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anti-Human FLT4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended FLT4 Antibody (supplied by: Log in to see )

Antigen
Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
  • 6130401C07
  • AI323512
  • cb1059
  • Chy
  • Flk-1
  • Flk1
  • Flt-4
  • FLT41
  • Krd-1
  • LMPH1A
  • Ly73
  • PCL
  • sVEGFR-2
  • VEGFR-2
  • VEGFR-3
  • VEGFR2
  • Vegfr3
  • VEGFR3
Epitope
Extracellular Domain
35
14
14
13
12
9
9
6
5
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
158
76
31
2
1
Host
Mouse
120
60
17
4
1
Clonality
Monoclonal
Conjugate
This FLT4 antibody is un-conjugated
13
7
6
6
4
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
113
83
55
30
27
22
20
11
10
8
4
3
3
2
1
Supplier
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Catalog No. ABIN115161
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN567981 EIA IHC (fro) IHC (p) WB Mouse IgG1 Extracellular Domain Log in to see 2-00E-011 1
0.0008982679 ABIN115450 IHC (p) Rabbit C-Term Log in to see Polyclonal 1
0.0008982679 ABIN115449 IHC (fro) IHC (p) Rabbit C-Term Log in to see Polyclonal 2
0.0008982679 ABIN356407 EIA IHC (p) WB Mouse IgG1 Log in to see 44CT92-4-4
0.0008982679 ABIN678578 IF (p) IHC (p) WB Rabbit IgG AA 910-960 Log in to see Polyclonal 2
0.0008982679 ABIN266201 IHC (p) IHC Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN1688319 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN737406 IF (p) IHC (p) WB Rabbit IgG AA 920-970 Log in to see Polyclonal 1
0.0008982679 ABIN1688313 IHC (p) Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN790496 IHC (fro) FACS IHC (p) IP ELISA WB Mouse IgG1 Extracellular Domain Log in to see 9D9F9
0.0008982679 ABIN258821 IHC (p) IHC WB Mouse IgG1 N-Term Log in to see MM0003-7G63
0.0008982679 ABIN1492009 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN373447 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN678580 IHC (p) WB Biotin Rabbit IgG AA 910-960 Log in to see Polyclonal
0.0008982679 ABIN678587 IHC (p) WB HRP Rabbit IgG AA 910-960 Log in to see Polyclonal
0.0008982679 ABIN737408 IHC (p) WB Biotin Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0008982679 ABIN737415 IHC (p) WB HRP Rabbit IgG AA 920-970 Log in to see Polyclonal
0.0008982679 ABIN1960107 IHC (p) WB Mouse IgG2a Log in to see 818CT12-1-1
0.0008982679 ABIN1804664 IHC (p) ELISA Rabbit Log in to see Polyclonal
0.0008982679 ABIN1840042 IHC (p) WB Mouse IgG1, kappa Log in to see 44CT92-4-4

Top referenced anti-FLT4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-FLT4 Antibodies

Application / Reactivity Human
ELISA 69 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 50 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 100 Antibodies

General

Antigen Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
Epitope Extracellular Domain
(35), (14), (14), (13), (12), (9), (9), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (76), (31), (2), (1)
Host Mouse
(120), (60), (17), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT4 antibody is un-conjugated
(13), (7), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(113), (83), (55), (30), (27), (22), (20), (11), (10), (8), (4), (3), (3), (2), (1)
Pubmed 2 references available
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Product Details anti-FLT4 Antibody

Target Details FLT4 Application Details Handling References for anti-FLT4 Antibody (ABIN115161) Images
Specificity Detects Human VEGFR-3/FLT-4.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen Purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.
Clone (3C3) 9D9
Isotype IgG1

Target Details FLT4

Product Details anti-FLT4 Antibody Application Details Handling References for anti-FLT4 Antibody (ABIN115161) Images back to top
Antigen
Alternative Name VEGFR-3 / Flt-4 (FLT4 Antibody Abstract)
Background VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.Synonyms: FLT4, Tyrosine-protein kinase receptor FLT4, VEGF Receptor 3, VEGFR3, Vascular endothelial growth factor receptor 3
Gene ID 2324
NCBI Accession NP_002011
UniProt P35916
Research Area Cytokines, Receptors
Pathways RTK Signaling

Application Details

Product Details anti-FLT4 Antibody Target Details FLT4 Handling References for anti-FLT4 Antibody (ABIN115161) Images back to top
Application Notes ELISA: 0.5-1.5 μg/mL in direct ELISA, allows detection of 0.25-0.5 ng/well. Immunohistochemistry: 1-10 μg/mL. Western blot 0.5-1 μg/mL, detection limit is approximately 5 ng/lane under reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details References for anti-FLT4 Antibody (ABIN115161) Images back to top
Reconstitution Restore with sterile water to a concentration of 0.1-1.0 mg/mL
Buffer PBS pH 7.4 without preservatives or stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8 °C or at -20 °C for longer. Aliquot to
Expiry Date 12 months

References for anti-FLT4 Antibody (ABIN115161)

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling Images back to top
Product cited in:

Davids, Davids, Charlton, Ng, Chong, Laubscher, Dar, Hodge, Soong, Goh: "Response to a novel multitargeted tyrosine kinase inhibitor pazopanib in metastatic Merkel cell carcinoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 26, pp. e97-100, 2009

Background publications

Jussila, Valtola, Partanen, Salven, Heikkilä, Matikainen, Renkonen, Kaipainen, Detmar, Tschachler, Alitalo, Alitalo: "Lymphatic endothelium and Kaposi's sarcoma spindle cells detected by antibodies against the vascular endothelial growth factor receptor-3." in: Cancer research, Vol. 58, Issue 8, pp. 1599-604, 1998

Images

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling References for anti-FLT4 Antibody (ABIN115161) back to top
Supplier Images
Western Blotting (WB) image for anti-FLT4 antibody (Fms-Related Tyrosine Kinase 4) (Extracellular Domain) (ABIN115161) Western analysis (AP) using recombinant human soluble VEGFR-3/FLT-4 as target!