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Mouse (Murine) FLT4 ELISA Kit

Recommended FLT4 Product (supplied by: Log in to see )

Antigen
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kits
  • 6130401C07
  • AI323512
  • cb1059
  • Chy
  • Flk-1
  • Flk1
  • Flt-4
  • FLT41
  • Krd-1
  • LMPH1A
  • Ly73
  • PCL
  • sVEGFR-2
  • VEGFR-2
  • VEGFR-3
  • VEGFR2
  • Vegfr3
  • VEGFR3
Epitope
AA 25-770
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
15
11
6
2
2
1
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
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Catalog No. ABIN2859280
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012117105 ABIN367721 Sandwich ELISA Serum, Plasma, Tissue Homogenate 9.38-600 pg/mL 9.38 pg/mL Log in to see
0.0012117105 ABIN773946 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids Log in to see
0.0012117105 ABIN2703571 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.2-50 ng/mL 0.2 ng/mL Log in to see
0.0012117105 ABIN2748920 Sandwich ELISA Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN2684294 Sandwich ELISA Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
0.0012117105 ABIN456774 Log in to see

Top referenced FLT4 ELISA Kit for ELISA

  • Mouse (Murine) FLT4 ELISA Kit for Sandwich ELISA - ABIN2859280 (1 Publications): Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma. in PLoS ONE 2013 (PubMed)

Similar FLT4 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 7 ELISA Kits

General

Antigen Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kits
Epitope AA 25-770
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(15), (11), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details FLT4 ELISA Kit

Target details Application Details Handling References for FLT4 Kit (ABIN2859280) Images
Purpose For quantitative detection of mouse VEGFR3 in cell culture supernates and serum.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Detection Method Colorimetric
Specificity Natural and recombinant mouse VEGFR3
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse VEGFR3 antibod - 1
  • Lyophilized recombinant mouse VEGFR3 standar - 10ng/tubex2
  • Biotinylated anti- mouse VEGFR3 antibod - 130ul(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex(ABC - 130ul(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: NSO
Immunogen sequence: Y25-D770

Target details

Product Details FLT4 ELISA Kit Application Details Handling References for FLT4 Kit (ABIN2859280) Images back to top
Antigen
Alternative Name FLT4 (FLT4 ELISA Kit Abstract)
Background Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
Gene ID 14257
UniProt P35917
Pathways RTK Signaling

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse VEGFR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGFR3 has been precoated onto 96-well plates. Standards(NSO, Y25-D770) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGFR3 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse VEGFR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse VEGFR3 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.057, CV(%): 4.3
  • Sample 2: n=16, Mean(ng/ml): 3.75, Standard deviation: 0.191, CV(%): 5.1
  • Sample 3: n=16, Mean(ng/ml): 6.04, Standard deviation: 0.344, CV(%): 5.7,
  • Sample 1: n=24, Mean(ng/ml): 1.57, Standard deviation: 0.082, CV(%): 5.2
  • Sample 2: n=24, Mean(ng/ml): 4.03, Standard deviation: 0.258, CV(%): 6.4
  • Sample 3: n=24, Mean(ng/ml): 6.49, Standard deviation: 0.474, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details FLT4 ELISA Kit Target details Application Details References for FLT4 Kit (ABIN2859280) Images back to top
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FLT4 Kit (ABIN2859280)

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Product cited in:

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, 2013

Images

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Supplier Images
ELISA image for FLT4 ELISA Kit (Fms-Related Tyrosine Kinase 4) (ABIN2859280) Mouse VEGFR3/FLT4 PicoKine ELISA Kit standard curve