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anti-Dog (Canine) HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
pTyr1112
120
83
64
54
54
53
50
44
37
36
32
29
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
1322
417
260
23
13
4
3
3
3
3
2
2
1
1
1
Host
Mouse
969
495
25
18
5
5
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
52
45
41
17
17
10
10
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
680
574
331
323
301
281
235
174
104
90
86
21
17
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN126789
$ 533.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN126788 WB Mouse IgG1 AA 860-880 Log in to see 24B5
0.13341247 ABIN126791 WB Mouse IgG1 AA 1240-1260 Log in to see 19D2
0.13341247 ABIN126787 WB Mouse IgG1 pThr686 Log in to see 7F8
0.13341247 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN492345 IF WB Biotin Mouse IgG1 pTyr1112 Log in to see 19G5
0.0011728023 ABIN492348 WB Biotin Mouse IgG1 AA 1240-1260 Log in to see 19D2
0.0011728023 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal
0.0011728023 ABIN2755561 IC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
0.0011728023 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN2957599 IHC WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN3070843 ICC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal
0.0011728023 ABIN2718011 IHC (p) WB Mouse IgG2a Log in to see UMAB33
0.0011728023 ABIN2718013 IHC (p) WB Mouse IgG2b Log in to see UMAB35
0.0011728023 ABIN2718012 IHC (p) WB Mouse IgG2b Log in to see UMAB34
0.0011728023 ABIN2718016 IHC (p) WB Mouse IgG2b Log in to see UMAB34
0.0011728023 ABIN2718015 IHC (p) WB Mouse IgG2a Log in to see UMAB33
0.0011728023 ABIN2718017 IHC (p) WB Mouse IgG2b Log in to see UMAB35
0.0011728023 ABIN2717975 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

Similar anti-HER2 Antibodies

Application / Reactivity Human Dog (Canine) Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 660 Antibodies 19 Antibodies 197 Antibodies 166 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 176 Antibodies 47 Antibodies 26 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 235 Antibodies 47 Antibodies 55 Antibodies 7 Antibodies
Immunohistochemistry (IHC) 329 Antibodies 93 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pTyr1112
(120), (83), (64), (54), (54), (53), (50), (44), (37), (36), (32), (29), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(1322), (417), (260), (23), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(969), (495), (25), (18), (5), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (52), (45), (41), (17), (17), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2)
Application Immunofluorescence (IF), Western Blotting (WB)
(680), (574), (331), (323), (301), (281), (235), (174), (104), (90), (86), (21), (17), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity This antibody specifically recognizes erbB2 phosphorylated at Tyrosine 1112 at 185 kDa.
Characteristics Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
Purification Subsequent Ultrafiltration and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Synthetic phosphopetide conjugated to KLH. Epitope: phospho-Tyr1112LQRpYSED
Clone 19G5
Isotype IgG1

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. Dysregulation of erbB2 and/or activation of downstream signaling pathways has been implicated in many human cancers. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248.Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of Thr 686 and Ser 1113 interferes with erbB2 endocytosis and degradation.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended buffer: Casein/Tween 20 based blocking and blot incubation buffer. Use cell lysate from EGF-treated SKOV-3 cells as positive control (please see Protocolsbelow for details). Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol POSITIVE CONTROL CELL LYSATE: SKOV-3 EGF treatedFormat: Lyophilized cell lysate from SKOV-3 cells. Serum starved cells were treated for 15min with EGF. Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Applications: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as immunoprecipitation. Storage: In aliquots at -20°C. Avoid repeated freezing and thawing.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS containing 0.09 % Sodium Azide/PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (pTyr1112) (ABIN126789) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1112) antibody