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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||27 Antibodies||29 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||4 Antibodies||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||21 Antibodies||22 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||12 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies||10 Antibodies|
|Western Blotting (WB)||31 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Antigen||Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FLT1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-FLT1 AntibodyTarget Details FLT1 Application Details Handling Images
|Specificity||The antibody can detect both VEGFR1 and its splice isoform sFlt1.|
|Immunogen||A synthetic peptide corresponding to residues in N-terminus of human VEGFR1 was used as immunogen.|
Target Details FLT1Product Details anti-FLT1 Antibody Application Details Handling Images back to top
|Alternative Name||VEGFR-1 / Flt-1 (FLT1 Antibody Abstract)|
VEGFR-1 (vascular endothelial growth factor receptor 1) is a 180-kDa membranereceptor tyrosine kinase that is the receptor for vascular endothelial growth factor(VEGF), VEGFB and PGF. VEGF is a key regulator of physiological angiogenesisduring embryogenesis, skeletal growth and reproductive functions. VEGF has also beenimplicated in pathological angiogenesis associated with tumors, intraocular neovasculardisorders and other conditions (1). VEGFR-1 has seven extracellular Ig-like domains,the second domain from the N-terminus is necessary and sufficient for high affinityVEGF binding (2). The biological effects of VEGF are mediated by VEGFR-1 andVEGFR-2 (1), and two major tyrosine phosphorylation sites at Tyr 1213 and Tyr 1242and two minor tyrosine phosphorylation sites at Tyr 1327 and Tyr 1333 exist inVEGFR-1 (3). Alternate Names: anti-Flt 1 antibody, anti-FLT antibody, anti-Flt1 antibody, anti-Fms like tyrosine kinase 1antibody, anti-Fms related tyrosine kinase 1 antibody, anti-FRT antibody, anti-Tyrosineprotein kinase FRT antibody, anti-Tyrosine protein kinase receptor FLT antibody,anti-Vascular endothelial growth factor receptor 1 Precursor antibody, anti-Vascularpermeability factor receptor antibody, anti-VEGFR 1 antibody, anti-VEGFR1 antibody.
Gene Symbol: FLT1
|Gene ID||2321, 14254, 54251|
|Research Area||Cytokines, Receptors|
Application DetailsProduct Details anti-FLT1 Antibody Target Details FLT1 Handling Images back to top
In Western blot this antibody detects a band at approximately 180 kDa. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24647000)
Recommended dilutions: Flow Cytometry 1:1000, Immunocytochemistry/Immunofluorescence 1:250, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:250, Immunohistochemistry-Paraffin 1:250, Immunoprecipitation 1:100, Western Blot 1:500-1000
Protocol specific for VEGF Receptor 1 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-FLT1 Antibody Target Details FLT1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling back to top