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anti-Mouse (Murine) FLT3 antibody for Flow Cytometry

Recommended FLT3 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
  • FLT3
  • B230315G04
  • CD135
  • Flk-2
  • Flk2
  • Flt-3
  • Ly72
  • wmfl
  • FLK-2
  • FLK2
  • STK1
  • fms-related tyrosine kinase 3
  • FMS-like tyrosine kinase 3
  • FLT3
  • Flt3
Reactivity
Mouse (Murine)
294
136
43
1
1
1
Host
Rat
256
64
18
4
1
Clonality
Monoclonal
Conjugate
This FLT3 antibody is conjugated to APC
20
20
16
10
10
6
4
4
4
4
4
4
4
4
4
4
4
3
2
2
1
1
1
Application
Blocking Reagent (BR), Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
170
121
81
54
49
45
21
16
11
4
2
1
1
1
1
Supplier
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Catalog No. ABIN2658479
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.113617 ABIN2663013 FACS PE Rat IgG2a kappa Log in to see A2F10 8
13.113617 ABIN2660812 FACS IP IHC Biotin Rat IgG2a kappa Log in to see A2F10 8
13.113617 ABIN4897165 FACS PE Rat IgG2a AA 28-544 Log in to see 113308
13.113617 ABIN4897164 FACS Allophycocyanin Rat IgG2a AA 28-544 Log in to see 113308
1 ABIN4900092 FACS WB Goat IgG AA 28-544 Log in to see Polyclonal 1
1 ABIN475837 FACS Cy5,PE Rat IgG2a, kappa Log in to see A2F10
1 ABIN474488 FACS PE Rat IgG2a, kappa Log in to see A2F10
1 ABIN475363 FACS IP Rat IgG2a, kappa Log in to see A2F10
1 ABIN4900091 FACS ICC Rat IgG2a AA 28-544 Log in to see 113308
1 ABIN2660811 FACS IP IHC Biotin Rat IgG2a kappa Log in to see A2F10 8
1 ABIN2658480 BR ICC FACS WB APC Rat IgG2a kappa Log in to see A2F10 7
1 ABIN2663014 FACS PE Rat IgG2a kappa Log in to see A2F10 8
1 ABIN474370 FACS Biotin Rat IgG2a, kappa Log in to see A2F10
1 ABIN4900090 FACS Biotin Rat IgG2a AA 28-544 Log in to see 113308
1 ABIN4900089 FACS WB Biotin Goat IgG AA 28-544 Log in to see Polyclonal

Top referenced anti-FLT3 antibody for Flow Cytometry

Similar anti-FLT3 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 2 Antibodies
ELISA 44 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 40 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 78 Antibodies

General

Antigen Fms-Related tyrosine Kinase 3 (FLT3) Antibodies
Reactivity Mouse (Murine)
(294), (136), (43), (1), (1), (1)
Host Rat
(256), (64), (18), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT3 antibody is conjugated to APC
(20), (20), (16), (10), (10), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (1), (1), (1)
Application Blocking Reagent (BR), Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
(170), (121), (81), (54), (49), (45), (21), (16), (11), (4), (2), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-FLT3 Antibody

Target Details FLT3 Application Details Handling References for anti-FLT3 Antibody (ABIN2658479) Images
Purification The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Clone A2F10
Isotype IgG2a kappa

Target Details FLT3

Product Details anti-FLT3 Antibody Application Details Handling References for anti-FLT3 Antibody (ABIN2658479) Images back to top
Antigen
Alternative Name CD135 (FLT3 Antibody Abstract)
Background CD135, also known as Flk-2, Flt3, and Ly-72, is a type III tyrosine kinase receptor. It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells.
Research Area CD Antigens
Pathways RTK Signaling

Application Details

Product Details anti-FLT3 Antibody Target Details FLT3 Handling References for anti-FLT3 Antibody (ABIN2658479) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details References for anti-FLT3 Antibody (ABIN2658479) Images back to top
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-FLT3 Antibody (ABIN2658479)

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling Images back to top
Background publications

Auffray, Fogg, Narni-Mancinelli, Senechal, Trouillet, Saederup, Leemput, Bigot, Campisi, Abitbol, Molina, Charo, Hume, Cumano, Lauvau, Geissmann: "CX3CR1+ CD115+ CD135+ common macrophage/DC precursors and the role of CX3CR1 in their response to inflammation." in: The Journal of experimental medicine, Vol. 206, Issue 3, pp. 595-606, 2009

Waskow, Liu, Darrasse-Jèze, Guermonprez, Ginhoux, Merad, Shengelia, Yao, Nussenzweig: "The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues." in: Nature immunology, Vol. 9, Issue 6, pp. 676-83, 2008

Sergejeva, Johansson, Malmhäll, Lötvall: "Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments." in: Blood, Vol. 103, Issue 4, pp. 1270-7, 2004

Ogawa, Sugawara, Kunisada, Sudo, Hayashi, Nishikawa, Kodama, Nishikawa: "Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse." in: Experimental hematology, Vol. 26, Issue 6, pp. 478-88, 1998

Veiby, Jacobsen, Cui, Lyman, Jacobsen: "The flt3 ligand promotes the survival of primitive hemopoietic progenitor cells with myeloid as well as B lymphoid potential. Suppression of apoptosis and counteraction by TNF-alpha and TGF-beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2953-60, 1996

Veiby, Lyman, Jacobsen: "Combined signaling through interleukin-7 receptors and flt3 but not c-kit potently and selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells." in: Blood, Vol. 88, Issue 4, pp. 1256-65, 1996

Matthews, Jordan, Wiegand, Pardoll, Lemischka: "A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations." in: Cell, Vol. 65, Issue 7, pp. 1143-52, 1991

Images

Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling References for anti-FLT3 Antibody (ABIN2658479) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FLT3 antibody (Fms-Related tyrosine Kinase 3)  (APC) (ABIN2658479) anti-Fms-Related tyrosine Kinase 3 (FLT3) antibody (APC)
Flow Cytometry (FACS) image for anti-FLT3 antibody (Fms-Related tyrosine Kinase 3)  (APC) (ABIN2658479) anti-Fms-Related tyrosine Kinase 3 (FLT3) antibody (APC) (Image 2)