anti-Human HER2 antibody for Enzyme Immunoassay

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
Tyr1248
101
95
72
69
62
55
55
48
46
35
26
22
22
21
20
20
20
20
20
20
20
20
20
19
19
19
18
17
15
14
13
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1373
494
325
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
1005
512
30
16
12
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
54
46
41
19
19
12
12
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
715
602
318
314
309
309
267
132
112
102
89
21
18
18
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN359842
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN126790 EIA IF WB Mouse IgG1 pSer1113 Log in to see 9-00E-010
1 ABIN5552237 EIA FACS IHC (p) WB Rabbit Ig Fraction AA 30-59, N-Term Log in to see Polyclonal
1 ABIN5554778 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal
1 ABIN782315 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-001
1 ABIN492347 EIA IF WB Biotin Mouse IgG1 pSer1113 Log in to see 9-00E-010
1 ABIN5552238 EIA IHC (p) Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN1106312 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 6C2 2
1 ABIN265463 EIA WB Rabbit Log in to see Polyclonal
1 ABIN265464 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN265465 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal
1 ABIN115506 EIA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1106311 EIA IHC (p) WB Mouse Log in to see 6C2B12 2
1 ABIN121528 EIA IHC (fro) WB Rabbit pThr686 Log in to see Polyclonal
1 ABIN5541742 EIA IHC (p) Goat Internal Region Log in to see Polyclonal
1 ABIN5541743 EIA IHC (p) Goat AA 182-194, Internal Region Log in to see Polyclonal
1 ABIN641228 CA DB EIA IA ISt ELISA WB Rabbit IgG pTyr877 Log in to see Polyclonal
1 ABIN2717975 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope Tyr1248
(101), (95), (72), (69), (62), (55), (55), (48), (46), (35), (26), (22), (22), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (18), (17), (15), (14), (13), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1373), (494), (325), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(1005), (512), (30), (16), (12), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (54), (46), (41), (19), (19), (12), (12), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(715), (602), (318), (314), (309), (309), (267), (132), (112), (102), (89), (21), (18), (18), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN359842) Images
Specificity This antibody reacts to ERBB2.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y1248 of human ERBB2.
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN359842) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 9606
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling ProductDetails: References for anti-HER2 antibody (ABIN359842) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details ProductDetails: References for anti-HER2 antibody (ABIN359842) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-HER2 antibody (ABIN359842)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling ProductDetails: References for anti-HER2 antibody (ABIN359842) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (Tyr1248) antibody (ABIN359842) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Pho...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (Tyr1248) antibody (ABIN359842) Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody. 293 cel...