anti-Cow (Bovine) GRB2 antibody for Enzyme Immunoassay

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
C-Term
33
16
10
8
7
6
6
6
5
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
160
63
63
15
9
9
7
5
4
4
3
3
3
3
3
2
2
1
1
Host
Goat
108
27
24
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
8
4
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
144
70
55
30
13
7
5
3
2
2
2
1
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Catalog No. ABIN5552700
$ 375.38
Plus shipping costs $45.00

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(33), (16), (10), (8), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(160), (63), (63), (15), (9), (9), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Host Goat
(108), (27), (24), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(8), (4), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(144), (70), (55), (30), (13), (7), (5), (3), (2), (2), (2), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Sequence C-PRNYVTPVNR NV
Specificity This antibody detects GRB2 / ASH at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Canine (Dog), Cow.
Species reactivity (tested):Human, Mouse
Purification Immunoaffinity Chromatography
Immunogen Synthetic peptide from C-Terminus of the protein sequence according to NP_002077.1, NP_987102.1 Genename: GRB2

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 / ASH (GRB2 Antibody Abstract)
Background Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.Synonyms: Growth factor receptor-bound protein 2
Gene ID 2885
NCBI Accession NP_002077
UniProt P62993
Research Area Signaling
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 30 kDa band seen in Jurkat lysate. Immunohistochemistry: 0.1-0.3 μg/mL. In paraffin embedded Human Tonsil, stains mantlezone and to a lessser extent germinal centre.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.