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Mouse (Murine) FLT4 ELISA Kit

Recommended FLT4 Product (supplied by: Log in to see )

Antigen
Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kits
  • AI323512
  • Chy
  • Flt-4
  • VEGFR-3
  • VEGFR3
  • FLT41
  • LMPH1A
  • PCL
  • Vegfr3
  • cb1059
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • FMS-like tyrosine kinase 4
  • fms-related tyrosine kinase 4
  • kinase insert domain protein receptor
  • Flt4
  • FLT4
  • flt4
  • Kdr
Epitope
AA 25-770
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
21
12
6
3
1
1
1
Method Type
Sandwich ELISA
Detection Range
156-10000 pg/mL
Minimum Detection Limit
156 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2859280
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.917189 ABIN2703571 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.2-50 ng/mL 0.2 ng/mL Log in to see
1 ABIN367721 Sandwich ELISA Serum, Plasma, Tissue Homogenate 9.38-600 pg/mL 9.38 pg/mL Log in to see
1 ABIN5523681 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 62.5-4000 pg/mL 62.5 pg/mL Log in to see
1 ABIN2748920 Sandwich ELISA Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN2684294 Sandwich ELISA Cell Culture Supernatant, Serum 156-10000 pg/mL 156 pg/mL Log in to see
1 ABIN456774 Log in to see

Top referenced FLT4 ELISA Kit for ELISA

  • Mouse (Murine) FLT4 ELISA Kit for Sandwich ELISA - ABIN2859280 (1 Publications): Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma. in PLoS ONE 2013 (PubMed)

Similar FLT4 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 7 ELISA Kits

General

Antigen Fms-Related Tyrosine Kinase 4 (FLT4) ELISA Kits
Epitope AA 25-770
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(21), (12), (6), (3), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 156-10000 pg/mL
Minimum Detection Limit 156 pg/mL
Application ELISA
Pubmed 1 reference available
Supplier Log in to see

Product Details FLT4 ELISA Kit

Target details Application Details Handling References for FLT4 Kit (ABIN2859280) Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse VEGFR3/FLT4
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: Y25-D770
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Y25-D770

Target details

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Antigen
Alternative Name FLT4 (FLT4 ELISA Kit Abstract)
Background

Protein Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473'. .

Background: Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.

Synonyms: Vascular endothelial growth factor receptor 3,VEGFR-3,2.7.10.1,Fms-like tyrosine kinase 4,FLT-4,Tyrosine-protein kinase receptor FLT4,Flt4,Flt-4, Vegfr3,

Full Gene Name: Vascular endothelial growth factor receptor 3

Cellular Localisation: Cell membrane, Single-pass type I membrane protein . Cytoplasm . Nucleus . Ligand-mediated autophosphorylation leads to rapid internalization.
Gene ID 14257
UniProt P35917
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Tissue Specificity: Expressed in adult lung and liver, and in fetal liver, brain, intestine and placenta. .

Plate Pre-coated,Strips (12 x 8)
Protocol mouse VEGFR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGFR3 has been precoated onto 96-well plates. Standards(NSO, Y25-D770) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGFR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGFR3 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse VEGFR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse VEGFR3 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 1.32, Standard deviation: 0.057, CV(%): 4.3
  • Sample 2: n=16, Mean(ng/ml): 3.75, Standard deviation: 0.191, CV(%): 5.1
  • Sample 3: n=16, Mean(ng/ml): 6.04, Standard deviation: 0.344, CV(%): 5.7,
  • Sample 1: n=24, Mean(ng/ml): 1.57, Standard deviation: 0.082, CV(%): 5.2
  • Sample 2: n=24, Mean(ng/ml): 4.03, Standard deviation: 0.258, CV(%): 6.4
  • Sample 3: n=24, Mean(ng/ml): 6.49, Standard deviation: 0.474, CV(%): 7.3
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FLT4 Kit (ABIN2859280)

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Product cited in:

Li, Fan, Song, Zhang, Chen, Li, Mi, Ma, Song, Tao, Li: "Expression of angiopoietin-2 and vascular endothelial growth factor receptor-3 correlates with lymphangiogenesis and angiogenesis and affects survival of oral squamous cell carcinoma." in: PLoS ONE, Vol. 8, Issue 9, pp. e75388, 2013

Images

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Supplier Images
ELISA image for FLT4 ELISA Kit (Fms-Related Tyrosine Kinase 4) (ABIN2859280) Mouse VEGFR3/FLT4 PicoKine ELISA Kit standard curve