anti-Human GRB2 antibody for Western Blotting

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • GRB2
  • grb2
  • AA408164
  • Ash
  • ASH
  • EGFRBP-GRB2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
  • growth factor receptor-bound protein 2
  • Growth factor receptor-bound protein 2
  • growth factor receptor bound protein 2
  • growth factor receptor-bound protein 2b
  • GRB2
  • grb2
  • Grb2
  • LOC100342500
  • grb2b
Epitope
C-Term
33
16
10
8
7
6
6
6
5
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
160
64
63
16
10
9
7
5
4
4
3
3
3
3
3
2
2
1
1
Host
Goat
108
27
24
2
Clonality
Polyclonal
Conjugate
This GRB2 antibody is un-conjugated
8
4
3
3
3
3
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
144
69
54
31
13
7
5
4
2
2
2
1
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Catalog No. ABIN184670
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1474795 ABIN2787701 WB Rabbit N-Term Log in to see Polyclonal
3.1474795 ABIN1532105 IHC ELISA WB Rabbit IgG AA 125-174, pSer159 Log in to see Polyclonal 2
3.1474795 ABIN1534949 ELISA WB Rabbit IgG AA 141-190 Log in to see Polyclonal 2
3.1474795 ABIN967754 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
3.1474795 ABIN967755 BI IP IHC WB Mouse IgG1 AA 1-217 Log in to see 81-GRB2 5
3.1474795 ABIN2853618 WB Mouse IgG1 N-Term Log in to see 3F2 6
3.1474795 ABIN390236 IHC (p) WB Rabbit Ig AA 187-216, Tyr209 Log in to see Polyclonal 3
3.1474795 ABIN390238 IHC (p) WB Rabbit Ig AA 89-118, Center Log in to see Polyclonal 2
3.1474795 ABIN257708 ELISA IHC IHC (p) WB Goat C-Term, both Isoforms Log in to see Polyclonal 1
3.1474795 ABIN3044446 WB Rabbit IgG AA 184-201, C-Term Log in to see Polyclonal
3.1474795 ABIN615406 EIA IHC (p) WB Goat C-Term Log in to see Polyclonal
3.1474795 ABIN2854697 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.1474795 ABIN2854311 IHC (p) WB Rabbit IgG full length Log in to see Polyclonal
3.1474795 ABIN499082 WB Rabbit Log in to see Polyclonal
3.1474795 ABIN3184932 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1474795 ABIN302091 WB Rabbit IgG C-Term, AA 198-217 Log in to see Polyclonal 2
3.1474795 ABIN615471 IHC (p) WB Rabbit Log in to see Polyclonal
3.1474795 ABIN2465225 IHC ELISA WB Goat C-Term Log in to see Polyclonal
3.1474795 ABIN5609789 ELISA WB Biotin Goat C-Term Log in to see Polyclonal
3.1474795 ABIN1872885 IF WB Rabbit IgG Log in to see Polyclonal

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(33), (16), (10), (8), (7), (6), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(160), (64), (63), (16), (10), (9), (7), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1)
Host Goat
(108), (27), (24), (2)
Clonality
Polyclonal
Conjugate This GRB2 antibody is un-conjugated
(8), (4), (3), (3), (3), (3)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(144), (69), (54), (31), (13), (7), (5), (4), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling References for anti-GRB2 antibody (ABIN184670) Images
Blocking Peptide Blocking peptide for this product available: ABIN369579
Specificity This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Immunogen Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling References for anti-GRB2 antibody (ABIN184670) Images back to top
Antigen
Alternative Name GRB2 (GRB2 Antibody Abstract)
Background Synonyms: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, abundant SRC homology
Gene ID 2885, 14784, 81504
NCBI Accession NP_987102, NP_002077
Research Area Cancer
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling References for anti-GRB2 antibody (ABIN184670) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30 kDa band seen in Jurkat and NIH3T3 lysates. Recommended for use at 1-3 µg/mL.
IHC: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration: 0.1-0.3 µg/mL. Also islets of Langerhans in paraffin embedded Human Pancreas is specifically stained (please contact technical support for details).
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details References for anti-GRB2 antibody (ABIN184670) Images back to top
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-GRB2 antibody (ABIN184670)

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling Images back to top
Background publications

Lowenstein, Daly, Batzer, Li, Margolis, Lammers, Ullrich, Skolnik, Bar-Sagi, Schlessinger: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling References for anti-GRB2 antibody (ABIN184670) back to top
Supplier Images
Western Blotting (WB) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN184670) ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-Growth Factor Receptor-Bound Protein 2 (GRB2) (C-Term) antibody (ABIN184670) ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen ...