anti-Human FLT4 antibody for Western Blotting

Recommended FLT4 Antibody (supplied by: Log in to see )

Antigen
Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
  • AI323512
  • Chy
  • Flt-4
  • VEGFR-3
  • VEGFR3
  • FLT41
  • LMPH1A
  • PCL
  • Vegfr3
  • cb1059
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • FMS-like tyrosine kinase 4
  • fms-related tyrosine kinase 4
  • kinase insert domain protein receptor
  • Flt4
  • FLT4
  • flt4
  • Kdr
Epitope
Extracellular Domain
30
21
16
15
14
12
10
9
9
7
6
6
4
3
2
1
1
1
1
1
1
Reactivity
Human
198
130
46
Host
Mouse
125
93
48
9
1
Clonality
Monoclonal
Conjugate
This FLT4 antibody is un-conjugated
19
10
8
6
6
6
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
174
106
91
67
65
27
26
23
11
8
6
5
3
3
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN115161
$ 412.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN616061 WB Rabbit AA 1279-1283 Log in to see Polyclonal 3
3.3487515 ABIN567981 EIA IHC (fro) IHC (p) WB Mouse IgG1 Extracellular Domain Log in to see 2-00E-011 1
3.3487515 ABIN356407 EIA IHC (p) WB Mouse IgG1 Log in to see 44CT92-4-4
3.3487515 ABIN1997515 ELISA WB Rabbit IgG AA 1-776, Extracellular Domain Log in to see Polyclonal 2
3.3487515 ABIN1882290 WB Mouse IgG2a Log in to see 818CT12-1-1 4
3.3487515 ABIN969147 ELISA WB Mouse IgG1 Log in to see 4H4 3
3.3487515 ABIN1109477 EIA WB Mouse IgG1 Log in to see 4H4 2
3.3487515 ABIN3197840 IHC WB Rabbit Log in to see Polyclonal
3.3487515 ABIN4251321 WB Mouse IgG1 Log in to see 1G2
3.3487515 ABIN449654 IP ELISA WB Rat IgG1 Log in to see 7A3
3.3487515 ABIN449652 CyTOF ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 9D9
3.3487515 ABIN3223014 IHC WB Rabbit Log in to see Polyclonal
3.3487515 ABIN1876806 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN4251307 ELISA WB Mouse IgG1 Log in to see 4H4
3.3487515 ABIN560929 ELISA WB Mouse IgG2a kappa AA 34-133, partial Log in to see 5B5
3.3487515 ABIN3030977 FACS IHC ELISA WB Mouse IgG2a Log in to see 818CT12-1-1
3.3487515 ABIN931500 ELISA WB Mouse IgG1 Log in to see 4H4
3.3487515 ABIN1846209 ELISA WB Mouse IgG1 Log in to see
1 ABIN678578 FACS IF (p) IHC (p) WB Rabbit IgG AA 910-960 Log in to see Polyclonal 3
1 ABIN115160 EIA WB Biotin Mouse IgG1 Extracellular Domain Log in to see 3C3 2

Top referenced anti-FLT4 antibody for Western Blotting

Similar anti-FLT4 Antibodies

Application / Reactivity Human
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 74 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 66 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 47 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Western Blotting (WB) 122 Antibodies

General

Antigen Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies
Epitope Extracellular Domain
(30), (21), (16), (15), (14), (12), (10), (9), (9), (7), (6), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(198), (130), (46)
Host Mouse
(125), (93), (48), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FLT4 antibody is un-conjugated
(19), (10), (8), (6), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(174), (106), (91), (67), (65), (27), (26), (23), (11), (8), (6), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FLT4 Antibody

Target Details FLT4 Application Details Handling ProductDetails: References for anti-FLT4 antibody (ABIN115161) Images
Specificity Detects Human VEGFR-3/FLT-4.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G.
Immunogen Purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.
Clone (3C3) 9D9
Isotype IgG1

Target Details FLT4

Product Details anti-FLT4 Antibody Application Details Handling ProductDetails: References for anti-FLT4 antibody (ABIN115161) Images back to top
Antigen
Alternative Name VEGFR-3 / Flt-4 (FLT4 Antibody Abstract)
Background VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.Synonyms: FLT4, Tyrosine-protein kinase receptor FLT4, VEGF Receptor 3, VEGFR3, Vascular endothelial growth factor receptor 3
Gene ID 2324
NCBI Accession NP_002011
UniProt P35916
Research Area Cytokines, Receptors
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

Product Details anti-FLT4 Antibody Target Details FLT4 Handling ProductDetails: References for anti-FLT4 antibody (ABIN115161) Images back to top
Application Notes ELISA: 0.5-1.5 μg/mL in direct ELISA, allows detection of 0.25-0.5 ng/well. Immunohistochemistry: 1-10 μg/mL. Western blot 0.5-1 μg/mL, detection limit is approximately 5 ng/lane under reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details ProductDetails: References for anti-FLT4 antibody (ABIN115161) Images back to top
Reconstitution Restore with sterile water to a concentration of 0.1-1.0 mg/mL
Buffer PBS pH 7.4 without preservatives or stabilizers
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8 °C or at -20 °C for longer. Aliquot to
Expiry Date 12 months

ProductDetails: References for anti-FLT4 antibody (ABIN115161)

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling Images back to top
Product cited in:

Davids, Davids, Charlton, Ng, Chong, Laubscher, Dar, Hodge, Soong, Goh: "Response to a novel multitargeted tyrosine kinase inhibitor pazopanib in metastatic Merkel cell carcinoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 26, pp. e97-100, 2009

Background publications

Jussila, Valtola, Partanen, Salven, Heikkilä, Matikainen, Renkonen, Kaipainen, Detmar, Tschachler, Alitalo, Alitalo: "Lymphatic endothelium and Kaposi's sarcoma spindle cells detected by antibodies against the vascular endothelial growth factor receptor-3." in: Cancer research, Vol. 58, Issue 8, pp. 1599-604, 1998

Images

Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling ProductDetails: References for anti-FLT4 antibody (ABIN115161) back to top
Supplier Images
Western Blotting (WB) image for anti-FLT4 antibody (Fms-Related Tyrosine Kinase 4) (Extracellular Domain) (ABIN115161) Western analysis (AP) using recombinant human soluble VEGFR-3/FLT-4 as target!