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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|BioImaging (BI)||2 Antibodies||2 Antibodies|
|ELISA||14 Antibodies||16 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||4 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies||15 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies||9 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies||3 Antibodies|
|Western Blotting (WB)||40 Antibodies||41 Antibodies|
|Antigen||Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GRB2 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-GRB2 AntibodyTarget Details GRB2 Application Details Handling Images
|Specificity||This antibody can also detect the splice isoform GRB3-3 of GRB2.|
|Purification||Protein A purified|
|Immunogen||A synthetic peptide corresponding to residues in the C-terminus of human GRB2 was used as an immunogen.|
Target Details GRB2Product Details anti-GRB2 Antibody Application Details Handling Images back to top
|Alternative Name||GRB2 / ASH (GRB2 Antibody Abstract)|
The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that containsone src homology (SH2) domain and two SH3 domains (1). GRB2 associates withTyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays acrucial role in two separate insulin pathways leading from the insulin receptor to the Rasprotein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptorprotein, recruits Sos for Ras activation and subsequent signal transmission (3). Alternate Names: anti-Adapter protein GRB2 antibody, anti-ASH antibody, anti-Ash protein antibody,anti-EGFRBP GRB2 antibody, anti-Epidermal growth factor receptor binding proteinantibody, anti-Epidermal growth factor receptor binding protein GRB2 antibody,anti-GRB 2 antibody, anti-GRB2 adapter protein antibody, anti-Grb3 3 antibody,anti-Growth Factor Receptor Bound Protein 2 antibody, anti-Growth factor receptorbound protein 3 antibody, anti-HT027 antibody, anti-MST084 antibody, anti-MSTP084antibody, anti-Protein ASH antibody, anti-SEM5 antibody, anti-SH2/SH3 adapter GRB2antibody.
Gene Symbol: GRB2
|Gene ID||2885, 14784, 81504|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
|Application Notes||Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-100, Immunohistochemistry 1:100-250, Immunohistochemistry-Paraffin 1:100-250, Immunoprecipitation 1:50, Western Blot 1:1000-10000|
Protocol specific for GRB2 Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top