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anti-Human HER2 antibody for Flow Cytometry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 30-59, N-Term
117
84
66
55
54
53
49
45
44
35
33
28
22
21
21
21
21
20
20
20
20
20
20
19
19
19
16
16
14
14
13
13
12
12
11
11
9
9
8
8
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1348
427
260
24
13
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
990
504
29
17
10
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
54
45
41
18
18
10
10
10
9
9
8
8
8
8
8
7
7
7
7
7
7
7
7
3
2
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
678
599
337
323
300
294
213
125
107
90
87
21
16
14
6
6
5
5
5
3
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN359840
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.747074 ABIN391948 FACS IF IHC (p) WB Rabbit Ig Log in to see Polyclonal 4
13.747074 ABIN969111 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2 2
13.747074 ABIN4896631 FACS Allophycocyanin Mouse IgG2b AA 23-652 Log in to see 191924 1
13.747074 ABIN4896633 FACS PE Mouse IgG2b AA 23-652 Log in to see 191924 3
13.747074 ABIN4899767 ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
13.747074 ABIN4899768 ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
13.747074 ABIN1106312 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 6C2 2
13.747074 ABIN2663396 FACS PE Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2658634 FACS ICFC APC Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2659248 FACS PE-Cy7 Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2657409 FACS IF Alexa Fluor 488 Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2660094 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2657764 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2661687 Func ICC FACS IF ICFC IP WB FITC Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN4896632 FACS Alexa Fluor 488 Mouse IgG2b AA 23-652 Log in to see 191924
13.747074 ABIN4896634 FACS Alexa Fluor 647 Mouse IgG2b AA 23-652 Log in to see 191924
13.747074 ABIN4896635 FACS Alexa Fluor 594 Mouse IgG2b AA 23-652 Log in to see 191924
13.747074 ABIN2664603 FACS Mouse IgG1 kappa Log in to see 24D2 6
13.747074 ABIN2167755 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2
13.747074 ABIN1995356 FACS APC Rabbit IgG AA 1-652 Log in to see 511

Top referenced anti-HER2 antibody for Flow Cytometry

Similar anti-HER2 Antibodies

Application / Reactivity Human
Cellular Assay (CA) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies
Dot Blot (DB) 192 Antibodies
ELISA 464 Antibodies
ELISA (Capture) 3 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 285 Antibodies
Functional Studies (Func) 5 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 87 Antibodies
Immunoelectron Microscopy (IEM) 6 Antibodies
Immunofluorescence (IF) 300 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 90 Antibodies
Immunohistochemistry (IHC) 333 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 5 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 107 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 30-59, N-Term
(117), (84), (66), (55), (54), (53), (49), (45), (44), (35), (33), (28), (22), (21), (21), (21), (21), (20), (20), (20), (20), (20), (20), (19), (19), (19), (16), (16), (14), (14), (13), (13), (12), (12), (11), (11), (9), (9), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1348), (427), (260), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(990), (504), (29), (17), (10), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (54), (45), (41), (18), (18), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (3), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(678), (599), (337), (323), (300), (294), (213), (125), (107), (90), (87), (21), (16), (14), (6), (6), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359840) Images
Specificity This antibody recognizes Human HER2/ErbB2. Other species not tested.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen KLH conjugated synthetic peptide between 30~59 amino acids from the N-terminal region of Human HER2/ErbB2 . Genename: ERBB2
Isotype Ig

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN359840) Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An Interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residuesSynonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064, 9606
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN359840) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN359840) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS, 0.09 % (W/V) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-HER2 Antibody (ABIN359840)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Ehsani, Low, Wallace, Wu: "Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization." in: Genomics, Vol. 15, Issue 2, pp. 426-9, 1993

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

Coussens, Yang-Feng, Liao, Chen, Gray, McGrath, Seeburg, Libermann, Schlessinger, Francke: "Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene." in: Science (New York, N.Y.), Vol. 230, Issue 4730, pp. 1132-9, 1985

Semba, Kamata, Toyoshima, Yamamoto: "A v-erbB-related protooncogene, c-erbB-2, is distinct from the c-erbB-1/epidermal growth factor-receptor gene and is amplified in a human salivary gland adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 19, pp. 6497-501, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN359840) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) Flow Cytometric analysis of MCF-7 cells using HER2/ErbB2 Antibody (N-term) Cat.-No AP...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) (LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the prima...
Western Blotting (WB) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (AA 30-59) (ABIN359840) (LEFT)Western blot analysis of anti-ErbB2 Pab in SKBR-3 cell lysate. ErbB2 (arrow) wa...