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anti-Human FLT3 antibody for Flow Cytometry

Recommended FLT3 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
  • B230315G04
  • CD135
  • FLK-2
  • Flk-2
  • FLK2
  • Flk2
  • Flt-3
  • FLT3
  • Ly72
  • STK1
  • wmfl
Reactivity
Human
277
129
40
1
1
1
Host
Mouse
245
58
20
1
Clonality
Monoclonal
Conjugate
This FLT3 antibody is conjugated to FITC
20
18
15
13
10
6
4
4
4
4
4
4
4
4
4
4
2
1
1
Application
Flow Cytometry (FACS)
164
121
75
46
46
45
20
16
10
4
2
2
2
1
1
Supplier
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Catalog No. ABIN487500
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN487499 FACS Mouse IgG1 Log in to see BV10
0.30226976 ABIN2660938 FACS Biotin Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN2658914 FACS PE-Cy5 Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN2659088 FACS PE-Cy7 Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN2658477 FACS APC Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN2663011 FACS PE Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN2664204 FACS Mouse IgG1 kappa Log in to see BV10A4H2 4
0.30226976 ABIN1996824 FACS FITC Mouse IgG1 AA 1-541 Log in to see 2
0.30226976 ABIN2436919 FACS Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.30226976 ABIN2677506 FACS APC Mouse IgG1 Log in to see BV10A4H2
0.30226976 ABIN2677503 FACS PE Mouse IgG1 Log in to see BV10A4H2
0.30226976 ABIN2213921 FACS APC Mouse Log in to see
0.0010482287 ABIN391981 FACS IHC (p) WB Rabbit Ig AA 33-64, N-Term Log in to see Polyclonal 4
0.0010482287 ABIN614773 FACS IP Mouse IgG1 Log in to see BV10A4 5
0.0010482287 ABIN614774 FACS PE Mouse IgG1 Log in to see BV10A4 5
0.0010482287 ABIN489931 FACS IP PE Mouse IgG1 Log in to see BV10A4 5
0.0010482287 ABIN457417 FACS IP Mouse IgG1 Log in to see BV10A4 4
0.0010482287 ABIN625859 FACS IHC (p) Mouse IgG1 Log in to see BV10A4H2
0.0010482287 ABIN1302921 FACS IP FITC Mouse IgG1 Log in to see BV10A4 5
0.0010482287 ABIN616764 FACS IHC (p) Mouse IgG1 Log in to see BV10A4H2

Top referenced anti-FLT3 antibody for Flow Cytometry

Similar anti-FLT3 Antibodies

Application / Reactivity Human
ELISA 106 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 61 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 46 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Western Blotting (WB) 136 Antibodies

General

Antigen Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
Reactivity Human
(277), (129), (40), (1), (1), (1)
Host Mouse
(245), (58), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT3 antibody is conjugated to FITC
(20), (18), (15), (13), (10), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1)
Application Flow Cytometry (FACS)
(164), (121), (75), (46), (46), (45), (20), (16), (10), (4), (2), (2), (2), (1), (1)
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Product Details anti-FLT3 Antibody

Target Details FLT3 Application Details Handling Images
Specificity Recognizes CD135/FLT3.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: FL cytokine receptor, STK1, Tyrosine-protein kinase receptor FLT3, Stem cell tyrosine kinase1
Purification Protein-A Sepharose Chromatography of hybridoma supernatant.
Immunogen Pro B-cell line, BV-173. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2/0 with Balb/cmouse splenocyte.
Clone BV10
Isotype IgG1

Target Details FLT3

Product Details anti-FLT3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD135 / FLT3 (FLT3 Antibody Abstract)
Background CD135, also known as FLT3 or FLK2, is a receptor tyrosine kinase involved in the early steps of hematopoiesis. CD135 is expressed by immature hematopoietic cells and leukemia cells and is involved in the survival, proliferation, and differentiation of stem cells. Mutations in CD135 are also associated with the development of about one third of human acute myeloid leukemias (ALM) and B-lineage acute lymphocytic leukemias (ALL).Synonyms: FL cytokine receptor, STK1, Stem cell tyrosine kinase 1, Tyrosine-protein kinase receptor FLT3
Gene ID 2322
UniProt P36888
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways RTK Signaling

Application Details

Product Details anti-FLT3 Antibody Target Details FLT3 Handling Images back to top
Application Notes Flow Cytometry: 50 μg/mL (final concentration). Positive Control: THP-1 cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Flow cytometric analysis for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 µL of the primary antibody at the concentration of as suggest in theAPPLICATIONS diluted in the washing buffer. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control for Flow Cytometry: THP-1
Restrictions For Research Use only

Handling

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Images back to top
Concentration 0.05 mg/mL
Buffer PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FLT3 antibody (Fms-Related tyrosine Kinase 3)  (FITC) (ABIN487500) anti-Fms-Related tyrosine Kinase 3 (FLT3) antibody (FITC)