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anti-Human HER2 antibody for Enzyme Immunoassay

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
Tyr1248
116
82
64
54
54
51
51
44
35
32
32
29
21
20
20
20
20
19
19
19
19
19
19
18
18
18
15
14
14
14
13
13
13
12
10
10
9
8
8
8
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1145
407
234
24
12
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
936
351
19
18
5
5
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
67
62
53
45
45
41
9
9
8
8
8
8
8
8
8
2
2
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
623
514
323
319
215
182
133
112
90
45
21
16
12
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN359842
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008913908 ABIN359840 EIA FACS IHC (p) WB Rabbit Ig AA 30-59, N-Term Log in to see Polyclonal 4
0.0008913908 ABIN359843 EIA IHC (p) Rabbit Ig Log in to see Polyclonal 4
0.0008913908 ABIN499802 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal 5
0.0008913908 ABIN265464 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008913908 ABIN265465 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008913908 ABIN126790 EIA IF WB Mouse IgG1 pSer1113 Log in to see 9-00E-010
0.0008913908 ABIN492347 EIA IF WB Biotin Mouse IgG1 pSer1113 Log in to see 9-00E-010
0.0008913908 ABIN782315 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-001
0.0008913908 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal
0.0008913908 ABIN1106312 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 6C2 2
0.0008913908 ABIN265463 EIA WB Rabbit Log in to see Polyclonal
0.0008913908 ABIN115506 EIA WB Rabbit N-Term Log in to see Polyclonal
0.0008913908 ABIN1106311 EIA IHC (p) WB Mouse Log in to see 6C2B12 2
0.0008913908 ABIN121528 EIA IHC (fro) WB Rabbit pThr686 Log in to see Polyclonal
0.0008913908 ABIN641228 CA DB EIA IA ISt ELISA WB Rabbit IgG pTyr877 Log in to see Polyclonal
0.0008913908 ABIN2717975 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-HER2 antibody for Enzyme Immunoassay

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 379 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 133 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 215 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 90 Antibodies
Immunohistochemistry (IHC) 317 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 182 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope Tyr1248
(116), (82), (64), (54), (54), (51), (51), (44), (35), (32), (32), (29), (21), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (15), (14), (14), (14), (13), (13), (13), (12), (10), (10), (9), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1145), (407), (234), (24), (12), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(936), (351), (19), (18), (5), (5)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(67), (62), (53), (45), (45), (41), (9), (9), (8), (8), (8), (8), (8), (8), (8), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(623), (514), (323), (319), (215), (182), (133), (112), (90), (45), (21), (16), (12), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359842) Images
Specificity This antibody reacts to ERBB2.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y1248 of human ERBB2.
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN359842) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 9606
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN359842) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN359842) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-HER2 Antibody (ABIN359842)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359842) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (Tyr1248) (ABIN359842) Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Pho...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (Tyr1248) (ABIN359842) Western blot analysis of ERBB2(arrow) using rabbit polyclonal ERBB2 Antibody. 293 cel...