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anti-Human HER2 antibody for Immunofluorescence

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • c-erbB2
  • c-neu
  • CD340
  • Erbb-2
  • HER-2
  • HER-2/neu
  • HER2
  • mKIAA3023
  • MLN 19
  • NEU
  • Neu
  • NGL
  • TKR1
Epitope
pThr686
117
83
64
54
53
51
50
44
37
35
32
29
20
20
20
20
20
19
19
19
19
19
19
18
18
18
16
14
14
14
13
13
12
12
10
9
9
8
8
8
8
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1314
414
259
24
13
4
3
3
3
3
2
2
1
1
1
Host
Rabbit
959
497
25
18
5
5
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
74
65
60
52
45
41
17
17
10
10
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
2
2
2
Application
Immunofluorescence (IF), ELISA
677
576
331
323
301
282
231
173
103
90
86
21
17
6
6
5
5
5
3
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN1531590
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29013318 ABIN1534423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.29013318 ABIN1532607 IF ELISA Rabbit IgG Log in to see Polyclonal 3
0.29013318 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.29013318 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
0.29013318 ABIN1881312 DB IF WB Rabbit pTyr1005 Log in to see Polyclonal 3
0.29013318 ABIN1881313 DB IF WB Rabbit pTyr1196 Log in to see Polyclonal 3
0.29013318 ABIN2455003 IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 1D9
0.29013318 ABIN2455009 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 1C5
0.29013318 ABIN2455013 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 12G4
0.29013318 ABIN2455012 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 9G2
0.29013318 ABIN2454964 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 4F10
0.29013318 ABIN2455010 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 11A7
0.29013318 ABIN2455011 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 3F11
0.29013318 ABIN782315 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-001
0.29013318 ABIN1532624 IF ELISA Rabbit IgG Log in to see Polyclonal
0.29013318 ABIN1539721 DB IF Rabbit Ig pThr1172 Log in to see Polyclonal
0.29013318 ABIN1870165 IF WB Rabbit IgG pTyr1221, pTyr1222 Log in to see Polyclonal
0.29013318 ABIN1870167 IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal
0.29013318 ABIN1539720 DB IF Rabbit Ig pThr1166 Log in to see Polyclonal
0.29013318 ABIN1539785 DB IF WB Rabbit Ig pTyr1127 Log in to see Polyclonal

Top referenced anti-HER2 antibody for Immunofluorescence

Similar anti-HER2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 654 Antibodies 194 Antibodies 165 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 175 Antibodies 46 Antibodies 25 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 229 Antibodies 46 Antibodies 54 Antibodies
Immunohistochemistry (IHC) 328 Antibodies 93 Antibodies 89 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 103 Antibodies 3 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pThr686
(117), (83), (64), (54), (53), (51), (50), (44), (37), (35), (32), (29), (20), (20), (20), (20), (20), (19), (19), (19), (19), (19), (19), (18), (18), (18), (16), (14), (14), (14), (13), (13), (12), (12), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1314), (414), (259), (24), (13), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(959), (497), (25), (18), (5), (5)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(74), (65), (60), (52), (45), (41), (17), (17), (10), (10), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2)
Application Immunofluorescence (IF), ELISA
(677), (576), (331), (323), (301), (282), (231), (173), (103), (90), (86), (21), (17), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN1531590) Images
Specificity HER2 (Phospho-Thr686) Antibody detects endogenous levels of HER2 only when phosphorylated at Thr686.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Thr686.
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN1531590) Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background Molecular weight of target protein: 137 kDa
Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein- tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kinase ErbB2, p185erbB2
Molecular Weight 137 kDa
Gene ID 2064
OMIM 164870
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN1531590) Images back to top
Application Notes IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.446352 (NCBI Gene Symbol: ERBB2)

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN1531590) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-HER2 Antibody (ABIN1531590)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

King, Kraus, Aaronson: "Amplification of a novel v-erbB-related gene in a human mammary carcinoma." in: Science (New York, N.Y.), Vol. 229, Issue 4717, pp. 974-6, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN1531590) back to top
Supplier Images
Immunofluorescence (IF) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (pThr686) (ABIN1531590) Immunofluorescence analysis of HuvEc cells, using HER2 (Phospho-Thr686) Antibody. The...