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anti-Dog (Canine) HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 1240-1260
117
84
66
55
54
53
49
45
44
35
33
29
22
21
21
21
21
20
20
20
20
20
20
19
19
19
16
16
14
14
13
13
12
12
12
11
9
9
8
8
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine)
1348
426
260
23
13
4
3
3
3
3
2
2
1
1
1
Host
Mouse
991
503
29
17
10
5
1
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
54
45
41
18
18
10
10
10
9
9
8
8
8
8
8
7
7
7
7
7
7
7
7
3
2
2
2
Application
Western Blotting (WB)
678
599
337
323
300
295
214
125
107
90
87
21
17
14
6
6
5
5
5
3
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN126791
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN126788 WB Mouse IgG1 AA 860-880 Log in to see 24B5
2.9590793 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5
2.9590793 ABIN126787 WB Mouse IgG1 pThr686 Log in to see 7F8
2.9590793 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
2.9590793 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal
2.9590793 ABIN2957599 IHC WB Rabbit Log in to see Polyclonal
1 ABIN492348 WB Biotin Mouse IgG1 AA 1240-1260 Log in to see 19D2
1 ABIN492345 IF WB Biotin Mouse IgG1 pTyr1112 Log in to see 19G5
1 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
1 ABIN2755561 IC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
1 ABIN3070843 ICC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
1 ABIN2718011 IHC (p) WB Mouse IgG2a Log in to see UMAB33
1 ABIN2718012 IHC (p) WB Mouse IgG2b Log in to see UMAB34
1 ABIN2718013 IHC (p) WB Mouse IgG2b Log in to see UMAB35
1 ABIN2718016 IHC (p) WB Mouse IgG2b Log in to see UMAB34
1 ABIN2718017 IHC (p) WB Mouse IgG2b Log in to see UMAB35
1 ABIN2718015 IHC (p) WB Mouse IgG2a Log in to see UMAB33
1 ABIN2717975 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-HER2 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 14 Antibodies
Western Blotting (WB) 655 Antibodies 19 Antibodies 193 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Neutralization (Neut) 1 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 126 Antibodies 24 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 211 Antibodies 53 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 333 Antibodies 92 Antibodies 7 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1240-1260
(117), (84), (66), (55), (54), (53), (49), (45), (44), (35), (33), (29), (22), (21), (21), (21), (21), (20), (20), (20), (20), (20), (20), (19), (19), (19), (16), (16), (14), (14), (13), (13), (12), (12), (12), (11), (9), (9), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine)
(1348), (426), (260), (23), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(991), (503), (29), (17), (10), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (54), (45), (41), (18), (18), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (3), (2), (2), (2)
Application Western Blotting (WB)
(678), (599), (337), (323), (300), (295), (214), (125), (107), (90), (87), (21), (17), (14), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity This antibody specifically recognizes ErbB2 at 185 kDa.
Characteristics Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Peptide conjugated to hemocyaninEpitope: aa 1240-1260
Clone 19D2
Isotype IgG1

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Immunoblotting: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from untreated SKOV-3Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer PBS, 0.09 % Sodium Azide/PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 1240-1260) (ABIN126791) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1240-1260) antibody