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|Antigen||Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies|
|Epitope||Middle Region Alternatives|
|Conjugate||This GRB2 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-GRB2 AntibodyTarget Details GRB2 Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN358795) Images
|Specificity||This antibody reacts to GRB2.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human GRB2.|
Target Details GRB2Product Details anti-GRB2 Antibody Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN358795) Images back to top
|Alternative Name||GRB2 / ASH (GRB2 Antibody Abstract)|
|Background||GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences.Synonyms: Growth factor receptor-bound protein 2|
|Gene ID||2885, 9606|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation|
Application DetailsProduct Details anti-GRB2 Antibody Target Details GRB2 Handling ProductDetails: References for anti-GRB2 antibody (ABIN358795) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details ProductDetails: References for anti-GRB2 antibody (ABIN358795) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
ProductDetails: References for anti-GRB2 antibody (ABIN358795)Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Kondo, Hirayama, Sugito, Shono, Tanaka, Kitamura: "Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation." in: The Journal of biological chemistry, Vol. 283, Issue 3, pp. 1428-36, 2008
Morimatsu, Takagi, Ota, Iwamoto, Yanagida, Sako: "Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 46, pp. 18013-8, 2007
Martínez, García-Domínguez, Domingo, Oliva, Zarich, Sánchez, Gutiérrez-Eisman, Llopis, Rojas: "Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction." in: Cellular signalling, Vol. 19, Issue 11, pp. 2277-85, 2007
ImagesProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling ProductDetails: References for anti-GRB2 antibody (ABIN358795) back to top