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anti-Rat (Rattus) GRB2 antibody for Immunocytochemistry

Recommended GRB2 Antibody (supplied by: Log in to see )

Antigen
Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
  • AA408164
  • ASH
  • Ash
  • EGFRBP-GRB2
  • GRB2
  • grb2
  • Grb3-3
  • MST084
  • MSTP084
  • NCKAP2
  • wu:fb18e12
  • wu:fb34e06
  • zgc:73077
Epitope
C-Term
36
18
15
7
6
6
6
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
46
46
10
8
4
3
2
2
2
2
1
1
Host
Rabbit
102
29
23
2
Clonality
Monoclonal
Conjugate
This GRB2 antibody is un-conjugated
5
4
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
134
68
54
30
9
8
5
3
3
2
1
Supplier
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Available images

Catalog No. ABIN189380
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN267213 ICC IF IHC (p) IHC Mouse IgG1 kappa Log in to see 2GB04
0.0009962841 ABIN2986379 ICC IF WB Rabbit IgG Log in to see Polyclonal

Similar anti-GRB2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 17 Antibodies 18 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 15 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies 3 Antibodies
Western Blotting (WB) 44 Antibodies 44 Antibodies

General

Antigen Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies
Epitope C-Term
(36), (18), (15), (7), (6), (6), (6), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(153), (46), (46), (10), (8), (4), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(102), (29), (23), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This GRB2 antibody is un-conjugated
(5), (4), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(134), (68), (54), (30), (9), (8), (5), (3), (3), (2), (1)
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Product Details anti-GRB2 Antibody

Target Details GRB2 Application Details Handling Images
Specificity This antibody can also detect the splice isoform GRB3-3 of GRB2.
Purification Protein A purified
Immunogen A synthetic peptide corresponding to residues in the C-terminus of human GRB2 was used as an immunogen.
Clone Y237
Isotype IgG

Target Details GRB2

Product Details anti-GRB2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name GRB2 / ASH (GRB2 Antibody Abstract)
Background The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that containsone src homology (SH2) domain and two SH3 domains (1). GRB2 associates withTyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays acrucial role in two separate insulin pathways leading from the insulin receptor to the Rasprotein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptorprotein, recruits Sos for Ras activation and subsequent signal transmission (3). Alternate Names: anti-Adapter protein GRB2 antibody, anti-ASH antibody, anti-Ash protein antibody,anti-EGFRBP GRB2 antibody, anti-Epidermal growth factor receptor binding proteinantibody, anti-Epidermal growth factor receptor binding protein GRB2 antibody,anti-GRB 2 antibody, anti-GRB2 adapter protein antibody, anti-Grb3 3 antibody,anti-Growth Factor Receptor Bound Protein 2 antibody, anti-Growth factor receptorbound protein 3 antibody, anti-HT027 antibody, anti-MST084 antibody, anti-MSTP084antibody, anti-Protein ASH antibody, anti-SEM5 antibody, anti-SH2/SH3 adapter GRB2antibody.
Gene Symbol: GRB2
Gene ID 2885, 14784, 81504
Pathways RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000
Protocol Protocol specific for GRB2 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN189380) Western blot analysis on 293 cell lysate using anti-GRB2 (C-term) RabMAb (catalog #11...
Immunohistochemistry (IHC) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN189380) Immunohistochemical staining of paraffin-embedded human ovarian carcinoma using anti-...
Immunofluorescence (IF) image for anti-GRB2 antibody (Growth Factor Receptor-Bound Protein 2) (C-Term) (ABIN189380) Immunofluorescent staining of HeLa cells using anti-GRB2 (C-term) RabMAb (catalog #11...