anti-Human HER2 antibody for Flow Cytometry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 23-652
102
94
71
69
60
55
54
48
45
35
26
22
22
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
15
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1368
491
320
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Mouse
1002
510
32
16
12
5
1
Clonality
Monoclonal
Conjugate
This HER2 antibody is conjugated to PE
76
66
59
54
46
41
19
19
12
12
11
10
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Flow Cytometry (FACS)
712
603
315
313
309
304
269
133
112
102
89
21
19
18
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4896633
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.827366 ABIN4896631 FACS Allophycocyanin Mouse IgG2b AA 23-652 Log in to see 191924 1
13.827366 ABIN4899767 CyTOF ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
13.827366 ABIN4899768 CyTOF ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
13.827366 ABIN391948 FACS IF IHC (p) WB Rabbit Ig Log in to see Polyclonal 4
13.827366 ABIN969111 FACS IHC ELISA WB Mouse IgG1 AA 750-987 Log in to see 6C2 2
13.827366 ABIN4896635 FACS Alexa Fluor 594 Mouse IgG2b AA 23-652 Log in to see 191924
13.827366 ABIN4896632 FACS Alexa Fluor 488 Mouse IgG2b AA 23-652 Log in to see 191924
13.827366 ABIN4896634 FACS Alexa Fluor 647 Mouse IgG2b AA 23-652 Log in to see 191924
13.827366 ABIN1995356 FACS APC Rabbit IgG AA 1-652, Extracellular Domain Log in to see 511
13.827366 ABIN1995358 FACS IF IF/ICC PE Rabbit IgG AA 1-652, Extracellular Domain Log in to see 511
13.827366 ABIN1106312 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 6C2 2
13.827366 ABIN1995348 FACS PE Mouse IgG1 AA 1-652 Log in to see 9G1D4B10
13.827366 ABIN1995346 FACS FITC Mouse IgG1 AA 1-652 Log in to see 9G1D4B10
13.827366 ABIN1995350 FACS IF IF/ICC FITC Mouse IgG1 AA 1-652 Log in to see 07
13.827366 ABIN4309238 FACS Mouse IgG1 kappa AA 23-652, C-Term Log in to see 3C2-4
13.827366 ABIN4309126 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4F10
13.827366 ABIN2660094 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see 24D2 6
13.827366 ABIN2661687 Func ICC FACS IF ICFC IP WB FITC Mouse IgG1 kappa Log in to see 24D2 6
13.827366 ABIN2658634 FACS ICFC APC Mouse IgG1 kappa Log in to see 24D2 6
13.827366 ABIN2659248 FACS PE-Cy7 Mouse IgG1 kappa Log in to see 24D2 6

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 23-652
(102), (94), (71), (69), (60), (55), (54), (48), (45), (35), (26), (22), (22), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (15), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1368), (491), (320), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(1002), (510), (32), (16), (12), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is conjugated to PE
(76), (66), (59), (54), (46), (41), (19), (19), (12), (12), (11), (10), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Flow Cytometry (FACS)
(712), (603), (315), (313), (309), (304), (269), (133), (112), (102), (89), (21), (19), (18), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4896633) Images
Specificity Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
Purification Protein A or G purified from hybridoma culture supernatant
Immunogen Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2, Thr23-Thr652, Accession # P04626
Clone 191924
Isotype IgG2b

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN4896633) Images back to top
Antigen
Alternative Name ErbB2/Her2 (ERBB2 Antibody Abstract)
Background Alternative Names: CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGL, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN4896633) Images back to top
Application Notes Flow Cytometry 10 uL/10^6 cells
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN4896633) Images back to top
Format Liquid
Buffer Supplied in a saline solution containing BSA and Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Protect from light. Do not freeze. 12 months from date of receipt,2°C to 8°C as supplied.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN4896633)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Sie, den Dunnen, Lourens, Meeuwsen-de Boer, Scherpen, Zomerman, Kampen, Hoving, de Bont: "Growth-factor-driven rescue to receptor tyrosine kinase (RTK) inhibitors through Akt and Erk phosphorylation in pediatric low grade astrocytoma and ependymoma." in: PLoS ONE, Vol. 10, Issue 3, pp. e0122555, 2015 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Nakanishi, Chumsri, Khakpour, Brodie, Leyland-Jones, Hamburger, Ross, Burger: "Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling." in: British journal of cancer, Vol. 102, Issue 5, pp. 815-26, 2010 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Dai, Tiwari, Wu, Su, Wang, Liu, Ashby, Huang, Robey, Liang, Chen, Shi, Ambudkar, Chen, Fu: "Lapatinib (Tykerb, GW572016) reverses multidrug resistance in cancer cells by inhibiting the activity of ATP-binding cassette subfamily B member 1 and G member 2." in: Cancer research, Vol. 68, Issue 19, pp. 7905-14, 2008 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4896633) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (PE) (ABIN4896633) Detection of ErbB2/Her2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breas...