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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||8 Antibodies||53 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies||40 Antibodies|
|Immunohistochemistry (IHC)||4 Antibodies||16 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies||12 Antibodies|
|Western Blotting (WB)||18 Antibodies|
|Blocking Reagent (BR)||2 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Flow Cytometry (FACS)||23 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies|
|Immunofluorescence (IF)||5 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||2 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Antigen||Fms-Related tyrosine Kinase 3 (FLT3) Antibodies|
|Epitope||AA 62-295 Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This FLT3 antibody is un-conjugated Alternatives|
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FLT3 AntibodyTarget Details FLT3 Application Details Handling Images
|Purpose||Rabbit IgG polyclonal antibody for Flt3 / CD135 detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Flt3 / CD135 detection. Tested with WB, IHC-P, Direct ELISA in Mouse,Rat.
Gene Name: FMS-like tyrosine kinase 3
Protein Name: Receptor-type tyrosine-protein kinase FLT3
|Purification||Immunogen affinity purified.|
|Immunogen||E. coli-derived mouse Flt3 / CD135 recombinant protein (Position: E62-E295).|
Target Details FLT3Product Details anti-FLT3 Antibody Application Details Handling Images back to top
|Alternative Name||Flt3 (FLT3 Antibody Abstract)|
Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow.
Synonyms: Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Tyrosine-protein kinase receptor flk-2, CD135, Flt3, Flk-2, Flt-3
Application DetailsProduct Details anti-FLT3 Antibody Target Details FLT3 Handling Images back to top
|Application Notes||Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.|
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-FLT3 Antibody Target Details FLT3 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.