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Mouse (Murine) GAS6 ELISA Kit

Recommended GAS6 Product (supplied by: Log in to see )

Antigen
Growth Arrest-Specific 6 (GAS6) ELISA Kits
  • AXLLG
  • AXSF
  • fj34a12
  • Gas-6
  • GAS6
  • gas6
  • MGC68463
  • MGC83872
  • wu:fj34a12
  • zgc:63860
Epitope
AA 115-673
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
11
10
5
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889367
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN415796 Sandwich ELISA Serum, Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.32693866 ABIN1569983 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
0.32693866 ABIN2708514 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN1884548 Sandwich ELISA 78.13-5000 pg/mL 78.13 pg/mL Log in to see
0.0012117105 ABIN625395 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant Log in to see
0.0012117105 ABIN2684337 Sandwich ELISA Plasma (EDTA), Plasma (heparin), Cell Culture Supernatant, Serum 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.0012117105 ABIN2762218 Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.0012117105 ABIN2684336 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012117105 ABIN2534120 Sandwich ELISA Serum, Plasma, Biological Fluids 1.56-100 ng/mL 1.56 ng/mL Log in to see
0.0012117105 ABIN2948037 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012117105 ABIN1573767 Log in to see

Top referenced GAS6 ELISA Kit for ELISA

  • Mouse (Murine) GAS6 ELISA Kit for Sandwich ELISA - ABIN1889367 (2 Publications): Angelillo-Scherrer, de Frutos, Aparicio, Melis, Savi, Lupu, Arnout, Dewerchin, Hoylaerts, Herbert, Collen, Dahlbäck, Carmeliet: Deficiency or inhibition of Gas6 causes platelet dysfunction and protects mice against thrombosis. in Nature medicine 2001 (PubMed)

Similar GAS6 ELISA Kits

Application / Reactivity Mouse (Murine)
ELISA 12 ELISA Kits

General

Antigen Growth Arrest-Specific 6 (GAS6) ELISA Kits
Epitope AA 115-673
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(11), (10), (5), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 2 references available
Supplier Log in to see

Product Details GAS6 ELISA Kit

Target details Application Details Handling References for GAS6 Kit (ABIN1889367) Images
Purpose For quantitative detection of mouse GAS6 in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant mouse GAS6
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- mouse GAS6 antibod - 1
  • Lyophilized recombinant mouse GAS6 standar - 10ng/tubex2
  • Biotinylated anti- mouse GAS6 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: D115-P673

Target details

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Antigen
Alternative Name GAS6 (GAS6 ELISA Kit Abstract)
Background GAS6(Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid(Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.
Gene ID 14456
UniProt Q61592
Pathways RTK Signaling

Application Details

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Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol mouse GAS6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for GAS6 has been precoated onto 96-well plates. Standards(NSO, D115-P673) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GAS6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse GAS6 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse GAS6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse GAS6 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 156, Standard deviation: 9.048, CV(%): 5.8
  • Sample 2: n=16, Mean(pg/ml): 724, Standard deviation: 34.03, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 1345, Standard deviation: 55.15, CV(%): 4.1,
  • Sample 1: n=24, Mean(pg/ml): 174 , Standard deviation: 11.31, CV(%): 6.5
  • Sample 2: n=24, Mean(pg/ml): 617, Standard deviation: 33.32, CV(%): 5.4
  • Sample 3: n=24, Mean(pg/ml): 1436, Standard deviation: 112.01, CV(%): 7.8
Restrictions For Research Use only

Handling

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Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for GAS6 Kit (ABIN1889367)

Product Details GAS6 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Yanagita, Ishimoto, Arai, Nagai, Ito, Nakano, Salant, Fukatsu, Doi, Kita: "Essential role of Gas6 for glomerular injury in nephrotoxic nephritis." in: The Journal of clinical investigation, Vol. 110, Issue 2, pp. 239-46, 2002

Angelillo-Scherrer, de Frutos, Aparicio, Melis, Savi, Lupu, Arnout, Dewerchin, Hoylaerts, Herbert, Collen, Dahlbäck, Carmeliet: "Deficiency or inhibition of Gas6 causes platelet dysfunction and protects mice against thrombosis." in: Nature medicine, Vol. 7, Issue 2, pp. 215-21, 2001

Images

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Supplier Images
ELISA image for GAS6 ELISA Kit (Growth Arrest-Specific 6) (ABIN1889367) Mouse GAS6 PicoKine ELISA Kit standard curve