log in to see Polyclonal
0.0008946119 ABIN269286 ICC IF IP ELISA WB Mouse IgG1 Log in to see EWF
0.0008946119 ABIN1589705 IP ELISA WB Biotin Mouse IgG1 Log in to see EWI
0.0008946119 ABIN1502100 IP Mouse IgG1 Log in to see V12
0.0008946119 ABIN1502065 IP Mouse IgG1 Log in to see V12
0.0008946119 ABIN298980 IHC (fro) IP ELISA Mouse IgG Extracellular Domain Log in to see Flt-19
0.0008946119 ABIN1589685 IP ELISA WB Biotin Mouse IgG1 Log in to see EWF
0.0008946119 ABIN249298 IP ELISA Rabbit IgG Log in to see Polyclonal
0.0008946119 ABIN602536 IHC (fro) IP ELISA Mouse IgG Extracellular Domain Log in to see
0.0008946119 ABIN449642 IP ELISA WB Mouse IgG1 Log in to see Flt-1-EWI
0.0008946119 ABIN269287 IP ELISA Rabbit IgG Log in to see Polyclonal
0.0008946119 ABIN355006 IP ELISA WB Mouse IgG1 Log in to see
0.0008946119 ABIN355005 IP ELISA Rabbit IgG Log in to see Polyclonal

Top referenced anti-FLT1 antibody for Immunoprecipitation

Similar anti-FLT1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 29 Antibodies 118 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 12 Antibodies
Flow Cytometry (FACS) 2 Antibodies 27 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 22 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 43 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Dot Blot (DB) 3 Antibodies
Functional Studies (Func) 2 Antibodies

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope Extracellular Domain
(32), (20), (13), (10), (9), (9), (8), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(245), (74), (64), (12), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(189), (85), (7), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT1 antibody is un-conjugated
(15), (10), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(171), (132), (55), (47), (37), (25), (20), (16), (11), (11), (11), (5), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 Antibody (ABIN115152) Images
Specificity The monoclonal antibody will recognize recombinant and naturally occurring form of Human VEGFR-1/Flt-1.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Affinity Chromatography on Protein G
Immunogen Recombinant Human soluble extracellular Flt-1 protein (D5) (Cat.-No DA3539)
Clone FLTEWF
Isotype IgG1

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 Antibody (ABIN115152) Images back to top
Antigen
Alternative Name VEGFR-1 / Flt-1 (FLT1 Antibody Abstract)
Background Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA.Synonyms: FLT, FLT1, FRT, Fms-like tyrosine kinase 1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, VEGF Receptor 1, VEGFR1, Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor
Gene ID 2321
NCBI Accession NP_001153392
UniProt P17948
Research Area Cytokines, Receptors
Pathways RTK Signaling

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 Antibody (ABIN115152) Images back to top
Application Notes ELISA (1.5-8 μg/mL), Western blotting (1-10 μg/mL), Immunoprecipitation (1-5 μg/mL lysateor reaction volume).
Other applications not tested.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 Antibody (ABIN115152) Images back to top
Reconstitution Restore with distilled sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer PBS pH 7.4 containing no preservative
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-FLT1 Antibody (ABIN115152)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Background publications

Roeckl, Hecht, Sztajer, Waltenberger, Yayon, Weich: "Differential binding characteristics and cellular inhibition by soluble VEGF receptors 1 and 2." in: Experimental cell research, Vol. 241, Issue 1, pp. 161-70, 1998

Barleon, Totzke, Herzog, Blanke, Kremmer, Siemeister, Marmé, Martiny-Baron: "Mapping of the sites for ligand binding and receptor dimerization at the extracellular domain of the vascular endothelial growth factor receptor FLT-1." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10382-8, 1997

Chi, Hsieh, Hui, Tam: "Modified method for double stranded DNA sequencing and synthetic oligonucleotide purification." in: Nucleic acids research, Vol. 16, Issue 21, pp. 10382, 1989