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anti-Human GRB2 antibody for Immunohistochemistry (Frozen Sections)

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
AA 54-62
34
18
16
7
6
6
6
5
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
158
64
64
16
10
9
7
5
4
4
3
3
3
3
3
2
2
1
1
Host
Mouse
105
27
25
2
Clonality
Monoclonal
Conjugate
This GRB2 antibody is un-conjugated
8
4
3
3
3
3
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
141
69
55
31
12
7
5
4
2
2
1
1
Supplier
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Catalog No. ABIN208556
$ 881.83
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN330571 IHC (fro) IHC (p) Mouse IgG1, kappa Log in to see

Similar anti-GRB2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 141 Antibodies 57 Antibodies 57 Antibodies 14 Antibodies 2 Antibodies
Immunoprecipitation (IP) 7 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies 9 Antibodies 14 Antibodies 14 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 14 Antibodies 13 Antibodies 1 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope AA 54-62
(34), (18), (16), (7), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis
(158), (64), (64), (16), (10), (9), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(105), (27), (25), (2)
Clonality
Monoclonal
Conjugate This GRB2 antibody is un-conjugated
(8), (4), (3), (3), (3), (3)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(141), (69), (55), (31), (12), (7), (5), (4), (2), (2), (1), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Specificity Recognizes a protein with Mr24-25kD, identified as Grb2 (growth-factor receptor-bound protein 2), also known as ASH (abundant Src homology). Grb2 deletion studies indicate that the epitope for this antibody maps to amino acid residues 54-62. No cross-reactivity with other SH2/SH3 signaling intermediates. Species Reactivity: Human, bovine, mouse, rat and Xenopus.
Purification Protein G purified
Immunogen Purified recombinant human Grb2 protein.

Type of Immunogen: Recombinant protein
Isotype IgG1, kappa

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Name/Gene ID: GRB2

Synonyms: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, EGFRBP-GRB2, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2, Grb3-3, HT027, NCKAP2
Gene ID 2885
UniProt P62993
Research Area Cancer
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Approved: IF (1 μg/mL), IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Immunofluorescence: 1.0 μg/mL with an incubation of 2 hours at RT. Immunohistochemistry: 1-2 μg/mL with an incubation of 30 minutes at RT. (on acetone-fixed frozen and formalin-fixed/paraffin-embedded tissue sections. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.). Positive control: BALB 3T3 or NIH 3T3 cells, kidney or tonsil.
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4, 0.2 % BSA, 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Long term: Add glycerol (40-50%) -20°C
Short term: +4°C