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anti-Human HER2 antibody for Immunofluorescence

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
pThr686
100
82
70
63
56
53
52
46
44
35
27
22
22
21
21
21
21
20
20
20
20
20
20
19
19
19
18
16
15
14
13
13
12
12
10
8
8
8
8
8
7
7
7
6
6
6
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1354
498
332
35
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Rabbit
995
503
29
17
10
5
1
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
73
64
60
54
45
41
18
18
10
10
10
9
9
8
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunofluorescence (IF), ELISA
706
608
327
322
307
298
240
128
111
102
87
21
17
14
6
5
5
5
5
3
3
3
3
2
2
1
1
1
1
Options
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Catalog No. ABIN1531590
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.237935 ABIN1534423 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
12.237935 ABIN1532607 IF ELISA Rabbit IgG Log in to see Polyclonal 3
12.237935 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
12.237935 ABIN967787 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
12.237935 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 1
12.237935 ABIN1881312 DB IF WB Rabbit pTyr1005 Log in to see Polyclonal 3
12.237935 ABIN1881313 DB IF WB Rabbit pTyr1196 Log in to see Polyclonal 3
12.237935 ABIN2455009 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 1C5
12.237935 ABIN2455003 IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 1D9
12.237935 ABIN2455010 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 11A7
12.237935 ABIN2455011 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 3F11
12.237935 ABIN2455013 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 12G4
12.237935 ABIN2455012 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 9G2
12.237935 ABIN2454964 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 4F10
12.237935 ABIN782315 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-001
12.237935 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
12.237935 ABIN1539721 DB IF Rabbit Ig pThr1172 Log in to see Polyclonal
12.237935 ABIN1532624 IF ELISA Rabbit IgG Log in to see Polyclonal
12.237935 ABIN1870165 IF WB Rabbit IgG pTyr1221, pTyr1222 Log in to see Polyclonal
12.237935 ABIN1870167 IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal

Top referenced anti-HER2 antibody for Immunofluorescence

Similar anti-HER2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies 2 Antibodies 2 Antibodies
Cytometry by Time of Flight (CyTOF) 14 Antibodies 2 Antibodies
Western Blotting (WB) 678 Antibodies 249 Antibodies 225 Antibodies
Radioimmunoassay (RIA) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies 1 Antibodies 1 Antibodies
Neutralization (Neut) 1 Antibodies
Luminex Assay (LMNX) 21 Antibodies
Intracellular Flow Cytometry (ICFC) 3 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 129 Antibodies 55 Antibodies 47 Antibodies

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pThr686
(100), (82), (70), (63), (56), (53), (52), (46), (44), (35), (27), (22), (22), (21), (21), (21), (21), (20), (20), (20), (20), (20), (20), (19), (19), (19), (18), (16), (15), (14), (13), (13), (12), (12), (10), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1354), (498), (332), (35), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(995), (503), (29), (17), (10), (5), (1)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(73), (64), (60), (54), (45), (41), (18), (18), (10), (10), (10), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunofluorescence (IF), ELISA
(706), (608), (327), (322), (307), (298), (240), (128), (111), (102), (87), (21), (17), (14), (6), (5), (5), (5), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN1531590) Images
Specificity HER2 (Phospho-Thr686) Antibody detects endogenous levels of HER2 only when phosphorylated at Thr686.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Thr686.
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 Antibody (ABIN1531590) Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background Molecular weight of target protein: 137 kDa
Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein- tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kinase ErbB2, p185erbB2
Molecular Weight 137 kDa
Gene ID 2064
OMIM 164870
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 Antibody (ABIN1531590) Images back to top
Application Notes IF: 1:100-1:500
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.446352 (NCBI Gene Symbol: ERBB2)

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 Antibody (ABIN1531590) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-HER2 Antibody (ABIN1531590)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

King, Kraus, Aaronson: "Amplification of a novel v-erbB-related gene in a human mammary carcinoma." in: Science (New York, N.Y.), Vol. 229, Issue 4717, pp. 974-6, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 Antibody (ABIN1531590) back to top
Supplier Images
Immunofluorescence (IF) image for anti-HER2 antibody (V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian)) (pThr686) (ABIN1531590) Immunofluorescence analysis of HuvEc cells, using HER2 (Phospho-Thr686) Antibody. The...