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|Application / Reactivity||Human||Mouse (Murine)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||8 Antibodies||2 Antibodies||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||14 Antibodies|
|Western Blotting (WB)||679 Antibodies||27 Antibodies||248 Antibodies|
|Radioimmunoassay (RIA)||2 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies||1 Antibodies||1 Antibodies|
|Neutralization (Neut)||1 Antibodies|
|Luminex Assay (LMNX)||21 Antibodies|
|Intracellular Flow Cytometry (ICFC)||3 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||137 Antibodies||55 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||265 Antibodies|
|Antigen||V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies|
|Epitope||AA 1240-1260 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This HER2 antibody is un-conjugated Alternatives|
Western Blotting (WB)
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Product Details anti-HER2 AntibodyTarget Details HER2 Application Details Handling Images
|Specificity||This antibody specifically recognizes ErbB2 at 185 kDa.|
|Characteristics||Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2|
|Purification||Subsequent Ultrafiltration and Size Exclusion Chromatography.|
|Components||incl. positive Control|
|Immunogen||Peptide conjugated to hemocyaninEpitope: aa 1240-1260|
Target Details HER2Product Details anti-HER2 Antibody Application Details Handling Images back to top
|Alternative Name||CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)|
|Background||ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development|
Application DetailsProduct Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Immunoblotting: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control Provided. Cell lysate from untreated SKOV-3Description: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||PBS, 0.09 % Sodium Azide/PEG and Sucrose.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top