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anti-Human GRB2 antibody for Enzyme Immunoassay

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • AA408164
  • ASH
  • Ash
  • EGFRBP-GRB2
  • GRB2
  • grb2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
Epitope
Middle Region
35
18
15
7
6
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
148
42
42
10
8
3
3
2
2
2
2
1
1
Host
Rabbit
98
29
22
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
5
4
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
129
65
53
29
9
8
4
3
3
2
1
Supplier
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Catalog No. ABIN358795
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088338397 ABIN358793 EIA IHC (p) WB Rabbit Ig Tyr209 Log in to see Polyclonal 3
0.00088338397 ABIN782889 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal 1
0.00088338397 ABIN374150 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal 1
0.00088338397 ABIN615406 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal

Top referenced anti-GRB2 antibody for Enzyme Immunoassay

Similar anti-GRB2 Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 11 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 129 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope Middle Region
(35), (18), (15), (7), (6), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(148), (42), (42), (10), (8), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(98), (29), (22), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(5), (4), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(129), (65), (53), (29), (9), (8), (4), (3), (3), (2), (1)
Pubmed 3 references available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN358795) Images
Specificity This antibody reacts to GRB2.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human GRB2.
Isotype Ig

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 Antibody (ABIN358795) Images back to top
Antigen
Alternative Name GRB2 / ASH (GRB2 Antibody Abstract)
Background GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences.Synonyms: Growth factor receptor-bound protein 2
Gene ID 2885, 9606
UniProt P62993
Research Area Signaling
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 Antibody (ABIN358795) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 Antibody (ABIN358795) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-GRB2 Antibody (ABIN358795)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Kondo, Hirayama, Sugito, Shono, Tanaka, Kitamura: "Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation." in: The Journal of biological chemistry, Vol. 283, Issue 3, pp. 1428-36, 2008

Morimatsu, Takagi, Ota, Iwamoto, Yanagida, Sako: "Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 46, pp. 18013-8, 2007

Martínez, García-Domínguez, Domingo, Oliva, Zarich, Sánchez, Gutiérrez-Eisman, Llopis, Rojas: "Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction." in: Cellular signalling, Vol. 19, Issue 11, pp. 2277-85, 2007

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 Antibody (ABIN358795) back to top
Supplier Images
 image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (Middle Region) (ABIN358795) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (Middle Region) antibody
 image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (Middle Region) (ABIN358795) anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (Middle Region) antibody (Image 2)